EMAGE mouse gene expression spatial database

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EMAGE:31784

Casp7 ( MGI:109383)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006180_01	euxassay_006180_02	euxassay_006180_03	euxassay_006180_04	euxassay_006180_05

euxassay_006180_06	euxassay_006180_07	euxassay_006180_08	euxassay_006180_09	euxassay_006180_10

euxassay_006180_11	euxassay_006180_12	euxassay_006180_13	euxassay_006180_14	euxassay_006180_15

euxassay_006180_16	euxassay_006180_17	euxassay_006180_18	euxassay_006180_19	euxassay_006180_20

euxassay_006180_21	euxassay_006180_22	euxassay_006180_23	euxassay_006180_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

choroid invagination

strong

regional

strong expression: see section 08 09 10 11 12 13 16 17 18 19 20 21

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22

thymus primordium

moderate

regional

moderate expression: see section 09 10 11 12 13

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 13 14 15 16 17

diencephalon roof plate

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T552
Entity Detected:	Casp7, ( MGI:109383)
Sequence:	sense strand is shown >T552 TCTCGAGNCTGTTGGCCTACTGGAAAAAAAGCTGCCCTCGACCCTTGCGGAGGACGGACGCTGCCGTGGG CTCCTGGCCGCCGCCGTGGGAACGATGACCGATGATCAGGACTGTGCTGCGGAGCTGGAAAAGGTGGATT CTTCCAGCGAAGACGGAGTTGACGCCAAGCCAGACCGCTCCTCTATCATCTCCTCTATTCTCTTGAAGAA GAAGAGAAATGCCTCTGCGGGCCCCGTCAGGACCGGCCGGGACCGAGTGCCCACTTATCTGTACCGCATG GATTTCCAGAAGATGGGTAAATGCATCATCATAAACAACAAGAACTTCGACAAAGCGACAGGTATGGACG TCCGGAATGGGACGGACAAAGATGCAGGGGCCCTCTTCAAGTGCTTCCAAAACCTGGGTTTTGAAGTAAC CGTCCACAATGACTGCTCTTGTGCAAAGATGCAAGATCTGCTTAGAAAAGCCTCTGAGGAGGACCACAGC AACTCGGCCTGCTTCGCCTGCGTCCTGCTGAGCCACGGGGAAGAGGACCTGATTTACGG
Notes:	The probe template was PCR amplified from IMAGE:1499194 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1499194 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006180 same experiment
	EMAGE:30927 same embryo
	EMAGE:31817 same embryo
	EMAGE:30870 same embryo
	EMAGE:30011 same embryo
	EMAGE:30843 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source