EMAGE mouse gene expression spatial database

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EMAGE:32057

Fcho1 ( MGI:1921265)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002537_01	euxassay_002537_02	euxassay_002537_03	euxassay_002537_04	euxassay_002537_05

euxassay_002537_06	euxassay_002537_07	euxassay_002537_08	euxassay_002537_09	euxassay_002537_10

euxassay_002537_11	euxassay_002537_12	euxassay_002537_13	euxassay_002537_14	euxassay_002537_15

euxassay_002537_16	euxassay_002537_17	euxassay_002537_18	euxassay_002537_19	euxassay_002537_20

euxassay_002537_21	euxassay_002537_22	euxassay_002537_23	euxassay_002537_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 18 weak expression: see section 11 17

not examined

regional

not examined expression: see section 01 02 22 23 24

neural retina

moderate

regional

moderate expression: see section 01 weak expression: see section 02 21 22 23 24

thymus primordium

weak

homogeneous

weak expression: see section 11 12 13 14 15

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 17 18 19 20 21 22

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 17 18 19

spinal cord

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 weak expression: see section 14 15 16 17

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 18 19 20 21 22 23 24 weak expression: see section 14 15 16 17

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5093
Entity Detected:	Fcho1, ( MGI:1921265)
Sequence:	sense strand is shown >T5093 CCACGCGTCCGCGCCGCGAGAGGCGATGGAGAAACTGAGGCACAGAGCTGAGTGAATTGGAGCCAGACAG ACGCAGGACAGAGGCCACCAGGATGTCCTATTTTGGGGAACATTTTTGGGGCGACAAAAACCATGGCTTT GAGGTCCTCTACCATTGTGTGAAGCAGGGTCCTGTGGCCACCAAGGAGCTGGCTGACTTCATTAGGGAAA GGGCCAACATCGAGGAGACATACTCGAAGGCCATGGCCAAACTCTCCAAGCTAGCCAGCAACGGGACCCC CATGGGGACCTTTGCCCCGCTCTGGGAGGTCTTCCGTGTGTCCTCGGACAAACTGGCACTGTGCCACCTG GAGCTTACTCGGAAGCTGCACGATCTCCTTAAGGATGTGCTGCGCTACGGCGAGGAGCAGCTCAAGACCC ACAAGAAGTGTAAGGAGGAAGTTCTAGGCACTGTGGATGCAGTGCAGATGTTGTCAGGCGTCGGGCAGCT CCTGCCAAAGTCTCGCGAGAATTACTTGAGCCGCTGTATGGACCTGGAGAGACTGCGTAGAGAGAACACC AGCCAGAAGGAGATGGACAAGGCAGAAACCAAAAGCAAGAAGGCTGCCGACAGCCTGCGGCGCTCTGTGG ATAAATACAACTCAGCCCGAGCCGACTTTGAGATCAAGATGCTTGATTCAGCCCTGCGTTTTCAGGCCAT GGAGGAGGCACATCTGCAGCACATGAAGGCCTTGCTGGGCTCCTATGCGCACTCTGTGGAGGACACCCAT GTGCAAATTGGGCAGGTGCATGAGGAATTTAAGCAGAATGTGGAAAATGTGACGGTGGACATGCTCCTCA GGAAATTTGCAGAGAGCAAGGGCACCGGGCGCGAGAAGCCTGGGCCTCTGGACTTCGATGCATACAGCTC