EMAGE mouse gene expression spatial database

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EMAGE:30617

Nalp5 ( MGI:1345193)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_005081_06	euxassay_005081_07	euxassay_005081_08	euxassay_005081_09	euxassay_005081_10

euxassay_005081_11	euxassay_005081_12	euxassay_005081_13	euxassay_005081_14	euxassay_005081_15

euxassay_005081_16	euxassay_005081_17	euxassay_005081_18	euxassay_005081_19	euxassay_005081_20

euxassay_005081_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8046
Entity Detected:	Nalp5, ( MGI:1345193)
Sequence:	sense strand is shown >T8046 AAGAGGAAGTCCGGCTTTCTTTTTGCGGTGGTTACCGGTTTGCAGAAAGTAGGATCTGCACTTAGAGAAA GCACAATGGGTCCTCCAGAAAAAGAAAGTAAAGCAATCTTGAAAGCACGTGGATTGGAAGAGGAACAGAA GTCAGAAAGAAAAATGACTTCTCCAGAAAACGACAGTAAATCAATCCAGAAAGACCAAGGACCAGAGCAG GAGCAGACATCAGAAAGCACAATGGGTCCTCCAGAAAAAGACAGTAAAGCAATCTTGAAAGCACGTGGAT TGGAAGAGGAACAGAAGTCAGAAAGCACAATGTCTCCTTCAGAAAATGTCAGTAGAGCAATCCTGAAAGA CAGTGGATCAGAAGAAGTGGAACAGGCGTCAGAAAGAAAAATGACTTCTCCAGAAAACGACAGTAAATCA ATCCAGAAAGACCAAGGACCAGAGCAGGAGCAGACATCAGAAACCTTACAATCTAAGGAAGAAGATGAAG TGACAGAGGCAGATAAAGATAATGGAGGTGACTTACAAGACTACAAGGCCCATGTGATTGCTAAGTTCGA CACAAGTGTGGATCTACACTATGACAGCCCAGAGATGAAATTATTGTCTGATGCTTTTAAACCATACCAG AAAACCTTCCAGCCTCACACCATTATCCTACATGGAAGACCAGGAGTTGGGAAGTCAGCTTTGGCCAGAA GTATTGTTCTTGGCTGGGCACAGGGTAAACTCTTCCAAAAAATGTCCTTTGTCATCTTCTTCTCTGTTAG AGAAATAAAGTGGACAGAGAAGAGCAGTTTGGCACAGCTGATTGCTAAGGAGTGTCCAGACTCCTGGGAT CTAGTGACAAAGATCATGTCCCAACCAGAAAGACTCTTGTTTGTCATAGATGGCTTGGATGATATGGACT CTGTCCTCCAACATGATGATATGACACTATCCAGAGACTGGAAGGATGAACAGCCCATATACATCCTGAT GTACAGCCTCCTGAGGAAGGCTCTCCTACCTCAGTCCTTTCTCATCATTACCACCAGAAACACAGGCTTA GAAAAACTCAAGTCAATGGTTGTGTCCCCCCTCTATATACTGGTTGAAGGACTGTCTGCATCAAGGAGAT CTCAGCTGGTCCTCGAGAACATCTCCAATGAGTCTGATAGAATACAAGTCTTCCATTCTCTGATAGAAAA TCACCAGCTGTTTGACCAATGCCAGGCCCCCTCTGTGTGCTCCCTGGTCTGTGAGGCTCTACAGCTACAG AAGAAACTGGGAAAGAGATGCACCCTACCCTGCCAGACTCTCACCGGTTTGTATGCCACGTTGGTGTTTC ACCAGCTCACCTTGAAAAGGCCTTCCCAGAGCGCTCTCAGTCAGGAAGAACAGATTACTCTAGTGGGTTT GTGCATGATGGCAGCTGAAGGAGTGTGGACCATGAGGTCGGTATTCTATGATGATGACCTGAAGAACTAT AGCCTAAAGGAGTCTGAGATCTTGGCCCTCTTTCACATGAACATCCTTCTCCAGGTTGGCCACAACAGTG AGCAGTGTTATGTTTTCTCCCACCTCAGCCTGCAGGATTTCTTTGCTGCCTTATATTATGTTTTAGAAGG GCTGGAGGGATGGAATCAGCATTTTTGCTTCATTGAAAACCAAAGGAGCATCATGGAGGTGAAGAGAACT