EMAGE mouse gene expression spatial database

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EMAGE:7112

Nfatc1 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 ( MGI:102469)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002607. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002607_01	euxassay_002607_02	euxassay_002607_03	euxassay_002607_04

euxassay_002607_05	euxassay_002607_06	euxassay_002607_07	euxassay_002607_08	euxassay_002607_09

euxassay_002607_10	euxassay_002607_11	euxassay_002607_12	euxassay_002607_13	euxassay_002607_14

euxassay_002607_15	euxassay_002607_16	euxassay_002607_17	euxassay_002607_18	euxassay_002607_19

euxassay_002607_20	euxassay_002607_21	euxassay_002607_22	euxassay_002607_23	euxassay_002607_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7112_wholemount_strong.wlz

7112_wholemount_moderate.wlz

7112_wholemount_weak.wlz

7112_wholemount_possible.wlz

7112_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7112_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5205
Entity Detected:	Nfatc1, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 ( MGI:102469)
Sequence:	sense strand is shown >T5205 CGAGAGGCTCCGAACTGGGCGGCAGAGTCGCCGCGCGGATCGGAGCGGCGGGGCACGAGCTCCCCGGCGC CGCACACACCTCCGCCCCTGCGGCGAGAGTCCCCGGCCGCAGGCAGGCCAGCCACGCGCGCACACACCCC GCATGTCACCTCCCCGCGGCGCACAGCGCTGAGCCCGGCCCGGCGCGTGTCGGCAAAGGAGAGGTCGGAC TCGGGAGCCGGCCCGGAGCTGCTCCCGGCGCCAGCTCCTGCTCCTCCTCCTGCTGCTCGCCGCTGCCGCC ACCGCCGCGTGGATGCCAAGTACCAGCTTTCCAGTCCCTTCCAAGTTTCCACTCGGCCCTCCGGCCGCAG TCTGCGGGAGCGGAGAAACTTTGCGGCCCGCGCCGCCCTCCGGCGGCACCATGAAGGCGGCCGAGGAAGA ACACTACAGTTATGTGTCCCCTAGTGTCACCTCGACCCTGCCCCTTCCCACAGCACACTCTGCCTTGCCA GCAGCATGCCACGACCTCCAGACGTCCACCCCGGGTATCTCAGCTGTTCCTTCAGCCAATCATCCCCCCA GTTACGGAGGGGCTGTGGACAGCGGGCCTTCGGGATACTTCCTGTCCTCTGGCAACACCAGACCCAACGG GGCCCCGACTCTGGAGAGTCCGAGAATCGAGATCACCTCCTACCTGGGCCTACACCATGGCAGCGGCCAG TTTTTCCACGACGTGGAGGTGGAAGACGTACTTCCTAGCTGCAAGCGCTCACCGTCTACAGCAACCCTGC ACCTGCCCAGCCTGGAAGCCTACAGAGACCCCTCCTGCCTGAGCCCAGCCAGCAGTCTCTCCTCCAGAAG CTGTAACTCTGAGGCCTCCTCCTACGAGTCCAACTACTCCTACCCATACGCGTCCCCCCAGACCTCTCCG