EMAGE mouse gene expression spatial database

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EMAGE:30870

Msln ( MGI:1888992)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006224_01	euxassay_006224_02	euxassay_006224_03	euxassay_006224_04	euxassay_006224_05

euxassay_006224_06	euxassay_006224_07	euxassay_006224_08	euxassay_006224_09	euxassay_006224_10

euxassay_006224_11	euxassay_006224_12	euxassay_006224_13	euxassay_006224_14	euxassay_006224_15

euxassay_006224_16	euxassay_006224_17	euxassay_006224_18	euxassay_006224_19	euxassay_006224_20

euxassay_006224_21	euxassay_006224_22	euxassay_006224_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 23 moderate expression: see section 19 20 21 22

stomach fundus

strong

regional

strong expression: see section 04 05 06 07 08 09

pericardial cavity

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 moderate expression: see section 19 20

rectum

strong

regional

strong expression: see section 11

midgut

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13

bladder

strong

regional

strong expression: see section 10 11 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T680
Entity Detected:	Msln, ( MGI:1888992)
Sequence:	sense strand is shown >T680 TCCTCGAGNCTGTTGGCCTACTGGACTACTGCAGGTGCTCACAGAAGTTGGACAGCTGCTTTCCCAGGCC CAAAAGCCCCTTCGTTGTCTCCAAACAGTGGTGTGGGTTGAGGGGTGGGACAAGTGGGGACCTCAGAGTC ATTGTTATCCACAGACCATGGCCTTGCCAACAGCTCGACCCCTGCTGGGGTCCTGTGGAAGTCCCATCTG CAGCCGAAGCTTCCTACTGCTTCTCCTTAGTCTTGGGTGGATACCACGTCTGCAGACCCAGACTACAAAG ACAAGCCAGGAGGCCACACTCCTCCATGCTGTGAACGGTGCCGCTGACTTTGCCAGTCTCCCCACAGGCC TCTTTCTTGGCCTCACATGTGAGGAGGTATCTGACCTGAGCATGGAACAAGCCAAGGGGCTGGCTATGGC TGTAAGACAGAAGAACATTACACTCCGGGGACATCAGCTGCGTTGTCTGGCACGTCGCCTTCCTAGGCAC CTCACCGACGAGGAACTGAATGCTCTTCCACTGGACCTGCTGCTCTTCCTCAACCCAG
Notes:	The probe template was PCR amplified from IMAGE:1888713 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1888713 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006224 same experiment
	EMAGE:30927 same embryo
	EMAGE:31817 same embryo
	EMAGE:31784 same embryo
	EMAGE:30011 same embryo
	EMAGE:30843 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source