EMAGE mouse gene expression spatial database

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EMAGE:30925

Ephb6 Eph receptor B6 ( MGI:1096338)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005595_01	euxassay_005595_02	euxassay_005595_03	euxassay_005595_04	euxassay_005595_05

euxassay_005595_06	euxassay_005595_07	euxassay_005595_08	euxassay_005595_09	euxassay_005595_10

euxassay_005595_11	euxassay_005595_12	euxassay_005595_13	euxassay_005595_14	euxassay_005595_15

euxassay_005595_16	euxassay_005595_17	euxassay_005595_18	euxassay_005595_19	euxassay_005595_20

euxassay_005595_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

peritoneal component

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 17 18 19

pericardial cavity

moderate

regional

moderate expression: see section 02 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

kidney calyx

strong

regional

strong expression: see section 07 08 14 15

pleural cavity

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4374
Entity Detected:	Ephb6, Eph receptor B6 ( MGI:1096338)
Sequence:	sense strand is shown >T4374 ATAACTCCTACCAGACAAGAAGGCAAGCCTGGCTGTAGACACCAGGTCTTCTGAAAGCCATAGCAGTGCC TCAGAACAGGGCAGTGGCCGAGGCAATGGTGGGAGTGCCGAAGAGACCGCATGGCTACTGAGGGCACGAC TGGCTCAGGGAGCAGAGTGGTGGCGGGCATGGTGTGCAGTCTGTGGCTCCTGGTTCTAGGGTCATCAGTT CTAGCTCTGGAAGAGGTGCTGCTGGATACCACAGGAGAGACCTCTGAGATTGGCTGGCTCACCTACCCGC CAGGTGGGTGGGATGAGGTGAGTGTTCTAGATGACCAGCGCCGTCTGACTCGGACCTTCGAAGCATGCCA CGTTGCAGGGCTCCCTCCTGGTTCTGGCCAGGACAATTGGCTGCAGACACACTTTGTGGAACGGCGAGGG GCCCAGAGGGCACACATCCGCCTTCACTTCTCTGTACGAGCCTGTTCCAGCCTGGGTGTGAGTGGGGGTA CCTGCCGAGAGACCTTCACCCTTTACTACCGGCAGGCTGATGAGCCAGACGGCCCCGACAGTATTGCGGC CTGGCACCTCAAACGCTGGACCAAAGTGGACACAATTGCCGCAGATGAGAGCTTCCCTGCCTCCTCTTCC TCTTCCTCATGGGCTGTGGGACCCCACAGGACCGGTCAGCGGGTGGGACTGCAGCTGAATGTCAAAGAGC GCAGCTTTGGTCCTCTCACTCAGCGTGGCTTCTATGTGGCCTTCCAGGACACAGGGGCCTGCCTGGCCCT TGTGGCAGTCAAGCTCTTCTCCTACACCTGTCCCTCCGTCCTGCGTGCCTTTGCCTCTTTTCCTGAGACA CAGGCCAGTGGAGCTGGAGGGGCTTCCTTAGTGGCAGCTGTGGGCACCTGTGTAGCTCATGCAGAACCAG AGGAGGATGGAGTAGGAGGCCAGGCAGGGGGCAGCCCCCCAAGGTTACACTGTAATGGCGAAGGCAGGTG GATGGTAGCTGTGGGAGGCTGCCGCTGCCAGCCTGGACACCAGCCTGCTCGTGGAGACAAGCTCTGCCAA GCCTGCCCTGAGGGATCCTATAAGGCCCTGGCTGGGAATGTTCCCTGTTCACCGTGTCCAGCCCGCAGCC ACTCCCCTGACCCTGCAGCCCCAGTTTGCCCATGTCTCCAAGGCTTCTACAGGGCCAGTTCGGACCCACC AGAGGCTCCCTGCACTGGTCCTCCATCGGCTCCCCGGGAGCTATGGTTTGAGGTGCAAGGCTCGGCACTC ATGCTGCACTGGCGTCTGCCTCAGGAGCTGGGTGGACGAGGGGATCTGCTCTTCAATGTTGTGTGCAAGG