EMAGE mouse gene expression spatial database

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EMAGE:30965

Zfp131 zinc finger protein 131 ( MGI:1919715)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002507_01	euxassay_002507_02	euxassay_002507_03	euxassay_002507_04	euxassay_002507_05

euxassay_002507_06	euxassay_002507_07	euxassay_002507_08	euxassay_002507_09	euxassay_002507_10

euxassay_002507_11	euxassay_002507_12	euxassay_002507_13	euxassay_002507_14	euxassay_002507_15

euxassay_002507_16	euxassay_002507_17	euxassay_002507_18	euxassay_002507_19	euxassay_002507_20

euxassay_002507_21	euxassay_002507_22	euxassay_002507_23	euxassay_002507_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 17 18

axial skeleton tail region

moderate

regional

moderate expression: see section 07 08 09 10 11

axial musculature

moderate

regional

moderate expression: see section 04 05 06 07 08 20 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5094
Entity Detected:	Zfp131, zinc finger protein 131 ( MGI:1919715)
Sequence:	sense strand is shown >T5094 GTGGCCAGGCTTCGCAGGGATTGGCGCTCCGGGTGCGTCCGCGTCCGGCGTGGTGACGCAGCACGTCGAC AGCACCCCGACGGCCATGGAGGCTGAAGAGACGATGGAATGCCTTCAGGAGTTTCCTGAACATCATAAAA TGATCTTGGACCGATTGAATGAACAACGGGAGCAGGACCGGTTTACGGACATCACCCTGATTGTCGACGG ACACCATTTTAAGGCCCACAAGGCTGTTTTGGCTGCCTGCAGTAAGTTCTTCTACAAATTCTTCCAGGAG TTTACTCAAGAGCCTTTGGTTGAAATTGAAGGTGTTAGTAAAATGGCTTTTCGCCACTTAATTGAGTTCA CATACACAGCAAAACTAATGATACAAGGGGAAGAAGAAGCCAATGATGTGTGGAAAGCAGCAGAGTTTCT ACAAATGCTGGAAGCTATTAAAGCACTTGAAGTCAGGAACAAAGAAAACTCAGCTCCATTAGAGGAAAAC ACTACAGGAAAAAATGAGGCAAAAAAAAGAAAGATTGCAGAAACTTCAAATGTTATCACTGAATCATTAC CATCTGCAGAATCAGAACCTGTGGAAATTGAGGTGGAGATTGCTGAGGGCACAATTGAAGTAGAAGATGA AGGCATCGAAGCTTTGGAGGAAATGGCTTCTGCCAAGCAGTCTATAAAGTACATACAGAGCACAGGCTCC TCCGATGATTCCGCTCTGGCGTTGTTGGCAGATATCACCAGCAAGTACCGTCAAGGTGAAAGCAAAGGAC AGATTAGCGAAGATGACTGTGCATCTGACCCCATAAGCAAACAGGTAGAAGGTATTGAAATTGTGGAACT TCAGCTGTCACATGTGAAGGACTTGTTCCATTGTGAGAAATGTAACCGTTCATTTAAATTGTTTTACCAT TTTAAGGAACACATGAAATCACACTCCACTGAGAGTTTCAAGTGTGAAATATGCAATAAAAGGTATCTTC GAGAGAGTGCGTGGAAACAGCACCTTAACTGTTACCACCTTGAAGAAGGTGGAGTAAGTAAGAAGCAAAG AACTGGGAAAAAAATTCACATATGCCAGTACTGTGACAAACAGTTTGACCACTTTGGACACTTTAAAGAA CACCTTCGAAAACATACAGGTGAAAAACCTTTTGAATGTTCAAATTGTCACGAGCGGTTTGCTAGAAATA GCACTCTCAAATGTCACCTCACTGCATGCCAAACTGGAGTAGGAGCAAAAAAGGGCAGGAAGAAGCTTTA TGAATGCCAGGTCTGTAACAGTGTATTTAACAGCTGGGACCAGTTCAAAGATCACTTGGTAATACACACT GGAGATAAACCCAACCATTGTACTCTGTGTGACTTGTGGTTTATGCAAGGAAATGAATTACGGAGGCATC TTAGTGATGCTCACAATATTTCAGAGCGTATAGTAACTGAAGAAGTCCTTTCAGTAGAAACACATTTACA AACCGAGCCAGTGACATCAATGACTATTATAGAGCAAGTTGGGAAAGTGCATGTGTTGCCACTGCTTCAG GTCCAAGTGGATTCCGCACAAGTAACTGTGGAACAAGTTCATCCAGATCTGCTCCAGGACAGCCAAGTGC ATGATTCACAAATGACCGGGCTTCCAGAGCAGGTCCAAGTGAGTTATCTGGAAGTGGGCCGAATTCAGAC TGAAGAAGGCACTGAAGTACATGTTGAAGAGCTTCACGTTGAACGGGTAAATCAGATGCCAGTTGAAGTA CAGACTGAGCTTCTAGAAGCTGACTTGGATCACATGACCCCTGAAATCATGAGCCAAGAGGAGAGAGAGC CTAACCATGCAGATGCTGCCATGGAAGAACACGAAGATGCTGAGGGGTTAGAGACCAAACCAAGCGAGTA TTCCCAAGCTAGAAAAACAGAGAATGACAGGACATCTCTGCCGGTTTTAGAATGAAATAACAGTTATATT TTTAAATTTACATTTGTGGTAAACTGATGGGCAGTGTGACCTTAAGCTATCAAATAAGTGGACCAAAGTT AAACTATTTCTGTTGTGCTGAACTGTTTCTATGGAAACAGGCTGATGTCCTTCCAGTTAATGCCACAGAG TGGGAATATTCCTCATAATGAGTGGAAGCTTTGAGAAGTATATTTCTGTTAACTTAAATGAATTATATTC TTATTATATAGTTGGGTACGAATGCATCTATTTTCATTGTGGTAAGGGTCTCTGTATCCTCTTCCCCAGG TCATGTCTTTCCTTAAATTCTTTGCATATTATAAAATCATACTTAAAACCATTAGAATACGTACATTTGT ATTCTAATGCATGTTAAAAAGTTGAGTATGTAGGCAACACAAGACTGCATGAGGGAAGTGCATTGTTACG TGCAGTTAATAGTTGGGGGCTGGCTTTATTTTGAACAACATTCTTGTCTATCCAGTGGCTACAAATGTTG TCCCCACAACAGAAACGGTGGTGGCAGAATTTCTAGAATGTTAAAAAACAAAAACAAAACAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:5323559 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5323559 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002507 same experiment
	EMAGE:31978 same embryo
	EMAGE:31235 same embryo
	EMAGE:31239 same embryo
	EMAGE:32057 same embryo
	EMAGE:29810 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source