EMAGE mouse gene expression spatial database

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EMAGE:7111

Lyrm4 LYR motif containing 4 ( MGI:2683538)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002591. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002591_01	euxassay_002591_02	euxassay_002591_03	euxassay_002591_04

euxassay_002591_05	euxassay_002591_06	euxassay_002591_07	euxassay_002591_08	euxassay_002591_09

euxassay_002591_10	euxassay_002591_11	euxassay_002591_12	euxassay_002591_13	euxassay_002591_14

euxassay_002591_15	euxassay_002591_16	euxassay_002591_17	euxassay_002591_18	euxassay_002591_19

euxassay_002591_20	euxassay_002591_21	euxassay_002591_22	euxassay_002591_23	euxassay_002591_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7111_wholemount_strong.wlz

7111_wholemount_moderate.wlz

7111_wholemount_weak.wlz

7111_wholemount_possible.wlz

7111_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7111_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5217
Entity Detected:	Lyrm4, LYR motif containing 4 ( MGI:2683538)
Sequence:	sense strand is shown >T5217 GGGTTCAGTTAAACTTGGGGCTTCTACGAAGAAGGACCTGTTTGGTTTTGTTGTTAGGGTGTTTTTTGGT TTGTTTGTTTTTGTTTATTTTCAACATGGCAGCCTCCAGCCGCGCACAAGTTTTAGATCTGTACCGGGCG ATGATGAGAGAGAGCAAGCATTTCAGCGCCTACAATTACAGAATGTATGCTGTCAGGAGAATAAGAGATG CCTTCCGAGAAAATAAGAATGTAAAAGACCCTGTAGAAATTCAAGCCCTGGTGAATAAAGCCAAAAGAGA CCTTGAAATAATCCGCAGACAGGTCCATATTGGCCAACTATACTCAACTGACAAGCTTATCATTGAGAAT CAAGAGAAGCCCAGGACATAGGAAGCCAGCACAAGGAAACAGCCTCCACAGCAGAACTGACCACCTCCAG CATCCATTCTATATGGTGGAACCTCCCAGTACTGGGTGAAATAGCCACTGCTCTGCCTGCCTTGTAGGCG CTGATAAACTGAGTCCCATTTGCATTCTGTCACAGCTGTTATGTGAAGAAATGGCTGTGTATCCTCATTT CGGGTGTAAGAGTGAAGAGCTGGAGGTCACTCAGAATAATGCTATATGTTATTGTGTCTCCTCGCCATCC TACCCCATCTTAAAGATCACCCACATTTGTATAGAGCTTTTGCAGACTCTGTGTCTTGGTCCTTAACAGT AAACATAATTATCTCTCTATCCTTTTGTACCAAATAAACCCATTACAATGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5355517 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5355517 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7114 same embryo
	EMAGE:7109 same embryo
	EMAGE:7110 same embryo
	EMAGE:7113 same embryo
	EMAGE:7112 same embryo
	EurExpress:euxassay_002591 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source