EMAGE mouse gene expression spatial database

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EMAGE:30622

Tnpo1 ( MGI:2681523)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_005089_06	euxassay_005089_07	euxassay_005089_08	euxassay_005089_09	euxassay_005089_10

euxassay_005089_11	euxassay_005089_12	euxassay_005089_13	euxassay_005089_14	euxassay_005089_15

euxassay_005089_16	euxassay_005089_17	euxassay_005089_18	euxassay_005089_19	euxassay_005089_20

euxassay_005089_21	euxassay_005089_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8047
Entity Detected:	Tnpo1, ( MGI:2681523)
Sequence:	sense strand is shown >T8047 AAGAGGTAATCCGGAGCGGTCCGGCCGGGTTTCGGCAAACCAAGATGGAGTATGAGTGGAAACCTGACGA GCAAGGGCTTCAGCAGATCCTGCAGCTGCTCAAGGAGTCCCAGTCCCCAGACACCACCATCCAGAGGACC GTGCAACAAAAACTGGAACAACTCAATCAATATCCAGATTTTAACAACTACTTGATTTTTGTTCTTACAA AATTAAAATCTGAAGATGAGCCCACACGGTCATTGAGCGGTCTGATCTTGAAGAACAATGTGAAAGCTCA TTTTCAGAACTTCCCAAATGGTGTGACAGACTTCATCAAGAGTGAATGTCTAAATAACATTGGGGACTCC TCTCCGTTGATCAGAGCCACTGTCGGTATTTTAATTACAACCATAGCCTCCAAGGGAGAGCTGCAGAATT GGCCTGATCTCTTACCAAAACTCTGTAGCCTGCTGGATTCCGAGGACTACAACACTTGTGAGGGAGCCTT CGGTGCCCTTCAGAAGATATGTGAGGACTCTGCAGAGATTTTAGACAGTGATGTCTTAGATCGCCCTCTC AACATCATGATCCCCAAGTTTTTACAGTTTTTCAAGCACAGCAGTCCCAAAATAAGGTCTCATGCTGTTG CATGTGTCAATCAGTTCATCATCAGTCGGACCCAGGCGCTCATGCTGCACATCGATTCCTTCATTGAGAA CCTCTTTGCACTGGCTGGCGACGAGGAAGCAGAGGTGCGGAAGAACGTGTGCCGGGCGCTTGTGATGTTG CTTGAAGTCCGGATGGATCGCCTGCTTCCTCATATGCACAACATAGTCGAGTACATGCTGCAGAGAACCC AAGACCAAGATGAGAATGTAGCTCTGGAGGCCTGTGAATTCTGGCTGACTTTGGCTGAACAGCCAATATG CAAAGATGTACTTGTAAGGCATCTACCAAAGTTGATTCCTGTGTTAGTGAATGGCATGAAGTACTCAGAT ATAGATATTATCCTGCTTAAGGGTGATGTTGAGGAAGATGAGACCATCCCGGATAGTGAGCAGGATATAC GGCCGCGGTTTCATCGCTCGAGGACAGTGGCTCAGCAGCATGAGGAGGATGGGATTGAGGAGGAAGACGA TGATGATGACGAAATTGATGATGATGACACGATTTCTGACTGGAACCTGAGAAAGTGTTCTGCTGCTGCT CTCGATGTTCTCGCAAATGTTTATCGTGATGAGCTTTTGCCGCACATTCTGCCACTTTTGAAAGAATTGC TTTTCCATCATGAATGGGTTGTGAAAGAATCTGGCATCTTGGTTTTAGGAGCAATTGCTGAAGGTTGTAT GCAAGGCATGATTCCGTACCTGCCCGAGCTCATTCCTCACCTTATTCAGTGCCTTTCTGATAAAAAGGCT CTTGTGCGTTCCATCACCTGCTGGACTCTTAGCCGCTATGCACACTGGGTAGTCAGCCAGCCACCAGATA CGTACCTGAAGCCATTAATGACAGAACTGCTGAAGCGTATCCTCGATAGCAACAAGAGAGTACAGGAAGC