TGGCAGTCACCCTGCGTGTCTCCCAAGACCACGGACCCGGAGGAGGGTTTTCCCCGAAGCCTGGGTGCCT GCCACCTGCTAGGATCGCCCAGGCACTCCCCATCCACCTCTCCTCGGGCAAGCATCACGGAGGAGAGCTG GCTCGGTGCCCGCGGCTCCCGGCCCACGTCCCCCTGCAACAAGCGCAAGTACAGTCTCAATGGCCGGCAG CCCTCCTGCTCACCCCACCACTCACCCACACCATCCCCCCATGGCTCCCCTCGGGTCAGTGTGACCGAAG ATACCTGGCTCGGTAACACCACCCAGTATACCAGCTCTGCCATTGTGGCAGCCATCAACGCCCTGACCAC CGATAGCACTCTGGACCTGGGTGATGGGGTCCCTATCAAGTCTCGAAAGACAGCACTGGAGCATGCGCCC TCTGTGGCCCTCAAAGTAGAGCCAGCTGGGGAAGACCTGGGCACCACTCCACCCACTTCTGACTTCCCAC CCGAGGAGTACACCTTCCAGCACCTTCGGAAGGGTGCCTTTTGCGAGCAGTATCTGTCGGTGCCACAGGC CTCGTATCAGTGGGCGAAGCCCAAGTCTCTTTCCCCGACATCATATATGAGCCCATCCTTGCCTGCCCTT GACTGGCAGCTCCCGTCACATTCTGGTCCATACGAGCTTCGGATCGAGGTGCAGCCCAAGTCTCACCACA GGGCTCACTATGAGACGGAAGGCAGCCGGGGGGCTGTGAAGGCTTCAGCTGGAGGACACCCCATTGTGCA GCTACACGGTTACTTGGAGAATGAACCTCTCACGCTACAGCTGTTCATTGGGACGGCTGACGACCGCCTG CTGAGGCCCCACGCCTTCTACCAGGTCCACCGGATCACGGGGAAGACTGTCTCCACCACCAGCCACGAGA TCATCCTGTCCAACACCAAAGTCCTGGAGATCCCGTTGCTTCCAGAAAATAACATGCGAGCCATCATCGA CTGTGCTGGGATCCTGAAGCTCAGAAACTCTGATATTGAGCTGAGGAAAGGGGAGACAGACATCGGGAGG AAGAACACCAGGGTGAGGCTGGTCTTCCGAGTTCACATCCCACAGCCCAATGGCCGGACGCTGTCTCTCC AGGTGGCCTCGAACCCTATCGAGTGTTCCCAGCGGTCAGCCCAGGAGCTGCCCCTCGTGGAGAAGCAGAG CACAGACAGCTACCCAGTCATCGGCGGGAAGAAGATGGTGCTGTCTGGCCATAACTTTCTGCAAGACTCC AAAGTCATTTTCGTGGAGAAGGCTCCAGATGGCCACCACGTCTGGGAGATGGAAGCAAAGACTGACCGGG ACCTGTGCAAGCCAAATTCCCTGGTGGTTGAGATACCACCTTTCCGCAACCAGAGGATAACCAGCCCCGT CCAAGTCAGTTTCTATGTCTGCAACGGGAAACGGAAGAGAAGCCAGTACCAGCGTTTCACGTACCTTCCT GCCAATGTTCCAATTATAAAGACAGAACCCACGGACGACTTTGAGCCAGCTCTGACCTGTGGACCAATGA GCCAGGGGATTAGTCCTCTGCCGAGGCCTTACTACAGCCAACAGCTCACCATGCCTCCCGACCCCGGCTC CTGCCTCGTGGCTGGCTTCGCCCCCTGCTCCCAGAGGAACACGCTGATGCCCACGCCTCCCAACGCAAGC CCGAAGCTCCACGACCTTTCCTCTCCTGCCTACACCAAGGGCCTCACCAACCCGGGCCACAGTGGTCACC TTGGACTTCAGCCACCCGCTTCGGAGGCCCCCACCATGCAGGAAGTGCCGAGACCCATGGCCATCCAACC CAACTCGCCTGAGCAGCCCCCATCCGCCAGGCTACAGCCGCAGTAAATGAGATCATACGTAACGACCTCT CCAGCACGATCCCCCACTCCTAATTCAGCACATCGGAGCTGGCAGAAGCTGTGAGGAGTTGGCTCAGTGC AGACAACCGCGCTTCAGCAAGCGAGACTTTGATGAGATAAACTGGACCCACATCTGGTACCACTCAGAAA