EMAGE mouse gene expression spatial database

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EMAGE:19538

Hlcs holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) ( MGI:894646)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004961. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004961_01	euxassay_004961_02	euxassay_004961_03	euxassay_004961_04

euxassay_004961_05	euxassay_004961_06	euxassay_004961_07	euxassay_004961_08	euxassay_004961_09

euxassay_004961_10	euxassay_004961_11	euxassay_004961_12	euxassay_004961_13	euxassay_004961_14

euxassay_004961_15	euxassay_004961_16	euxassay_004961_17	euxassay_004961_18	euxassay_004961_19

euxassay_004961_20	euxassay_004961_21	euxassay_004961_22	euxassay_004961_23	euxassay_004961_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19538_wholemount_strong.wlz

19538_wholemount_moderate.wlz

19538_wholemount_weak.wlz

19538_wholemount_possible.wlz

19538_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19538_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7978
Entity Detected:	Hlcs, holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) ( MGI:894646)
Sequence:	sense strand is shown >T7978 ACATTTGTGACAGAGAGCATTTGGATACAAGGTACCAGTTTACAAGAGCCATCATCTTCAGAAACCGTTG TAAAATGGTCTGACTGCTGTCTGCCGTTAGCCTGCCGACCCGGGGACCCCTACCAGTTAATTGCTAAGGC AAGCGTGGACAACTTCAGCAAGCTTGGGGTGGCCTTCATGGAGGACAGGCTCCAGATGGACAACGGCCTG ATAGCCCAGAAGATCGTTTCAGTCCACTTAAAGGACCCTGCTCTGAAGGAACTCGGCAAAGCCTCCGACA AGCAAGTGCAGGGCCCGCCCCCGGGCCCAGAAGCTTCCCCCGAGGCCCAGCCTGCACAAGGTGTCATGGA GCATGCTGGCCAAGGTGATTGCAAGGCCGCCGGTGAAGGCCCTTCCCCACGGAGGAGGGGCTGTGCCCCT GAAAGTGAGCCTGCTGCAGATGGGGACCCAGGGCTGAGCTCCCCGGAGCTCTGTCAGCTCCACCTGTCCA TCTGTCACGAGTGTCTGGAGCTCGAGAACAGTACCATTGACTCGGTGAGGTCTGCGTCTGCGGAGAACAT TCCAGACCTTCCCTGTGATCACAGCGGCGTGGAGGGTGCTGCGGGCGAACTCTGCCCCGAACGGAAAGGG AAGAGAGTCAACATTTCGGGAAAGGCGCCCAATATCTTGCTGTATGTGGGCTCTGGTTCCGAGGAAGCCC TGGGCCGGCTCCAGCAGGTGCGATCTGTCCTGACTGACTGTGTGGACACGGACAGCTACACTCTCTACCA CCTGCTGGAAGACAGCGCTCTGAGGGACCCGTGGTCTGACAACTGCCTCCTGCTAGTTATTGCCAGCAGG GACCCCATCCCCAAAGACATACAGCACAAGTTCATGGCCTATCTTTCTCAGGGAGGGAAGGTGCTGGGCC TGTCTTCCCCCTTCACGTTGGGTGGCTTTCGGGTGACCAGGAGAGATGTGCTACGGAACACAGTCCAGAA