AGTGTGGAGGTCACGGAGAGCCCAGCAGTGGGGGGATGTGTCGCCGCTGCAGGGACGAGGTGCATTTCGA CCCCCGCCAGAGGGGACTGACTGAGAGTCGAGTGTTAGTTGGGGGGCTCCGAGCACACGTGCCATACATC TTGGAAGTGCAGGCTGTCAATGGGGTGTCTGAGCTCAGCCCTGACCCTCCCCAGGCAGCAGCCATCAATG TCAGCACCAGCCACGAAGTCCCTTCTGCAGTCCCTGTGATGCACCAGGTGAGCCGGGCAGCCAACAGCAT CACAGTATCCTGGCCACAGCCTGAGCAAACAAATGGGAACATCCTCGACTACCAGCTGCGCTACTATGAC CAGGCAGAAGATGAATCCCACTCCTTCACCATGACCAGTGAGACCAACACTGCCACTGTGACACGGTTGA CCCCTGGCCACATCTATGGCTTCCAGGTGCGGGCACGGACTGCAGCTGGCCACGGCCCCTATGGGGGCAA AGTCTATTTTCAGACATTGCCTCAAGGTGAGCTGTCCTCCCAGCTTCCAGAGAAGCTGTCCTTGGTGATT GGTTCCATCCTGGGGGCCTTGGCCTTCCTTCTACTGGCAGCCATCACTGTACTGGCTGTCATCTTCCAGC GGAAGCGCCGTGGGACCGGCTACACAGAACAACTGCAACAATACAGCAGCCCAGGACTTGGAGTGAAGTA TTACATTGACCCTTCTACATACGATGACCCCTGCCAGGCCATCAGAGAGCTTGCTCGAGAGGTTGACCCT ACATATATCAAGATTGAAGAAGTCATTGGGGCAGGTTCCTTTGGAGAAGTGCGTCGGGGCCGACTGCAAC CCCGGGGAAGGAGGGAACAAGCTGTGGCTATCCAGGCCTTGTGGGCAGGGGGTGCTGAGAGCCTGAAGAT GACCTTTCTGGGTCGGGCAGCACTGCTGGGCCAGTTCCAGCACCCCAATATCCTGCGGCTGGAAGGTGTA GTCACTAAGAGTCGGCCTGTCATGGTGCTGACAGAACTCATGGAACTTGGTCCCCTGGACAGCTTTCTTA GGCAGCGAGAGGGCCAGTTCAGTAGCCTGCAGCTGGTGGCCATGCAGCGGGGTGTGGCTGCTGCCATGCA GTACTTGTCCAGCTTTGCCTTTGTTCACCGAGCACTCTCGGCCCGAAGTGTGCTAGTGAATAGCCACCTG GTGTGTAAGGTGGCACGTCTCGGCCACAGTCCTCAGGGTTCAAGTTCCTTGCTTCGCTGGGCAGCCCCAG AGGTCATTACACATGGAAAGTATACAACATCTAGCGATGTCTGGAGCTTTGGCATACTCATGTGGGAAGT GATGAGCTATGGAGAACGGCCCTACTGGGACATGAACGAGCAGGAGGTACTAAATGCAATAGAGCAAGAG TTCCGGCTGCCCCCACCTCCAGGGTGCCCCCCTGGACTACATCTACTGATGCTAGACACTTGGCAGAAGG ACCGTGCCCGTCGGCCTCATTTTGACCAGCTGGTAGCTGCATTTGACAAGATGATTCGCAAGCCTGATAC TCTCCAGGCTGAAGGGGGCTCCGGGGACAGGCCTTCCCAGGCTCTTCTGAACCCTGTGGCCTTGGACTTC CCTTGCCTGGACTCTCCCCAGGCCTGGCTTTCAGCCATTGGACTAGAGTGCTACCAGGACAACTTCTCCA AGTTTGGTCTTTCCACCTTCAGTGATGTGGCTCAGCTCAGCCTGGAAGACCTGCCAGGCCTGGGCATCAC CCTAGCTGGCCACCAGAAGAAACTTCTGCACAACATTCAGCTCCTCCAGCAGCACCTGAGGCAGCCAGGC TCAGTGGAAGTGTAAAGCCTGCTCAGGAGTCTGTGAGCCGCCGATGCCCCAACCCAGTCCTGGCCATAGG GTGGAGAAGGGGCGTGTGAGACTGAGTCAGGCTCTACTCCAGCTTCCATGCCTGTCACATTAAACCCAGC CCTCTGGACATTGCACCCCCAAATCCTGCTCTTCCAACAGCCTCCCCCATATAAAGGGAAAGAAGGGAAT TTAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4911959 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4911959 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005595 same experiment
	EMAGE:31351 same embryo
	EMAGE:31906 same embryo
	EMAGE:29254 same embryo
	EMAGE:31961 same embryo
	EMAGE:30907 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source