TCCCAACTTACCGAACGAACGGAACGCTAGGAGCTTATGTCTGAAGAAGTAGCTCTCCAACAGGGACGGA GGAGCAGGAGAGGGCCCACTGCATCTCCTGAAAGAAACACCATCTCAAGGAACGAGAAGGGCTGGTCAGT GAGGCCAGGGGCAGACGCTGGTCCACCGTGTGGCAGACTGTCCCCAAGCCTGACCCTCCTGCCCACTGGA TCAAAACACTGGAAGTGCCTTATTTAGTCTGACCACACCAGGGCATATGGAAGTGAGCACTGAATTTTCT ACCATGAGAGTATTTGTGGGAGCCAAAGCTATTAAAGAACACTTCCTTAGGAGGTTTTATCTTTTGTATT AATTGATAGGATTCTGAAGAGCCTCCTGCGTATCTCTAGCTTTTCTGGCTGCCCTACACAATTTGCCTGC CTTATCCAGTGCATTTTAGAGGTCTTCCAGCCTGTCTTCTTGGCTCTCTCATAGTCAGCCCCCTCCTCTG TGTGACTGAAACCACAGGTCTGTTAATAATAGCATGCTAGGATTCTTGTTCTCAATCCAGCTTCGAACAT AGTGAAGGAAGTCGAACAGCACAAGGCAGGGTCCTGGACAGAACAAAACCTGCCTGCTACAGGACAGAGC TTGTGTTTGCATGCATGTACATATAGGCATATATTTATGTATAGATATCCAAGGACAGAGGTGGCAATGA GCAGTCTGTGCCCACCAGGGGCCTTCCTTCCATGTTAGCAATAACCAGTATCCACCTTGAGAGTGCACCT CAGAGTCCAGAACCGGCTTCCCACCATCATGGTCTTTCCTTCCATCAGGGTCTACCTCTGGACAGGCAGG TGGCCTTTTCTTGCCTCACGCAGTGACTGAGCACTCCAGAGCTGTGCTCAAATGCCCATCAGCTACCAAA TGGCAAAATCTGAAAGTGGTTGTAAATAACCATTACAGAATGAGTGTAGTATATTTGTTCAATTATAAGA TTATTCTTTCACAGAAGCCTTATAGCTCTCTGCTTCATCTAAGAAAACAATTACCAAAAAAAACAACGTT TCTAACTGCAATCTGTGAACTGTGCGTTTTCAGATTGGTTACTGGTAACAGATAAGCTGGTGTCTGCTCT GTGTAATTAGCTGCTTACTTCAGTTACTAGCAGTGACCTATTATTTCTTATAACCAAAAAAAGCATGGTT TAATTAAAACATGTTTAATGATCGTGCCTTAGGAGTTAATGCCCCCTTATGGAACACGCCTGAATTGCAC CTGTGGCTGGAAGTTTTAAGTTACTCCCAGACAGATGGACTCATGACAGGAAAAGCTCTCTCACAGGAAG ATGCATCTTTAAAATTTTTGTCAGTCTGTATGATGGTGGCTTACCTTTCCCAACGCACAGAAAGAAACAA CTGTCTGAAAGCATACTGAATGATTTCGCACGACTGTGAAGAGCTGGCGCGAACTGCCTTGTACACACAT AGCTCCTGGCCGCCTGCAGGCTGCCTCCCGCCTGCCTCTCGTCTGTACCCCATGTTTATTAGCATCATGG AGTTGCATGAACCATTCTTAGTAGACTGTCATCTGAAAGCAAGCGTTTGATATTTGTGTCAGCTATCTTT GTAGTTAGGAGATGAATCCAATAAAGCAGTATTTTTTTTCTTTTAAAAAAAAAACAAAAAAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5354603 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5354603 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7114 same embryo
	EMAGE:7109 same embryo
	EMAGE:7111 same embryo
	EMAGE:7110 same embryo
	EMAGE:7113 same embryo
	EurExpress:euxassay_002607 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source