CTTGGTTTTCTCTAAGGCTGATGGGACTGAGGTACGGCTCAGTGTCCTGAGCAGCGGCTACGTTTATGAA GAAGGCCCGAGCTTGGGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGATAAAATGATTGTGCACG TGCCTTTTGGAACCCTCGGAGGGGAAGCCGTTCTCTGCCAGGTGCACCTGGAGCTCCCGCCCGGCGCCTC CCTGGTGCAGACTGCAGACGACTTTAACGTGCTCAAATCCAGCAATGTGAGAAGACACGAGGTCCTTAAG GAGATCCTGACTGCCCTCGGTCTGAGCTGTGACGCACCACAGGTTCCTGCCTTAACGCCCCTGTACTTAC TGCTGGCCGCTGAGGAAACCCAGGATCCGTTCATGCAGTGGCTCGGGAGGCACACAGATCCTGAAGGAAT CATAAAATCCAGCAAGCTCTCCCTTCAGTTTGTTTCCTCCTACACATCTGAAGCGGAGATCACCCCGTCT TCCATGCCTGTGGTCACTGACCCAGAAGCCTTTTCATCAGAGCACTTCAGCCTAGAGACCTATCGACAGA ATCTGCAGACCACGCGCCTTGGGAAAGTGATTCTGTTTGCAGAAGTGACCTCCACCACCATGAGTCTCCT GGATGGGTTGATGTTTGAGATGCCGCAGGAAATGGGCTTAATTGCCATTGCAGTTCGGCAGACCCAGGGC AAAGGAAGGGGCCCGAATGCCTGGCTGAGCCCCGTGGGATGTGCTCTTTCCACTCTGCTGGTCTTCATCC CCCTGAGGTCACAACTGGGACAGAGGATTCCATTTGTCCAGCATTTGATGTCCTTGGCTGTGGTGGAGGC AGTGCGGTCTATCCCAGGGTATGAGGATATCAACTTGCGAGTGAAGTGGCCCAACGATATTTATTACAGC GATCTCATGAAGATCGGTGGGGTTCTGGTTAATTCAACACTTATGGGAGAAACATTTTATATCCTCATCG GCTGTGGATTCAATGTGACGAACAGTAATCCTACCATTTGTATCAACGACCTCATCGAAGAACACAATAA GCAGCACGGGGCGGGCCTGAAGCCCCTGCGAGCGGACTGTCTCATAGCCAGAGCCGTGACCGTGCTGGAG AAACTGATCGACAGGTTTCAGGACCAAGGGCCCGACGGCGTTCTTCCCCTCTATTATAAATACTGGGTGC ACGGTGGTCAGCAAGTCCGCCTGGGCAGCACGGAGGGGCCACAGGCATCTATTGTGGGCCTTGATGACTC CGGGTTCCTGCAGGTCCACCAAGAGGATGGTGGGGTTGTGACGGTGCATCCAGATGGCAACTCCTTCGAC ATGCTGAGAAACCTCATCGTGCCCAAGCGACAGTAACACCTTCCTGTGTCCCCAGACAGCTTGGATCTAC TGCAACAGGGAAGCTGCTCCTGGGATCTCCTGGGAGCACTGAGCAGGCGCACTCCGCTTGCATAACTGTT GTCTCTGCCTGCCTAGGGCCTGGGAAGCTAATGCAGAGTTAGAGGCCAGTTGTTAATTCTTCAGGTTGTG GGTTCCCTGCCCCCACCCCCTCCCCACCCCGGGATTTGGGACACATATATGTGGTTTTACTGGGTGTTTG AAGGTGCATCTGGTGAGGATTGACTCACAGGCGAAGAAGGGTCAGTTTAGCCTAGGAGTTTAGTTTGCCT TGTGAGGTATAGAAGTGCTCTTCCCCATGTGGGCTTGGATTTGTGTGTACTGGGAGGGAAATGAGAAGCA AGGGGTTAGGAATAGCGCTGAGATAGTACAAATATATCCCAAGGAGTGAAGGCCTTTAGCCCTCTGCTGT TGAAACAGTGAATGCCTACAGCTCAGGGGCATGCTCACTGCTGTCCCAGAACACACAAGTGTTGACCTGC ACGTAGGGTTGTTGCTGGTTTCCTTTGTCAAGTTGGGCTTCTTGTAGTAGCCTGGAACACAGGACTGAAG GATGGCCACAGAAATGCTGCTAACCAGCGTTATCTCCTTTCCAGGATCAGATGTGTCAGGTTTTAGATGT TTAAGCCATGATGGCAGCTTCCTTAGAGACAAAGGCTTGCCCAGGCTGATGGAGACGTGGGCTCACCCTC