EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30011

Ogfr ( MGI:1919325)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006226_01	euxassay_006226_02	euxassay_006226_03	euxassay_006226_04	euxassay_006226_05

euxassay_006226_06	euxassay_006226_07	euxassay_006226_08	euxassay_006226_09	euxassay_006226_10

euxassay_006226_11	euxassay_006226_12	euxassay_006226_13	euxassay_006226_14	euxassay_006226_15

euxassay_006226_16	euxassay_006226_17	euxassay_006226_18	euxassay_006226_19	euxassay_006226_20

euxassay_006226_21	euxassay_006226_22	euxassay_006226_23	euxassay_006226_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T571
Entity Detected:	Ogfr, ( MGI:1919325)
Sequence:	sense strand is shown >T571 TTCCTCGAGCCTGTTGGCCTACTGGAAAGCGGGTCCAGCCTGTCGAGTATGGACGACCCGGAATGCGATT CCACCTGGGAGGACGAGAGCGAGGAGGACGGCGAGGATGGCCAGGCGGATGATACGACCGATGAGGACAC GGGCGACGATGACGGCGACGCGGAGGAGGCACGGCCAAGCCTGTTCCAGTCTAGGATGACAAGGTACCGA AACTGGCGTGCTATGCAGGACATGCAAAGATACCGGCACAACTACCCGGATTTGACAGATCAAGACTGCA ACGGAGACATGTGCAACCTGAGCTTCTACAAAAATGAGATCTGCTTCCAGCCAAATGGCTTTCTCATTGA GGACATTCTTCAGAACTGGAAAGACAACTATGACCTCCTGGAAGAGAATCACTCCTACATCCAGTGGCTG TTTCCCCTGAGGGAACCAGGAGTGAACTGGCATGCCAAGCCCCTCACACTGAAGGAGGTTGAGGCATTTA AAAGCTCCAAGGAAGTCAGAGAGCGTCTTGTCCGGGCCTATGAGCTCATGCTGGGCTTCTATGGGATCCA AC
Notes:	The probe template was PCR amplified from IMAGE:1885007 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1885007 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006226 same experiment
	EMAGE:30927 same embryo
	EMAGE:31817 same embryo
	EMAGE:31784 same embryo
	EMAGE:30870 same embryo
	EMAGE:30843 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source