EMAGE mouse gene expression spatial database

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EMAGE:31351

2810410C14Rik ( MGI:1919657)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005589_01	euxassay_005589_02	euxassay_005589_03	euxassay_005589_04	euxassay_005589_05

euxassay_005589_06	euxassay_005589_07	euxassay_005589_08	euxassay_005589_09	euxassay_005589_10

euxassay_005589_11	euxassay_005589_12	euxassay_005589_13	euxassay_005589_14	euxassay_005589_15

euxassay_005589_16	euxassay_005589_17	euxassay_005589_18	euxassay_005589_19	euxassay_005589_20

euxassay_005589_21	euxassay_005589_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 13 14 15 16

vagus x ganglion

moderate

regional

moderate expression: see section 08 14 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 14 15 16 17 18 19 20

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 15 17

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 16

trigeminal v nerve

moderate

regional

moderate expression: see section 08 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4963
Entity Detected:	2810410C14Rik, ( MGI:1919657)
Sequence:	sense strand is shown >T4963 AACCTGACTGCCACTGGGTATCATGGCCTACTCATCAGCTACAAGACCTGAGACCTCCCTTTTCTCTCTG AAGCCTGTAAGAGGAACCCCCACTTAGCTTCATGAGCCTGAGCCTTCTTTGGAACTCCCGCCATAATATC TGCCTCTGCTGTTAATAACTCTGTTCTCAGAAGGACAGTCTACCCCAGCATTCCTCCTGAAATCCAAAAC CTGAGACGTTTCAGGCCTCAGCGTCCCCATATGTGATACAAGGATGTTAGACTGGATCGTCTCTAAGGCG CCCTCAGTTTTTCAACTCTCTAGCTCTGGAACTGACTAGATCTGAAGGATGAGCAGGTGTTTTGTACAGG GGATACACTATGAACAATAAAGACCTTGAGAGCTGTTCTGCCAAGCTGAGTCCAGAGACTTTCCTGGCCT TCCACATCAGCACCATGGAGAAGGTCACCAAGGACACCTATCTTGTCCGGTTTACACTACCCGGGAACAG TCGGCTCGGCCTGCGTCCTGGACAGCACCTCATCCTACGAGGTGTAGTAGATGGCTTAGAGATTCAGAGA GCCTACACTCCCATCAGCCCTGTCACTGCAGAAGGATACTTTGACGTTTTAATCAAGTGCTACCGGACTG GGCTGATGTCCCAGTATGTGGAGTCATGGAGAACAGGAGACACAGCCTTCTGGCGAGGGCCTTTCGGAAG CTTCTTATATGAACCAAAGAAGTACGGCGAGCTCCTCATGCTAGCGGCTGGCACAGGCCTGGCCCCCATG GTGCCAATCCTGCAGAGCATCACAGACGACGAAGATGATGAGACCTTTGTCACCTTGGTCGGCTGCTTCA AGACCTTCGAGGGCATCTACCTGAAGACCTTCTTCCAGGAACAGGCCAGGTTCTGGAACGTGCAAACCTT CTTTGTCCTCAGTCAGGAGGTCTCCCCAGAACAGCTTCCCTGGAGCTACCGTGACAAGACCCATTTTGGC CGCCTGGGCCAGGAGCTGGTTGCTGAACTGGTGGCCTGCTGCCGGAGAAAGCCGTTTACCTTAGTCTGTG GCTCACCTGCATTTAATGAGGACATGGCTAGGTGTTTGCTGTCTGCTGGCCTGACTGAGGACTCCTACTT CCTCTTCTAGCCCTGGCCTTGGGCCTTAATCAGGGGTACAGAGAGGAGCCCAGATCAGAGTCAGAAGACA TGGAGTCCAGGCCTGGCCCTGCTGCTGACTTACCCAGAGACCTTGGGCAAATGTCATCACCATGGAACTT CCCTTCCTGTCTGCTGCGTCCTGCTGTATGGAGGGAGGCCAGCAGTTGTCAGGGAGTCAGGGAAGACTGC TCCAAGGGAGCCAAGCAAGTGGAGTCCTACTGAGCTCAGGCTGGTGGGGGTTGGGTAGGACAAGGGGTGA GGAGTCTCTTGTCCTCCCAGAGCCCATCAGTGCTGGGAAAATGACTTGGGGAAGCTCAGAGCTCTGGGAG GACCTTAACTTCTGTGCCTCCTGTGGGGTGGACCTGACTTGCTGAAGGCCTGGAGTCTAAGACCTGACCA TGTTCTCAGATCAAGGCACTGCCCTGAATCATCTCTGTATCCATGACAAGTCTTTCCTGCCATGAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5136046 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5136046 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005589 same experiment
	EMAGE:31906 same embryo
	EMAGE:29254 same embryo
	EMAGE:30925 same embryo
	EMAGE:31961 same embryo
	EMAGE:30907 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source