TTGCCCACACAAGGGAGGATTCTTGGAGCTTTTAAGCTGATCAGTTGCAGGCTCTGTTAGAATGCCACAT CCCCATAAGCTTGTGTCCCCAAGCAGGGTCGAGTGCAGCACTGTATTGTAAGTCATGACTTTCTGCCCCT AATTTCATAATGTCAGGAAGGAGAGAGAGAGAGACTGACACAAAAATGCCATCAGTTTGGAGAGACGTAT GTGCGTGCAGAGCATGCCTGGTTTTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTTTAAA AGAAGATGTTCAAAACTTGATGTGCTAAAACGTCTCCGTGGAAGAACAAAGGAGAGGAAGCCTTTCTGGG CTTGCGTTTCCAGATGTGCTCCGTGCTTTCCCGTGTACCTCCCGTCTCTGTGGAGCTTGTGAATCATACC CATCGCTGCCCAGCCTGAAAGAACTAGTTCCTGTCTGCCCTCCTGCTTCCTGCCACTTCCACTGAAGTGG GCGGACGGAACAGATCGCTTCGCTTAGAGCAAGATCCTCGTCATAAACGAGTACCATCTTTATCCAGCTT CTGTAGTCAGGGCCAACTCCAGAAACCAGAGGTCTTCGGCATAGAATCCACTCACCCTCAGCCTAGCAGA GTCAGCCCCATTTGAAGCAAAAAATAAAATAAATAAATAACAGGGTGAACTATACCAACCTGACGTGTGT TTGAGGGGCTGTGACACTAAAGGAACTTGAAAGTAACTTTTCTTGCAAATAAGACTTTGACTAATGTTTT TCTCAAAAAAAGAAAAAGATACATATATAAAATTTAGTTCCGCTATTTTTTTTAAATTCATGCGAGTTTA AATAAAACTGTACGCCACCCCAAATTATAAAGTGACAGGCCCAAGAGAGAGAGAGAGAGAGAGAGAGAGA GAGAGAGAGAGAGAGAGCGAGAGCATCTGTAAAATATCATCAAGAATGAAGATCTCCAAGCTGGCTGTTG CAAAGGTTCATTTCTCAGACTTTGTAATATAAGGCTGTTCATCTCTCAGAACCGCCATCATTACATTTGA AATTTCTATGGGGGATTATTTAATGAAAAACATGCTATGTTTTATTTTAAGCTGAAGGCCTATTCTGGAT AGTTCTACTTTGGGGAAAAAAATGTTATCATTTAATTTCCTTTCTGTAAGTTAAAACTAATGAAGTGTGG TCATGTCAGAGCAGACAGAGATGTTCTGGGCATGCTGATTGGCCCTGGGGTAGTTACACTGTTTGGGGGT ATTCTTGCGTATGCAGACAGGACACTCCTTGTCTCGAGAGACAGCACAGCCTGAAGCACTGAAGCTGCAT TCCCCACTGGCCTGACTTGGCCTCACATGAACCAAAGACTTCAGATTTCACATCGTAGAGAGCATTTCTG CCGATGGGGCGTGTAAAGGCTGCCACTGTACCCGCCACATGCGAGGGTATTCACACTCTCAGTGTCCCAG GACCTCCAAGAACTTTACAAGTCTCCTAACCTCGATGCCTCCTAATCTGGAGACGTGGCCACCTAACCCC GACTCTGTATTTGCCGTAAGCAGGTTACTCCTAGTGTTAACCTGCTTGCATATCTACCCCTGGGTGTTGG AAGGCACAGGTAAGCAGGCACTGGGTCTCACACAACTCAGCTGAAGCAGTGAGCGGCTTTGCTACCCTGA CATGCCAGCAACGGGGCAACCTCATTCCTGGTTCATCTCTATGCATCTTTGAAATAAACAAAAATGTAAA CCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30012868 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30012868 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19539 same embryo
	EMAGE:19542 same embryo
	EMAGE:19543 same embryo
	EMAGE:19541 same embryo
	EMAGE:19540 same embryo
	EurExpress:euxassay_004961 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source