EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31235

Thtpa ( MGI:2446078)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002420_01	euxassay_002420_02	euxassay_002420_03	euxassay_002420_04	euxassay_002420_05

euxassay_002420_06	euxassay_002420_07	euxassay_002420_08	euxassay_002420_09	euxassay_002420_10

euxassay_002420_11	euxassay_002420_12	euxassay_002420_13	euxassay_002420_14	euxassay_002420_15

euxassay_002420_16	euxassay_002420_17	euxassay_002420_18	euxassay_002420_19	euxassay_002420_20

euxassay_002420_21	euxassay_002420_22	euxassay_002420_23	euxassay_002420_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon marginal layer

strong

regional

strong expression: see section 03 04 05 06 moderate expression: see section 01 02 07 08 09 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5088
Entity Detected:	Thtpa, ( MGI:2446078)
Sequence:	sense strand is shown >T5088 CCACGCGTCCGCGGACGCGTGGGCGGACGCGTGGGGGAAAAATGCAGGATGAACAGCGGGCAAGAGGAAG CTCTCTCTTCTCTTGCCCACCCGGTTGGGTCGCACGTAACCTAACAGACCTCTCAGGCCTAGCTTGTCAT CGGCACCGCCACACCGGTCTAATATGGCCGCTAGTGCTGGGACCGGTCTCGCGCGTAAAGAGCCTGGACT AAGGTCCGGAGCCGGAGACCGCTCCCGGCATTCCTCGCGGGTGTAGCGTGGGCACCCTTCCCCCACTTCC GGGTCCCGGGAGAAGGAGACCCTGGGGTCTTGAAGAGTGTCCCGAGGTACCGCTGTTTGGTGCTAGGCGA AGAATGGCAGATCATCCTGGGCAGTGAATCACTGGCCTTGGGTAGCAAGGTGTAGCGAGAAGATAGGTCA TCAGCATTACGGCCTGTTTGCTAGGGAGCCTTTTGGGTTATCAGTAGTAGGATTTAACTGGAGACTTGTC ACCATTGTTGCAGCCCGCAGAGACCTCCAAGTAGAGCCCTGTGAACCTGGAGCAGAGCTCTGGGACAGTG TATATGCATCTTAAATTGATCCAGGACTAGTCCGTCTTTGCTGACTAGTCCTCACCTTGAGCACTCAGTG AATACCTGGCAAACAGGGTTGCAGGTTAATATGGCCCAGGGCTTGATTGAAGTGGAGCGAAAGTTCGCTC CAGGGCCTGACACGGAGGAAAGGCTGCAGGAGTTGGGGGCCACCCTGGAGCACCGGGTCACCTTCCGAGA CACATACTATGATACCTCTGAGTTAAGCCTTATGCTGTCTGACCACTGGCTGCGACAACGAGAAGGTAGT GGATGGGAGCTCAAATGTCCTGGAGTAACAGGTGTCTCAGGACCTCACAATGAGTATGTGGAAGTCACAT CGGAAGCTGCGATTGTGGCCCAGCTCTTTGAACTGCTCGGGTCTGGGGAGCAGAAGCCTGCAGGTGTGGC TGCTGTCCTGGGCTCGCTGAAGCTGCAGGAGGTGGCTAGTTTTATTACTACGCGAAGCTCCTGGAAGCTG GCCTTATCTGGAGCCCATGGACAGGAGCCTCAGCTCACAATAGACTTGGATTCAGCGGACTTCGGCTATG CTGTGGGTGAGGTAGAGGCCATGGTGCACGAGAAGGCTGAAGTCCCGGCTGCCCTAGAGAAGATCATCAC CGTCAGTAGCATGCTTGGAGTGCCAGCACAGGAGGAGGCACCAGCCAAGCTCATGGTATACCTACAGCGC TTCAGGCCTCTGGACTATCAGCGCCTGCTAGAAGCAGCCAGTTCTGGTGAGGCCACAGGGGACTCAGCAT CCTGACAACAGCGTTCACTTTCCAAAAGGGAAGGTAACTCTGAAGCCGCCATTCACATCCGGACCCACGG TGCTCCTTTCCCTTACAACGGAGCCACTTTCCCCTCCAGCTCTGCCTGGTCTTTCGCCCACTCTGAGCTA TCCTTCCTGGAGCCCTCCCTGGCTGCTTCCCTCATCCGTCAGATGCAATCTGTGGGCCGCCCCTCTCCCC TGGCCGCTAAGCAGCCTCCATTGATTTCCGCTCGTGTTTATAGGATTTCCACTTAGCCGTGATCAGTAGT TAAGCACAGGAAAATCCCTTGCCACCCCCCCCTCCCTTGGTCGATGCCATTGATTCTGCCAGAGGCTCTG AAGCCGCCTTACAGCTGAGTTAGAGATGAGGGGAAACAGCGGGGATTTCCCTGCCCTGGGGTGTAGGGAA GAGCAAGAAGGTGGGGAAAAGGTTAGGAACTCCTTAAGGGATCTAGAAATTCAGGGCCTGCTGCTGTGCC GGGAGGGTAGAGTGCTCAGTGTGGCAGTTTAGAGAGAACATTTGCTCTCCAACCCAGAGAGGGGTAGAAA GAAAATGGTCATTGTTGAGACCGGTTCCTAGGGTTTCAGGAAGTCATACTTCCTGTGGAGAAGGCACCAC CCTTGCTCTTAACACATTTTTTTTTCTCTCAACTAGGTCAAAACAGGCCTCTCTTCCCTTCTCACGCTTC ATAGACTTTATTTGTTGGGGAGGAGGCAGGCTCTCTATATTGCCTTGACAAATATAGGGTCTGAGTGCCA CCTGCTGGTGGCCCTCAGGGTTGTGTCCCAAGCTGAGGGTGCAGAAAGATTTGAGAGGAGCCGAGGCAGT TATGAGGCAGGCAAGCTTAGGGAACAGGTTAGCAGCCTGGAACTCCACTTGGTTTGAGCTATGCACACCT CTCATCTCACTGCCACTTGGCATCTTAGGACCCTCAGCTGCACGCACCATGCACACACATTCTTCTAACA GTGCAGCCAGGACCCCGCCTGTATCGAGTAGCACTTAGAGCCCACTTCAGACAGTAAATCTCTCTTTCCC CAGCAGCCATCAAAACTCCCCTTCTGTGGACAGTTTTATTCAAACACTTTAGGACATACCCCAAAGTAGG GAGCTGATTAGGCCTAAACGGGTTTGGGGTAACCTGTGATCTTTGGGTGACCTTTTGAGGAGAACTCCCT ATGGACCGTCCTATGCCTGTGCAGAATGAGTTAGCTTATGATTAAAGTCAGCTGCATCATGGGAAGGGTT ACGCACAGAGAAGAAAGGCTGACAGAATCCAAGCCTGTCAATGAAAACAGAGAATCACTCAAATTATGCC ACTTCACAGCTAACACCTTCGTTCCCTGAGACTTTATTAAAGGAACACCGGGTGACTGCCCTGGGCTCTT AAGACTGTGGTCATCACCCGTTTACAGAGCACTTCTAATTTGTACGCTCAGTTTGCCTTTAAATAAAGTT TACTTCGCCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5322971 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5322971 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002420 same experiment
	EMAGE:31978 same embryo
	EMAGE:30965 same embryo
	EMAGE:31239 same embryo
	EMAGE:32057 same embryo
	EMAGE:29810 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source