EMAGE mouse gene expression spatial database

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EMAGE:32008

Aco2 aconitase 2, mitochondrial ( MGI:87880)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_012108_01	euxassay_012108_02	euxassay_012108_03	euxassay_012108_04	euxassay_012108_05

euxassay_012108_06	euxassay_012108_07	euxassay_012108_08	euxassay_012108_09	euxassay_012108_10

euxassay_012108_11	euxassay_012108_12	euxassay_012108_13	euxassay_012108_14	euxassay_012108_15

euxassay_012108_16	euxassay_012108_17	euxassay_012108_18	euxassay_012108_19	euxassay_012108_20

euxassay_012108_21	euxassay_012108_22	euxassay_012108_23	euxassay_012108_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 07 08 09 10 11 12 13 17 moderate expression: see section 14 15 16

heart atrium

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

ventral grey horn

strong

regional

strong expression: see section 13 14 15 16 17 18

heart ventricle

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

olfactory cortex mantle layer

strong

regional

strong expression: see section 10 11 12 13 14 17 18 19

metanephros

strong

regional

strong expression: see section 08 09 10 11 12 20 21 22 23 24

axial musculature

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 19 20 21 22 23 24

midgut

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30745
Entity Detected:	Aco2, aconitase 2, mitochondrial ( MGI:87880)
Sequence:	sense strand is shown >T30745 GGACATGGGACGATCAGCAGCTGTGGCTAAGCAGGCATTGGCCCACGGACTCAAGTGCAAGTCCCAGTTC ACCATCACCCCAGGCTCTGAGCAGATCCGTGCCACTATTGAGCGGGATGGCTATGCACAGATCTTAAGGG ATGTGGGTGGAATCGTTTTGGCCAATGCCTGTGGCCCTTGCATCGGCCAGTGGGACAGAAAAGACATCAA GAAGGGGGAGAAGAATACAATCGTCACCTCTTACAACAGGAACTTCACAGGCCGTAATGATGCAAACCCT GAGACCCATGCCTTTGTCACATCCCCAGAGATTGTCACAGCTCTGGCCATTGCAGGAACCCTGAAGTTCA ATCCAGAAACTGACTTCTTAACAGGCAAGGACGGCAAGAAGTTCAAGTTGGAGGCTCCAGATGCAGACGA GCTTCCCCGATCGGACTTTGATCCAGGGCAGGACACATACCAGCACCCCCCTAAGGACAGCAGTGGGCAG CGGGTGGACGTGAGCCCTACCAGCCAGCGCCTGCAGCTCCTGGAGCCTTTCGACAAGTGGGACGGCAAAG ACCTCGAGGACCTGCAAATCCTCATCAAGGTCAAAGGGAAGTGCACCACAGACCACATTTCTGCTGCCGG CCCCTGGCTCAAGTTCCGTGGGCATCTGGATAACATCTCTAACAACCTGCTCATCGGTGCCATCAACATC GAAAATGGCAAAGCCAACTCTGTACGCAATGCTGTCACCCAGGAGTTTGGCCCTGTACCTGACACTGCTC GCTACTACAAGAAACATGGCATCAGGTGGGTGGTGATTGGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3158239), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 34927. Forward Primer - name:034927_F_IRAV01-03_G12_Aco2, sequence:GGACATGGGACGATCAGC; Reverse Primer - name:034927_R_SP6_IRAV01-03_G12_Aco2, sequence:CTCCAATCACCACCCAC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_012108 same experiment
	EMAGE:31990 same embryo
	EMAGE:30652 same embryo
	EMAGE:30651 same embryo
	EMAGE:30650 same embryo
	EMAGE:31986 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source