EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30650

Ipo4 ( MGI:1923001)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_012112_01	euxassay_012112_02	euxassay_012112_03	euxassay_012112_04	euxassay_012112_05

euxassay_012112_06	euxassay_012112_07	euxassay_012112_08	euxassay_012112_09	euxassay_012112_10

euxassay_012112_11	euxassay_012112_12	euxassay_012112_13	euxassay_012112_14	euxassay_012112_15

euxassay_012112_16	euxassay_012112_17	euxassay_012112_18	euxassay_012112_19	euxassay_012112_20

euxassay_012112_21	euxassay_012112_22	euxassay_012112_23	euxassay_012112_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30744
Entity Detected:	Ipo4, ( MGI:1923001)
Sequence:	sense strand is shown >T30744 GGCCTGCTATGCTCTGGAGAATTTTGTGGAGAACCTAGGGCCCAAAGTGCAGCCCTACCTTCCGGAGCTT ATGGAATGTATGCTGCAGCCATTGAAGAACCCCAGCAAAGCCCGGACCAAGGAGCTAGCTGTGAGCGCCA TAGGGGCCATTGCCACAGCGGCCCAGGATTCCCTGCTGCCCTACTTCCCTACCATCATGGATCTTCTCCG GGAGTTCTTGTTGACAGGCCACGAAGACTTTCATCTTGTGCAGATCCAGAGCCTGGAGACCCTGGGAGTG CTAGCACGAGCCCTGGGAGAGTCCATGAAGCCCCTGGCTGAGGAATGCTGTCAGCTTGGGCTAGGGTTAT GCATCCATATAGATGACCCTGACGTGAGGCGCTGCACGTACAGCCTGTTTGCAGCCTTATCAGGGTTAAT GGGAGAGGGCTTGGGACCCTACCTGCCTCAGATTACCACGCTCATGCTGCTGTCGCTGCGCTCCACTGAG GGCATTGTGCCACAGTATGACGGGATCAGCTCCTTCCTGCTGTTTGATGACGACAGCGAAGCAGAAGAGG AGGAGGAGCTCATGGATGAAGATATGGAAGAGGAGGGGGATGACTCTGAGATCTCGGGGTATAGTGTGGA GAATGCCTTCTTTGATGAGAAAGAAGACACCTGCACCGCCCTGGGGGAGATCTCCATGAACACCTGTGTG GCTTTTCTCCCATTCATGGATGCCACCTTCGATGAAGTATACAAACTGCTGGAGTGCCCTCATATGAATG TGCGGAAGTCAGCTTACGAAGCGCTGGGACAGTTCTGCTGTGCACTGCATAAGGCCTCTCAGAGAAGCTC CTCAGATCCCAGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:2649233), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 13139. Forward Primer - name:013139_F_IRAV01-03_G11_Ipo4, sequence:GGCCTGCTATGCTCTGGA; Reverse Primer - name:013139_R_SP6_IRAV01-03_G11_Ipo4, sequence:TGCTGGGATCTGAGGAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_012112 same experiment
	EMAGE:31990 same embryo
	EMAGE:30652 same embryo
	EMAGE:30651 same embryo
	EMAGE:32008 same embryo
	EMAGE:31986 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source