AGCTTGCAGTGCCTTCGCTACATTAGAAGAGGAGGCTTGTACAGAGCTCGTCCCTTACCTTGCTTATATA CTCGATACCCTCGTCTTCGCCTTCAGTAAATACCAGCATAAGAACCTGCTCATTCTGTACGACGCCATAG GGACACTGGCAGATTCAGTGGGACATCATTTAAACAAGCCAGAATATATTCAGATGCTAATGCCTCCTTT GATCCAGAAATGGAACATGCTGAAGGATGAAGACAAGGATCTTTTCCCTTTGCTTGAGTGTCTCTCGTCT GTTGCCACAGCCTTGCAGTCTGGCTTCCTTCCATATTGTGAACCTGTATATCAGCGTTGCGTAAACCTAG TTCAGAAGACTCTGGCACAAGCCATGCTAAACAATGCTCAACCAGAACAGTATGAAGCTCCAGATAAAGA TTTTATGATTGTGGCTCTTGACTTACTCAGCGGCCTGGCTGAGGGCCTGGGAGGCAACATTGAGCAGCTG GTGGCCCGCAGTAACATCCTGACGCTGATGTACCAGTGCATGCAGGATAAAATGCCCGAGGTTCGGCAGA GTTCTTTTGCATTGCTAGGTGACCTGACTAAAGCGTGCTTCCAGCATGTTAAGCCTTGTATAGCTGATTT CATGCCAATATTGGGAACCAATCTAAATCCAGAGTTTATTTCAGTCTGCAACAATGCCACCTGGGCGATT GGGGAAATATCAATCCAAATGGGTATAGAGATGCAGCCCTACATCCCTATGGTGTTGCACCAGCTTGTGG AGATCATTAACAGACCCAACACCCCAAAGACGCTGTTGGAGAACACAGCAATAACAATTGGTCGTCTTGG TTACGTTTGTCCTCAAGAGGTGGCCCCCATGCTACAGCAGTTTATAAGACCCTGGTGTACCTCTCTGAGA AACATAAGAGACAATGAAGAAAAAGATTCAGCATTCCGTGGGATTTGTACCATGATCAGTGTGAATCCCA GTGGCGTAATCCAAGATTTTATATTTTTTTGTGATGCTGTTGCATCATGGATTAACCCAAAAGATGATCT CAGAGACATGTTCTGTAAGATCCTTCATGGATTTAAAAACCAAGTTGGGGATGAAAATTGGAGGCGATTC TCTGACCAGTTTCCTCTTCCCTTAAAAGAGCGTCTTGCAGCTTTTTATGGTGTTTAATCTAATACACTTA AGCTGCAGTCCCAAAACTAGGGGTCCGTCAGTCTTGGAGACTATGGGAGCCTCTGCACCCAGGGAAAATG TTACCCTTTACAGGGGGGAAGGGTAAACCAGTAGGGAATACAGTACGATCCCAACCCTACTGGGAGGGGC GGGAGGGAGGTGTTGCCGTCACTGTACTAAGTCGATGTTGGGAAATGTTTTAACATCTGGAGCCTTTGTG GGTGGAAATCTGTCTCCAATTACAACTCTGCACTGGATGTGAAGAAGCAAAAACTATTCCGTCTACTCAC AGAACAGTACTTTCTGGAATATTATGGGGGATTGTACCAAAACAAGAACCACTTAAACAATGCCTAGGGA ATGATAAGGAGGAGGAAAATCCCAGGTCCACAGCAAAGGACACCTAAGAGTGGGAGGCTGCAGCAGTAAG GTGGCTTTTTATCAAAGGTTTTTATATAAATTTCCCACGTTACGGGGCCTTGTTTTGCATGCTGATGAAG AACTACTTAAAACTAACAGTTAAAATCAGCTTGCCTCCCCTAGTAGAAGCAGGTTCTTGGAAGTTACAAT TTAAGGTACCCCCAAAAAGTTGGAAATAAAAGGAAACAAATTTGAACAACGAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30059046 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30059046 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005089 same experiment
	EMAGE:30406 same embryo
	EMAGE:30617 same embryo
	EMAGE:31531 same embryo
	EMAGE:31530 same embryo
	EMAGE:31558 same embryo
	EMAGE:31610 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source