EMAGE mouse gene expression spatial database

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EMAGE:31978

Polr3k polymerase (RNA) III (DNA directed) polypeptide K ( MGI:1914255)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002548_01	euxassay_002548_02	euxassay_002548_03	euxassay_002548_04	euxassay_002548_05

euxassay_002548_06	euxassay_002548_07	euxassay_002548_08	euxassay_002548_09	euxassay_002548_10

euxassay_002548_11	euxassay_002548_12	euxassay_002548_13	euxassay_002548_14	euxassay_002548_15

euxassay_002548_16	euxassay_002548_17	euxassay_002548_18	euxassay_002548_19	euxassay_002548_20

euxassay_002548_21	euxassay_002548_22	euxassay_002548_23	euxassay_002548_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

weak

regional

weak expression: see section 09 10 16 17 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 20 21 weak expression: see section 03 04

not examined

regional

not examined expression: see section 01 02 21 22 23 24

neural retina

moderate

regional

moderate expression: see section 01 02 21 22 23 24

trigeminal v ganglion

moderate

regional

moderate expression: see section 20 21 weak expression: see section 03 04 05 06 07 08 16 17 18 19

cervical ganglion

weak

regional

weak expression: see section 09 18

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 08 17 18 19

cervico-thoracic ganglion

weak

regional

weak expression: see section 10 17

spinal cord

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07 18 19

thoracic ganglion

weak

regional

weak expression: see section 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3905
Entity Detected:	Polr3k, polymerase (RNA) III (DNA directed) polypeptide K ( MGI:1914255)
Sequence:	sense strand is shown >T3905 CGGCTGTTTAGGTCTCGACTTACATCAGAATCTTGCACAGCATTGCATATTTTGGTCAGTGTGTCCTAGA GGGAGCACTGGGATTTTGCAATGCTGTGCAAGGGAAGCTGGCAGTATGCTGTTGTTCTGCCCGGGCTGCG GGAACGGACTCATTGTGGAGGAGGGGCAGCGTTGCCATCGCTTCGCCTGCAACACTTGCCCCTACGTCCA TAATATCACCCGTAAGGTAACAAACCGAAAGTATCCAAAGCTAAAAGAAGTGGACGACGTGCTTGGTGGA GCAGCTGCATGGGAGAATGTGGACTCTACTGCAGAGCCATGTCCAAAATGCGAACATCCCCGTGCCTACT TCATGCAGCTTCAGACGCGTTCTGCAGACGAGCCAATGACCACCTTCTATAAGTGCTGCAATGCTCAGTG TGGACATCGCTGGAGGGATTAAGGGCAGGATTTGCCCAGCTGCAAGCATGAATTTTGTGTGTACTGAAGG TCTTTGCTATATGATAGTAAGCTGACTTTTCTGTGGTGAAGGATCAGGGTGAAATATAACTCATCTGCAA GTTAACAGATAAAGTCCTTGATGTATAGGGGCCGTCAGGTCCTTGGGAGATACACATAAGGATGCTCACT TGGGTTCAGGCCCTGTTCCTTACCATTCAAACTAGGAACTTGGTTTATAGTCAGTAAAAATAAAAATTAT TATTATTATTTAGTTTTTCAAGACAGGTTTTCTCTGTGTAGTCCTTGCTGTCCTTGTTATGTAGACAAGT CAACCTTGAACTCAGTGATCCACCTTCCTCTGCCTCCAGAGTGCTGGGGTTAAAAGTGTGTGCCATGACC ACCAATGATAACCTTGAACCTTCTAATCTTTTTGCCTCTTGTTGGAAAAGTGTTAGGGTTATAGGCATGT GAACTATTTTCATTTTATGCAGTTCTGGGGATTAAACTCTAAGCCTCATGCATGCTAGATAAGCATTCTA CCAACTGAGCTATATCTCTAGCCCTTTTTTTCTTTCTAGAGAAATTATGAAAAATACTTATAAAGATACT GAAAAATCACTTTAAAAGTCTGGAATTACTCGATTCATCTTGTATGATTAATAACTAAACAAAACTATTC TGTGAGTTGGACTTTCTGGATGATTTTGATAGGAATTTTAAAAAGTAACCAAAACCATTTTCTATTACCT GATTTGGAGAAAGGTCTACAGTAGAGCTTGTGCCCCATTTGAACCCCATCCAAAAATCTATTTTAACCTC AATAAATTTAGAAAAGTTACATATTAAGGCTATGTGAAGAATGAGCTTATATATGGACCAACTTCAGATG AGAGTGTCTGAGAGTATCTCAGAGAGCTCACAAGCACGAATTCCTCTAACAAACAAGAGTAACAGAAAAG TTGCATATTTGTTGAAACCACTAATGGTATTGCTCTGTAAGGTGGACATATTTGTATGTGGGAAGTCCTG GTCTGTTCTGTGTAACTGGACAGTTCTACACATGATAAGAAATGTAGCTAGTAAGGAAATCTAAAATAAC AGATTCCTCCCATGAGTATCTGATGTTTTCAAAATCCTTAAATAGTGATATAGATGAATAATTCATAAAC CAAAGCTGACTTTGAAATTTTTGATCTCTGGTTGCTTTCAAGCCTTTGGAACTTTGTTGCTTATCTCCTT TAAATAACTCCGTTACAAAGTAGAGCCACGAAGTCTTCTTTGAATGAGGTGTATTGAGTATTAAAGCACT TGTATATGCCAAGCTCTGGCCTCAGTTCTTGTACTGGATGGCAGAGGTGTGTGGAAGAATGAGGATGTTT ACTGTTGGTGAGGGTTAGGACATTCTCACCTTGCTGGTGGGAGTAGAAGATGGTACAGTATAGGAAGGGA AGATAAAGCCCCATGCTTCTGCCTGCGTCAATCTCAGTTCGTTCAGTAAACTTTTCTCAGCAAAGCTGGT ATTTTGATTATGTAGCAGGATTTTCCCTGTCCAATTAATTTAATTATTAATACAATAAGAAGCCTGTGAT TGGACAGAGTTGTAGAGATAGGGAGCCACAGAGGAGAAGAGGAGGAAGAAAATGGAGGACAAACAGGACA ATTCAGATTCTGCGTGGCTTTAAATAGCCACAGGTAGCTGTAGTATAGGGATAGAATAATTGGGATAACT TGTCTAATCTAGGTGGGCAGCTTGTATCATCGATTGACTCTGAAATTATTGTGTGGGTATCTTGTGAATT GAGAATTTATTGATATATAAATCTGACTAATTATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5253879 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5253879 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002548 same experiment
	EMAGE:30965 same embryo
	EMAGE:31235 same embryo
	EMAGE:31239 same embryo
	EMAGE:32057 same embryo
	EMAGE:29810 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source