EMAGE mouse gene expression spatial database

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EMAGE:31906

Rsnl2 ( MGI:1919100)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005612_01	euxassay_005612_02	euxassay_005612_03	euxassay_005612_04	euxassay_005612_05

euxassay_005612_06	euxassay_005612_07	euxassay_005612_08	euxassay_005612_09	euxassay_005612_10

euxassay_005612_11	euxassay_005612_12	euxassay_005612_13	euxassay_005612_14	euxassay_005612_15

euxassay_005612_16	euxassay_005612_17	euxassay_005612_18	euxassay_005612_19	euxassay_005612_20

euxassay_005612_21	euxassay_005612_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

urethra of male

strong

regional

strong expression: see section 11 moderate expression: see section 10 12

trunk mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 15 16 17 18 19

bladder

moderate

regional

moderate expression: see section 10

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4402
Entity Detected:	Rsnl2, ( MGI:1919100)
Sequence:	sense strand is shown >T4402 ATTAAAGACCAACACCTTTCTGCAGCATGGGGAAGAACTAGAAATGCCAGCGCCCACATCTGCTAGGGTT TCAATGTCACTTCTTTCTGGTTATGAGAAGATGGAAGAGATGAGAAGTATGAGGTGGTTTTCTAGAAGAT GACCATAGAGGACCTCCCGGATATTCCATTGGAAGGAAGCTCTCTGATTGGAAGATACCCATTTTTATTC ACAGGCTCAGATACATCTGTTATCTTCTCCATTTCTGCAGCACCGATGCCTTCGGACTGCGAGTTTTCTT TCTTTGACCCTAACGATGCATCATGCCAGGAAATTCTCTTCGACCCCAAAACTTCAGTCTCAGAATTATT TGCTATTTTAAGACAGTGGGTTCCTCAGGTCCAGCAGAACATAGACATTATTGGAAATGAGATTCTTAAG AGAGGCTGCAATGTGAACGATAGAGACGGACTGACGGACATGACTCTGCTACATTACACTTGCAAGTCTG GGGCTCATGGCATCGGTGATATTGAGACAGCTGTAAAATTTGCTGCTCAACTTATAGACCTGGGAGCAGA CGCGAGTTTGCGGAGTCGCTGGACGAACATGAACGCTTTGCATTACGCTTCGTACTTTGATGTCCCAGAG CTTATCAGGGTGATTTTGAAGACATCGAAACCAAAAGATGTGGATGCCACTTGCAGCGACTTTAATTTTG GGACAGCCCTGCACATTGCAGCACATAACTTGTGTGCAGGAGCCGTGAAAACCTTGCTGGAGCTGGGAGC GAACCCTGCCTTTAGGAATGACAAAGGGCAGATCCCTGCAGAAGTTGTTCCAGACCCAGTGGACATGCCT CTGGAGATGGCCGATGCTGCAGCGATCGCGAAGGAAATCAAACAGATGCTCTTGGATGCGGTGCCTCTGC CCTGTACCAGCGCTAAGGCCGTGCTTCCAAACTCCGATCACACCACCAGCAGGGCCATGCTGACATCTCT CGGCCTGAAGTTGGGGGACCGTGTTGTCATCGCAGGGCAGAAGGTTGGAACATTAAGATTTTGTGGAACA ACGGAGTTTGCAAGCGGGCAGTGGGCTGGCATTGAGCTGGACGAGCCAGAAGGGAAGAACAACGGCAGTG TTGGGAGAGTCCAGTACTTCAAGTGTGCCCCCAAATACGGTATTTTTGCTCCCCTTTCAAAGATAAGTAA ATTAAAAGACGGGAGGAAGACCACCACACACACTCCTTCCACGAGAGCCACCCCACACGCCCGATCCCAG AAGGTCGATGTAGCCCACTTCACATCCAGAGTCAATTCCGGGTTGACGACATCTAAAAAGGAGACTGCTT CTGAATCCACACTGACATTGCCTCCCAGTGAAGAACCGAAAACTGTAGCGGAAAATGACGCTGCCCAGCC TGGGTCCATGAGCAGTTCCTCCTCCTCCTCCTCCCTGGACCACAAGCAGAGCTACCCGAAGAAGCTGACC ACAAGCAGCGGTGGCAAGAAGACTCTGAGCAAAAGCCCCTCTCTCCCGTCCAGAGCCAGTGCTGGTTTGA AATCTTCAGCCACATCTGCAGCAAATAACAGTCACCATGAAGGAGCGCTCCACCTTGGAGAAAGGGTGCT GGTCGTAGGCCAGCGAGTAGGCACCATTAAGTTCTTTGGAACAACAAACTTTGCTCCAGGGTATTGGTAC GGCATAGAGCTGGAGAAGCCCCACGGGAAGAATGATGGTTCGGTTGGAGGTGTACAGTATTTTAGTTGCT CTCCGAGATATGGAATATTTGCGCCCCCGTCCAGGGTACAGAGGCTATCAGATTCCCTGGATACGCTTTC AGAAATATCTTCAAATAAACAGAATCACTCTTATCCTGGTTTTAGGAGAAGTTTCAGCACCACCTCTGCT TCTTCTCAAAAAGAGATTAACAGGAGAAATGCTTTTGCCAAGACCAAGACCACCTTGCGCCGCAGCTGGA GCAGTTCCACCACGGCAGGTGGTCTGGAGGGGACCGTGAAGCTCCACGAGGGGTCTCAGGTCCTGCTCAC AAGCTCTAACGAAATGGCCACGGTTAGGTACGTGGGTCCCACGGACTTTGCCTCGGGCATCTGGCTTGGA CTAGAGCTCCGAAGTGCCAAGGGGAAAAATGACGGTGCTGTGGGTGACAAGCGCTATTTCACCTGTAAGC CGAACTACGGAGTGCTGGTTAGACCAAGCCGAGTGACCTACCGCGGGATTAGTGGGTCAAAGCTCATTGA TGAGAATTGTTGAGTTAACCGCCTGAAGCGCTGAGCTTCTCTGAATATATATATATATATGTGCTAGATG AAAAATAAGGCCTATGACAAAGGGTTTACTTTACTTAGCTATTATGTGACAGCTGAAAATAGAGCGACCT TCATAAAAATATCCAAAGGAACACAGGTATGAACTTTGAGGGTCATGGTTCTTTTGAAAATAATCAGAAT GAGCACTTTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4920788 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4920788 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005612 same experiment
	EMAGE:31351 same embryo
	EMAGE:29254 same embryo
	EMAGE:30925 same embryo
	EMAGE:31961 same embryo
	EMAGE:30907 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source