EMAGE mouse gene expression spatial database

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EMAGE:19514

Csrnp2 cysteine-serine-rich nuclear protein 2 ( MGI:2386852)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004949. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004949_01	euxassay_004949_02	euxassay_004949_03	euxassay_004949_04

euxassay_004949_05	euxassay_004949_06	euxassay_004949_07	euxassay_004949_08	euxassay_004949_09

euxassay_004949_10	euxassay_004949_11	euxassay_004949_12	euxassay_004949_13	euxassay_004949_14

euxassay_004949_15	euxassay_004949_16	euxassay_004949_17	euxassay_004949_18	euxassay_004949_19

euxassay_004949_20	euxassay_004949_21	euxassay_004949_22	euxassay_004949_23	euxassay_004949_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19514_wholemount_strong.wlz

19514_wholemount_moderate.wlz

19514_wholemount_weak.wlz

19514_wholemount_possible.wlz

19514_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19514_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6949
Entity Detected:	Csrnp2, cysteine-serine-rich nuclear protein 2 ( MGI:2386852)
Sequence:	sense strand is shown >T6949 GTTCTGGTCTTCGCTGCCCGGGGAGCCAGAGTGCAGAGCCGCCCGACCCCCGCCGCCTGCCGGCCGGCCC ACACACCCTGCAGCCCCGAGAGCCACGCGACGGGCCTGGGAGCCGGCACGCCTCGGTCTCCCCTCCTTGC ACACGGGCTGAGTAGGCAGCGGGGGCGGCGGAGACCGTGACGGGAGAAGCGGCTGCGGCGGGGCCGAGTC CTCATGTTCCTGGCTAGCTGGTGGTGAGCAGCTTGGGGCCATCGTGCTTGGCTCCCCGCGGACAACTTTG GAAGCCAATGGATGCGTTCTCCGGCTCAGGCCTCAAGAGGAAATTCGATGATGTGGACGTGGGCTCGTCA GTCTCCAACTCAGATGATGAGATGTCCAGCAGTGATAGTGCGGACAGCTGCGACAGCCTTAACCCTCCTA CGACTGCCAGCTTCACACCAACATCCATCCTGAAGAGGCAGAAGCAGCTACGGAGGAAGAATGTCCGCTT TGACCAGGTGACTGTGTACTACTTCGCCCGGCGACAGGGCTTCACCAGCGTGCCCAGCCAGGGTGGGAGC TCCTTGGGAATGGCCCAACGCCATAACTCTGTACGCAGCTACACCCTCTGTGAGTTTGCGCAAGAGCAGG AGGTGAACCATAGAGAGATTCTCCGTGAGCACCTGAAGGAGGAGAAACTTCACGCCAAGAAAATGAAGCT GACGAAGAACGGGACCGTGGAGTCAGTGGAGGCCGACGGCTTGACACTCGATGATGTCTCGGACGAGGAC ATCGACGTGGAGAATGTGGAGGTGGACGACTACTTCTTCCTTCAGCCCCTGCCCACCAAACGGCGGCGGG CCCTGCTCAGGGCTTCCGGGGTCCACCGGATTGACGCCGAAGAGAAGCAAGAACTCAGAGCCATCCGCCT GTCTCGGGAAGAGTGTGGTTGTGACTGTCGGCTGTACTGTGACCCAGAGGCATGCGCCTGTAGCCAGGCT GGGATTAAATGCCAGGTGGATCGAATGTCTTTTCCGTGTGGCTGCTCCAGGGATGGCTGTGGAAACATGG CTGGGCGAATTGAGTTTAACCCGATCCGAGTGCGGACTCACTACCTCCACACCATCATGAAGCTGGAGCT GGAGAGCAAGCGGCAGGTGAGTCGCCCAGCAGCAGAGGAGGAGCCCCTGCCGGGAGCGCAGAGCTCGCAG ACACAGGACTTCCAGGAGTTCATCGCCGAGAACGAGACTGCAGTGATGCATCTGCAGAGTGCAGAGGAGC TGGAGCGGCTCAAGGCCGAGGAGGACTCGAGTGGCTCTAGCGCTAGCCTGGACTCCAGCATGGAGAGCCT GGGCGTGTGCATCCTGGAGGAACCCCTGGCTGTTCCCCAAGAGCTGTGCCCAGGCCTCGCGGCCCCCATT CTCATCCAGGCTCAGCTGCCCCCAGGTTCCTCCGTCCTGTGTTTTACTGAGAACTCAGAACACCCAGCTG CCTCCCCGATGAGCAGCCCGTCTTACTTGAACAGTGGACCTCTGGTGTACTACCAGGTAGAGCAGAGGCC AGTGGTGGGAGTGAAAGCAGAGTCCGGGTCAGAGGAAGGCCCAGCCTCCTTCCCCAAAGAGAAAGATCTG AGTGTTTTCTCGCTCCCTGTTACCTCACTGGTGGCTTGCGGCCCTTCAGCCTCAGCTGCTCTCTGTAAGC