AGCTGCCCTGCAGGAAGCAATGAAACGTCTCCGTGGAGCCAAGGCTTTCCGCCTTCCAGGATTGAGCCGT CGGGAGCCACGTGCATCTGTTGATTTCCTGGAGTCTGACTCAGGGGTGCCTCCAGAGGTAGATGACGAAG GCTTCACTGTGCGCCCCGATATATCTCAGAACAATGGGGCTGAGCCCCCACGCTTCTCCTCTAGTGACTC TGATTTTGACGATGAGGAGCCTAGAAAGTTCTATGTTCACATCAAGCCCGCCCCCACCCGCGCCGTGGCC TGCAGCTCTGAGGCTGCAGCTGCCCAGCTCAGGGCCACGGCTGGCAGCCTCATCCTCCCTCCAGGCCCAG GGGGCACCATGAAACGTCATTCATCACGGGACACTTCTGGGAAGCCACAGAGACCTCGTTCGGCCCCACG CACTGGCAGTTGCGCAGAGAAGCCCCTGGCCTCGGAGGAGCCACTGTCCAAGAGCCTCTTTGGGCCGCCG CTGGAGTCCGCCTTCGACCACGATGACTTCACAGGCTCCAGCAGCCTGGGCTTCACATCCAGCCCGTCTC CTTTCTCATCCTCATCTCCGGAGAATGTGGAGGACTCGGGCCTGGATTCTCCGTCACATGCTGCCCCTGG TCCGTCACCTGAGTCCTGGGTCCCCCGGCCAGGCACCCCGCAGAGCCCACCCACCTGCAGGGCACAGCAC CCTGAGCCCAGGGGCCTAATGCCCCGTGCACCCTCACCAGGTCCCTGGGGGCCTGAGGGAGGTGCAGATT CACTGACGCCGGCTGACCCCACCAGGGAGGGCCTAGCTGCCACACTGAGAAGACCACGGTCCAGGAAAGT GTCCTGCCCACTCACCAGGAGCAATGGGGACCTGTGCCGGTCGCTCAGCCCATCCCCACTGGGATCCTCT GCCCCCACCATCCCCCCCGATCGGCCTAGCTTCTCCACCCAGATGGGACATGGCATCTCCCGTGGCCCCA GCCCAGTGGTCCTGGGATCTCAGGACACCCTGCCCGTGGCCACAGCCTTCACTGAGTATGTCCATGCCTA TTTCCGTGGCCACAGCCCCAGTTGCCTGGCTCGAGTCACCGGGGAGTTAACCATGACCTTCCCTGCGGGC ATTGTGCGTGTGTTCAGTGGCACCCCACCACCACCTGTCCTCAGCTTCCGGCTGGTGAACACGGCCCCTG TAGAACACTTCCAGCCCAACGCTGACCTGATCTTCAGTGACCCCTCCCAGAGTGACCCGGAGACCAAAGA CTTCTGGCTGAATATGGCGGCGCTGACGGAGGCCCTACAGCACCAGGCTGAGCAGAACCCCACCGCCTCC TACTACAACCTGGTGCTATTGCGGTACCAGTTCTCCCGCCCTGGACCTGAGTCAGTGCCCCTGCAAATGA GCGCCCACTGGCAGTGCGGGCCCACACTCACGCGGGTCTCGGTGGAGTACAGCTACCGTGCGGGCGCCAC TGCTGTGTCCACGCCACTCACTAATGTCCAGATCCTCCTGCCCGTGGGGGAGCCGGTGACCAGTGTGCGG CTGCAGCCTGCGGCCACCTGGAACACAGAGGAAAAGAGGTTCACGTGGAAGCTTCCAGATGTGTGTGAGG CAGGGGGCTCAGGCCACCTGTCAGCCAGCTGGCAGCCGCAGTCGGGGCCCAGCACTCCCAGCCCCGTGGC CGCACAATTCACCAGTGAGGGCGCCACGCTGTCTGGCCTGGACCTGGAGCTGCTAGGTGGTGGCTACCGC ATGTCCCTGGTGAAGCGGAGATTCGCTACAGGGATGTACCTTGTCAGCTGCTGAGGTTCCTAGTGCTGGG GACCGCCTGCTCTCCTGCCTCTGCCGGCTCCCCCAGGCTGGACTCCGTGGACAGGAACACCCATTTCAGC CTGGCTCAGCCAACTCCTCCGTCCACAAGCAGGCCCTGGCCTTTAGTATTCTTTAAATAAACAGTTTTCT AACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5323527 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5323527 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002537 same experiment
	EMAGE:31978 same embryo
	EMAGE:30965 same embryo
	EMAGE:31235 same embryo
	EMAGE:31239 same embryo
	EMAGE:29810 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source