EMAGE mouse gene expression spatial database

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EMAGE:31558

Anxa13 ( MGI:1917037)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005039_01	euxassay_005039_02	euxassay_005039_03	euxassay_005039_04	euxassay_005039_05

euxassay_005039_06	euxassay_005039_07	euxassay_005039_08	euxassay_005039_09	euxassay_005039_10

euxassay_005039_11	euxassay_005039_12	euxassay_005039_13	euxassay_005039_14	euxassay_005039_15

euxassay_005039_16	euxassay_005039_17	euxassay_005039_18	euxassay_005039_19	euxassay_005039_20

euxassay_005039_21	euxassay_005039_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

weak

regional

weak expression: see section 06 07 08 09 10 13

hindgut

strong

regional

strong expression: see section 11

kidney calyx

strong

regional

strong expression: see section 05 06 07 13 14 15 16

rectum

strong

regional

strong expression: see section 11

midgut

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

midgut loop

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7478
Entity Detected:	Anxa13, ( MGI:1917037)
Sequence:	sense strand is shown >T7478 CCAGAGAAGAGGAAATGGGGAATCGTCATGCCAAAGAGAGAAGCCATCACCACGGCTTTGATGCAGACCG AGATGCCAAAAAGCTGTACAAAGCCTGCAAAGGAATGGGAACGGATGAAGCAGCCATCATTGAAGTCTTA TCCAGCAGGACATCGGAGGAGAGGCAACAGATAAAGCAGAAGTACAAGGAGAAGTATGGCAAGGACTTAG AGGAGGTGCTTAACAGTGAACTGAGTGGAAACTTCAAGAAGACAGCTTTGGCCCTTCTGGATCGGCCCAA TGAGTACGCCGCACGGCAGCTGCAGAAAGCTATGAAGGGCGTAGGCACCGACGAGGCCATGCTCATAGAG ATCCTATGCACACGAAGCAACAAGGAAATTGTCGCCATCAAAGAAGCTTACCAAAGGCTATTTGGCAGGA GCCTTGAATCTGATGTCAAAGAAGACACAAGTGGAAACCTGAGAAAGATTCTGGTGTCCCTGTTACAGGC CAGTCGTGATGAAGAAGACACTGTGGACAAGGAATTGGCTGGTCAGGATGCCAAAGATTTGTATGATGCA GGCGAAGGTCGCTGGGGCACTGACGAACTTGCCTTCAATGAAGTCCTGGCCAAGAGGAGCTACAAGCAGT TGCGCGCCACCTTTCAAGCTTATCAGATTCTCATTGGCAAAGACATGGAAGAAACCATTGAAGAAGAGAC ATCGGGAGACTTGAAGAAGGCCTATTTAACTATAGTGAGATGTGCCCAGGACCTTGAGGGCTATTTTGCT GACCTACTATACAAGGCCATGAAGGGTATGGGGACAGATGAGGAGACATTAATTCGCATCATTGTGACCA GAGCAGAGGTGGACCTGCAGGGGATAAAGGCCAAGTTCCAGGAGAAGTATCAGAAGTCTCTCTCTGACAT GGTTCACTCAGATACCTCTGGGGACTTCCGGAAACTGCTGGTAGCTCTCTTGCACTGATCCAAGCCTGAG GAGAAACCCCCATAGCAGGATTCTTCCTCGGAACAAATCAAATTTGGAAATGGGTGAAAACCCTTCACCC CCTGGATTGTTTATTCATGGATTTAAAAGGAGCCCCAAGGCTAAATTGTCGAAGTCGGGAGCAGCCTCCT CCAAGGGCTTGAACAGTTCACCTTGAATGCTGGCTTAGCAGGAACCTGAACTGCCTGCCTTCCAGTCTCC CTTTAGCATTTGAAGCGAATGCTTTCTAAGCACAAATGGAATCAGCAGTTGGTGGGGGGAAGAACCCGAA CATTTATAATAAAATGTTTAGAAAATTATATGCCTCCCAGATGTACAGAGCCAAAGCAGAGGTTAAATGG CTAATGCGGCGTAAAGACAAGATTAAACGGTTTGCTCATTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4236501 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4236501 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005039 same experiment
	EMAGE:30406 same embryo
	EMAGE:30617 same embryo
	EMAGE:31531 same embryo
	EMAGE:31530 same embryo
	EMAGE:30622 same embryo
	EMAGE:31610 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source