GACGACACTCGCCTCCTCGGGATGAAGCGTTTCTTATTTGGCCTCATGAACAAGGATATCTTGAAGACTC TGGAGGTTCTGTTTGAATATCCCGTGATTCCAACTGTTGAGCAGAAGCTCCAACACTGGGTCTCTCTGAT AGCTCAGCAGGTCAATGGCACCAGCCCAATGGACACCCTGGATGCCTTCTATTGTCTATTTGAGTCTCAG GATGAAGAGTTTGTTGGCGGGGCTCTCAAACGCTTCCAAGAAGTGTGGCTGCTGATTAACCAGAAGATGG ACTTGAAGGTCTCTTCCTACTGTCTCAAGCACTGTCAGAACTTGAAGGCAATCCGGGTGGATATCAGAGA CCTCCTCTCGGTAGATAATACTCTCGAGCTGTGCCCTGTTGTTACTGTCCAGGAGACACAATGTAAGCCC CTCCTCATGGAGTGGTGGGGAAACTTCTGCTCTGTGCTTGGCAGCCTCCGGAACTTGAAGGAGCTGGACT TGGGCGACAGCATCCTGAGTCAACGGGCCATGAAGATACTGTGCCTCGAGCTGCGGAATCAGTCCTGCAG AATACAGAAGCTGACGTTTAAGAGTGCAGAGGTAGTGTCTGGCCTGAAACATCTCTGGAAGCTCCTTTTT AGCAATCAAAACTTAAAGTACCTCAATCTAGGGAACACTCCCATGAAGGATGATGACATGAAGTTAGCCT GCGAAGCGCTGAAACATCCAAAGTGCTCCGTGGAGACTCTGAGGTTGGATTCCTGTGAGTTAACCATCAT TGGTTATGAGATGATCTCCACGCTTCTTATTTCAACCACCAGGCTAAAGTGTCTCAGCCTGGCCAAAAAT AGAGTGGGAGTAAAAAGCATGATATCCCTTGGGAATGCCTTGAGTAGCTCAATGTGTCTACTGCAAAAGT TGATACTGGACAACTGTGGCCTCACACCTGCCAGCTGCCACCTTCTGGTCTCAGCCCTTTTCAGCAACCA GAACTTGACACACCTGTGCCTGTCAAACAACAGCCTGGGGACTGAAGGAGTGCAACAGCTGTGTCAGTTC CTGAGGAATCCAGAATGTGCTCTCCAGCGGCTGATACTGAATCACTGCAACATTGTAGATGATGCTTATG GCTTCCTGGCAATGAGACTTGCAAACAACACAAAGCTGACCCACCTGAGCCTGACCATGAACCCCGTAGG GGATGGTGCAATGAAGCTACTGTGTGAAGCTTTAAAGGAACCTACTTGTTACCTTCAAGAACTGGAACTA GTGGACTGCCAACTCACACAGAACTGCTGCGAGGACCTGGCCTGTATGATCACAACAACCAAGCACTTAA AAAGTTTGGATCTTGGTAACAACGCCCTGGGTGACAAAGGAGTCATAACCCTGTGTGAGGGACTGAAGCA AAGTAGCAGCTCCCTGAGGAGACTTGGGTTGGGGGCATGTAAGTTGACTTCCAATTGCTGTGAGGCATTG TCATTGGCCATCTCTTGCAACCCTCACCTGAACAGCCTAAACCTGGTGAAGAATGACTTCAGTACATCGG GGATGTTGAAGCTGTGCTCTGCGTTCCAATGCCCTGTCTCTAACCTGGGGATAATTGGCCTGTGGAAGCA GGAGTACTATGCCCGAGTGAGAAGACAGCTGGAGGAAGTTGAGTTTGTCAAGCCCCACGTGGTGATTGAT GGTGATTGGTATGCTAGTGATGAAGATGACCGAAACTGGTGGAAAAACTGAAGACATGAGCCCCCTCTCC TTCACGTCCTAGCACTGCAGTATCTGTGAAATGTTTGTCTCACCTTGGAGGATGTAGCAAGAATGAAATA AACACAGCATAAAAAAAAAAAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:30049556 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30049556 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005081 same experiment
	EMAGE:30406 same embryo
	EMAGE:31531 same embryo
	EMAGE:31530 same embryo
	EMAGE:31558 same embryo
	EMAGE:30622 same embryo
	EMAGE:31610 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source