CAGAGGTGGGGAAAACATCCAGCCTCAACAAACTCCTGCCCGAGGACTGCGGCCTTAAGGAGCCTGAGAG CGAGGACTTGCACCCCTCTTGGTCTCCCTCAAGCCTACCCTTCCGAACGGACAATGAAGAAGGCTGCGGA GTGCAGAACTCCCAGCAGAGTGAGGACCGGACCTCTGAAGACTCAGCCCTAGAACTCCCTCTGGCAGTGT GACCTGAGATACATGTCCATTCTCTATTTATTATCTAACACCATTTCAAAACAAGACTGCTGCTCCCAGG TTATTTTGTTGGAAGTCTTAGGAAAATGGGTAGGAAAGGATTGTTAGTCCATGTGTTTCTGAGAGAGCGT TCCCAGGAGACCAGCCCCAGCTGCAGTGTCCCGTACCGAGCTCTCTGGGCCGCCAGAACAAAGATGTCGA TCACAGAGTGAGCTGGGCAGCTCAAGCAGCTCTTTATGCCTAGTGCCAGAACCAAGGATGCGAGCAGCTC CCTGCACCACATGCTGGCTCCTCAAACACCCTGTCTTCTTGTAGCAGCCACTTTAGTGGAAAGGCCACTC AGCTGAGTCTAGAACGGAGAGGAGGCCCCAGAAGAGTGCTTCGATACAAAACTCCCAGGAGCTTTAAACG TTTATTCTGCCCCAACCCCTTGGTGTTTCCTGACCTGATCTGCAAACTGGGGATCCCTGGTCTGTACCTG AATTCTTTCTTTCATATGTGCTTCGGCACGAGAGTGAAACATCTGTCTTGGAAGGTGAAATGCAGGCTGG GTAGGGATGTGACTTGCACATGGGGCAGGGTCCAGTATGGAACAGTCTGGAAGATGGTTTTGTCAAGCTT GCTCTCTCACCTAGTGCAACACACACACACATCCACTCTCTCCTTTTGGTGGGGGGTCTTGGCTCTGAAT GGAATGTGCACTGCAGTACAGGTCATCGAGACATAGGACTCTTCCCTCTGTAACTGCCCTCACGGCCATT CTTGGTTGTGTGGGGTTAGAAAGTTCTTTGGAGTTTACAGTTCAATCCCAAGAGTGGCACGTGTGTTTTG GAGCCTTCCATCTTGGAGATTCATGAAAACTGGAAGTCAGACTGCTTCTTAGTTATGACATAGGAGTCCC ACATTCCAGAGGATTCCGGATCACATTATAATAATGACGTGGATGCCTGGGACTCAGACTCAACACTTAC CTCAGCAGAAGAGCTGCTCCTCTCGTGAGCTCGGGCAGTTCCGAGGTCCCCATTTAGCACGGGCTTCTTG ATTGGCAGTGCTACCTCTGCCTCCTGGTTAAATGGCGGCAGTAGGAGCAAAACGCTTTTCTCTTATCATT CTTTACCAAGCATCAAAACCACAAACTTGTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCT TTTCTTTTCTTTTTTCTTTTTTTTTTTTTAGGCAGTGTGTTGCTAAACAGCCCTGGCTGGCCTGGGACTT GCTATGTAGACCAGGCTGGCCTCCAACCTAGTCATGCATCCTGAGTGTTGTGACTGCAGGTGTACATCTC TTCACTTGGCTTTATCTATTTATTTATTTTTGGTGAGATAATAAGGCCCTGGTGTCATAAACAATCAATG TATGTGGCAGCAGAAACATCTGAAAAAATCCCTGAATCCTAAGCCCGGAGGACCTTGTATTTAACTGGAT GCTGGTGTTCTGCCCATTCTGCCACTGTCTCTTCTTGGGTTTCATTGGCTTTTGTTTTTGTTTTTGTTTT TTTGGCTGCACTTCTTAAGTGAGTGCATAGGCTCTCTCTCCAGTCTCACTAGAGTTATCTGAGAAGGTCA AGGTGTTCTCAACCCTGGCTAGACCTCTCAGACTCAAGCTTAGCTGAGATGATGTTGATGCAAAGAAGAA TCTTTTTGAAAGTTTGTTGGGACAGGAGGGGACTCTGCCCCCTACCTCCTTAAGACTCCAACTTTCTTAG CCCTTAGATTTGGATTTTCATTAAGAAACGTAAAGACTAGCCAACCCCAAGATATTTCTATATAACTAAT TTCTTATTCTAAATGCATTTCATTAATTCCATGTCGATTCAAACTGACCCCAACTTCTGAGTGCTAAACT TAGCGTTGACAATGTCAAGCAAAGAACCTCTTCTACAACTCAGTCCATCTCAGTTCTGGTGCTACCTGAG TCGGACACGATGTATGGTTCCTGGTTGCCGGGACGGCTGGGCCTGTGTCACAGATGCAGAGCCAGTCCCT CAGTCTAGGCCTAGAACTGTGGTGCACTGCTGCAGCTCTTGGACACCGTCTCCTCGGATTCTGCACACAG GTTGGACTCTCAATGTGAACCCAAAGCTTGTCTGGCTCTCTGAAAACAACTTTTGCTCTTTAGGTTCTTT ACATTGTAATAATGCTGTATTTAAACAGAATATTTAAATGTAATATTAAAGAAATATTGAAGAGAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3985077 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3985077 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19516 same embryo
	EMAGE:19512 same embryo
	EMAGE:19515 same embryo
	EMAGE:19513 same embryo
	EMAGE:19511 same embryo
	EurExpress:euxassay_004949 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source