EMAGE mouse gene expression spatial database

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EMAGE:31729

H47 ( MGI:95994)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_009232_01	euxassay_009232_02	euxassay_009232_03	euxassay_009232_04	euxassay_009232_05

euxassay_009232_06	euxassay_009232_07	euxassay_009232_08	euxassay_009232_09	euxassay_009232_10

euxassay_009232_11	euxassay_009232_12	euxassay_009232_13	euxassay_009232_14	euxassay_009232_15

euxassay_009232_16	euxassay_009232_17	euxassay_009232_18	euxassay_009232_19	euxassay_009232_20

euxassay_009232_21	euxassay_009232_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

maxilla

moderate

regional

moderate expression: see section 03 05 06 07 08 09 15 16 17 18 19

mandible

strong

regional

strong expression: see section 01 02 moderate expression: see section 03 05 06 07 08 09 15 16 17 18 19 20

palatal shelf

moderate

regional

moderate expression: see section 15 16 17

clavicle

moderate

regional

moderate expression: see section 07 08 14 weak expression: see section 15

rib

moderate

regional

moderate expression: see section 19 weak expression: see section 01 02 03 14 15 16 17 18

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 moderate expression: see section 20 21

viscerocranium

moderate

regional

moderate expression: see section 07 08 09 15 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2547
Entity Detected:	H47, ( MGI:95994)
Sequence:	sense strand is shown >T2547 AATTCGGNCGAGGGGAAGGCCTCAGGAAGAAGATGGTCCTGGACCTTCTACTTCATCTGTCATCCCCAAA GGAAAATCTGACAAAAAGCCTTTGCGAGGAGGTGGTTATAACCCTCTGACGGGTGAAGGGGGTGGAACCT GCTCCTGGAGACCTGGACGCAGGGGCCCATCATCTGGCGGCTGAAACTAAGACTCTTGTTAGTGTCGCTC TGACATTAGCAAGGTGAACCTTTAACCCTCAACTCAATTGCCTTACGCACACTTTCACAGTGACTGGCCA AGGAGAGGTGGGGCTTTTCTCTGTTCTAAACTACTTGTACTTTAAGGGCTTTGGTCAGCATGAGATATAG ACATTGCCATTAGGCCACACTCTAGACAAGACAGCCATGGCTTTTATGGCTGCTGGCTAGTTGGTAGGTT GAAGGCTTCTTGCTGTTTAGCAGACTTCATAAAGAAGGCCCAGTGATGATACTTTGGGGTAGAAGTCCTT GCTGGCAGGATGGTCTCTGTGACGGGATGCG
Notes:	The probe template was PCR amplified from IMAGE:1382283 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1382283 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009232 same experiment
	EMAGE:29739 same embryo
	EMAGE:30960 same embryo
	EMAGE:30963 same embryo
	EMAGE:31446 same embryo
	EMAGE:31731 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source