EMAGE mouse gene expression spatial database

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EMAGE:7113

Gtf2f1 general transcription factor IIF, polypeptide 1 ( MGI:1923848)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002597. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002597_01	euxassay_002597_02	euxassay_002597_03	euxassay_002597_04

euxassay_002597_05	euxassay_002597_06	euxassay_002597_07	euxassay_002597_08	euxassay_002597_09

euxassay_002597_10	euxassay_002597_11	euxassay_002597_12	euxassay_002597_13	euxassay_002597_14

euxassay_002597_15	euxassay_002597_16	euxassay_002597_17	euxassay_002597_18	euxassay_002597_19

euxassay_002597_20	euxassay_002597_21	euxassay_002597_22	euxassay_002597_23	euxassay_002597_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7113_wholemount_strong.wlz

7113_wholemount_moderate.wlz

7113_wholemount_weak.wlz

7113_wholemount_possible.wlz

7113_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7113_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5214
Entity Detected:	Gtf2f1, general transcription factor IIF, polypeptide 1 ( MGI:1923848)
Sequence:	sense strand is shown >T5214 CTCTTTTTCTCGTCTTCCAGCATTCTCCGAGACTTAAGGATTTGGAAATCCAAAGCACAGCATCACACTA ACGTGACAACCAGGAAAGGTGCCTTCCCTTTCCTGGAGCCGAGGGACACAACGAAGTTCATCCCGTCAGG CCGACCGGGAGCAGCTTGAGCAACGGCCATGGCGGCTCTAGGCTCCAGCAGCCAGAATGTCACCGAGTAC GTCGTCCGAGTCCCCAAGAACACAGCCAAAAGATACAACATAATGGCTTTTAATGCAGCCGATAAAGTCA ACTTTGCTACCTGGAACCAGGCACGGCTGGAGCGAGATCTGAGCAACAAGAAGATTTACCAGGAAGAAGA GATGCCAGAGTCCGGTGCAGGCAGTGAATTCAACCGCAAGCTTAGGGAGGAGGCGCGCCGGAAGAAGTAT GGCATTGTCCTAAAGGAGTTTCGGCCTGAGGATCAGCCTTGGCTACTCCGTGTCAATGGCAAATCAGGAC GGAAGTTCAAAGGCATTAAGAAAGGCGGGGTAACAGAGAACACAGCCTACTACATCTTCACACAGTGTGC TGATGGTGCCTTTGAGGCTTTCCCTGTACAGAACTGGTACAATTTCACACCACTGGCCCGACACCGCACA CTCACTGCTGAGGAAGCCGAGGAGGAATGGGAGAGGAGGAACAAGGTCCTAAACCACTTCAGCATCATGC AGCAGCGGCGGCTCAAGGACCAGGACCAGGATGAAGATGAGGAGGAGAAGGAGAAGCGCAGTCGCAAGAA GCCTAGTGAGCTGCGCATTCATGACCTGGAGGATGACCTGGAAATGTCCTCCGATGCCAGCGATGCCAGT GGTGAAGAGGGCAGCAGAACCTCCAAGGCTAAGAAAAAGGCCCCTGTGACCAAGGCAGGCAGGAAGAAGA AAAAGAAGAAGGGCTCAGATGATGAGGCTTTTGAGGACAGCGATGATGGGGACTTCGAGGGCCAGGAGGT AGACTACATGTCTGATGGCTCCAGCAGCTCTCCAGATGAGACAGAAGGCAAGCCCAAAGTGCCCCAGCAG GAGGATGGCCCAAAGGGGGTGGATGAGCAGAGCGAGAGCAGTGAGGAGAGTGAGGAGGAGAAGCCCCCCG AGGAGGACAAGGAGGAGGAAGAAGAGAAGAAGGCCCCTACCCCACAAGAGAAGAAGCGCAGGAAAGACAG CAGTGATGACTCAGACAGCTCAGAAGAGAGCGACATTGACAGTGAGACCTCCTCTGCACTCTTCATGGCG AAGAAGAAGACACCCCCAAAGAGGGAGCGGAAGCCATCGGGGGGGAGTTCAAAGGGCACCAGTCGGCCAG GGACTCCCAGTGCAGAAGCAGCAAGCACCTCTTCTACCCTGCGGGCTGCAGCCAGCAAGCTGGAGCAGGG GAAGCGGACGAGTGAGACTCCAGCAGCCAAGCGTCTTCGGATGGACACGGGTCCCCAGAGCCTGTCTGGG AAGTCCACACCCAGCAGCGGTGATGTCCAGGTGACAGAGGACGCTGTGCGCCGCTACCTGACCCGCAAGC CCATGACCACAAAGGACCTGCTGAAGAAGTTCCAGACCAAGAAGACAGGGCTGAGCAGCGAGCAGACAGT AAATGTGCTGGCGCAGATCCTCAAGCGCCTCAACCCTGAGCGCAAGATGATTGGTGATAAGATGCACTTC TCCCTCAAAGAGTGATGCCAATAAAGAGAGCAGGCCCAGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5355475 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5355475 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7114 same embryo
	EMAGE:7109 same embryo
	EMAGE:7111 same embryo
	EMAGE:7110 same embryo
	EMAGE:7112 same embryo
	EurExpress:euxassay_002597 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source