EMAGE mouse gene expression spatial database

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EMAGE:7232

Mvp major vault protein ( MGI:1925638)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004411. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004411_01	euxassay_004411_02	euxassay_004411_03	euxassay_004411_04

euxassay_004411_05	euxassay_004411_06	euxassay_004411_07	euxassay_004411_08	euxassay_004411_09

euxassay_004411_10	euxassay_004411_11	euxassay_004411_12	euxassay_004411_14	euxassay_004411_15

euxassay_004411_16	euxassay_004411_17	euxassay_004411_18	euxassay_004411_19	euxassay_004411_20

euxassay_004411_21	euxassay_004411_22	euxassay_004411_23	euxassay_004411_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7232_wholemount_strong.wlz

7232_wholemount_moderate.wlz

7232_wholemount_weak.wlz

7232_wholemount_possible.wlz

7232_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7232_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

single cell

moderate expression: see section 01 02 03 04 05 18 19 20 21 22 23 24

humerus

moderate

single cell

moderate expression: see section 23 24

femur

moderate

single cell

moderate expression: see section 02 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 16 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 16 17 18 19 21 weak expression: see section 08 20

rectum

moderate

regional

moderate expression: see section 12

midgut

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21

mandible

moderate

spotted

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 18 19 20 21 22

maxilla

moderate

spotted

moderate expression: see section 06 07 08 09 10 11 12 13 16 17 18 19 20 21 22 23

pectoral girdle and thoracic body wall skeleton

moderate

single cell

moderate expression: see section 01 02 03 04

pelvic girdle skeleton

moderate

single cell

moderate expression: see section 07

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6420
Entity Detected:	Mvp, major vault protein ( MGI:1925638)
Sequence:	sense strand is shown >T6420 TCCTGACCTGTGGCCTGCAGCAGCCAGCTACCCTGCTAAATGTTAGGCCGGGGCTTGGGATTCACCATGG CAACTGAAGAGGCCATCATCCGCATCCCCCCATACCACTACATCCATGTGCTGGACCAGAACAGTAATGT GTCCCGTGTAGAGGTTGGACCAAAGACCTACATCCGGCAGGACAATGAGAGGGTACTGTTTGCCCCAGTT CGCATGGTGACGGTCCCACCACGCCACTACTGCATAGTGGCCAACCCTGTGTCCCGGGACGCCCAGAGTT CTGTGTTGTTTGACGTCACAGGACAAGTCCGGCTCCGGCACGCTGACCAGGAGATCCGACTAGCCCAGGA CCCCTTCCCTCTGTACCCAGGGGAGCTGCTGGAAAAGGACATCACCCCGCTGCAGGTGGTTCTGCCCAAC ACTGCCCTGCATCTGAAGGCATTGCTGGACTTTGAAGATAAGAATGGAGACAAGGTCATGGCAGGAGACG AGTGGCTGTTTGAGGGACCTGGCACCTACATCCCACAGAAGGAGGTGGAAGTCGTGGAGATCATTCAGGC CACAGTCATCAAACAGAACCAAGCTCTGCGGCTCAGGGCCCGCAAGGAATGCTTTGACCGGGATGGCAAG GAACGGGTGACAGGTGAGGAGTGGCTGGTCCGATCCGTCGGTGCTTATCTCCCCGCTGTCTTTGAAGAGG TGCTGGATCTGGTGGACGCTGTGATCCTTACAGAAAAGACGGCCCTGCACCTCCGGGCTCGGCAAAACTT CAAGGACCTTCGGGGAGTGGCCCACCGCACTGGGGAGGAATGGCTAGTGACGGTGCAGGACACGGAAGCC CATGTCCCGGATGTCTATGAGGAGGTGCTGGGGGTGGTGCCCATCACCACCCTGGGACCTCGACACTACT GTGTTATCCTTGACCCAATGGGACCAGATGGCAAGAACCAGCTGGGACAAAAGCGTGTTGTCAAGGGAGA GAAGTCCTTTTTCCTCCAGCCAGGAGAAAGGCTGGAACGAGGCATCCAGGATGTGTACGTGCTGTCAGAG CAGCAGGGGCTCCTGCTGAAGGCGCTGCAGCCCCTGGAGGAGGGGGAGGGGGAGGAGAAGGTCGCCCACC AGGCTGGAGACCGCTGGCTCATCCGAGGGCCCCTGGAGTATGTGCCATCTGCCAAAGTGGAGGTGGTGGA AGAGCGTCAGGCTATCCCTCTGGACCAAAATGAGGGCATCTATGTTCAGGATGTCAAGACGGGAAAGGTG CGGGCTGTGATTGGAAGCACCTACATGCTGACTCAGGATGAAGTCCTGTGGGAAAAGGAACTGCCTTCTG GAGTGGAGGAGCTGCTGAACTTGGGGCATGACCCTCTGGCTGACAGGGGTCAGAAGGGCACAGCCAAGGT CCTTCAGCCCTCAGCTGCAAGGAACAAGACCCGAGTGGTCAGCTACCGTGTCCCCCACAACGCAGCGGTG CAGGTGTACGACTACAGAGCCAAGAGAGCCCGTGTGGTCTTTGGGCCTGAGCTAGTGTCACTGGATCCCG AGGAGCAGTTCACAGTGTTGTCCCTTTCTGCTGGGCGACCCAAGCGTCCTCATGCCCGTCGTGCACTCTG CCTGCTGCTGGGACCTGATTTCTTCACTGACGTCATCACCATCGAAACTGCAGATCATGCCAGGCTGCAG CTGCAGCTGGCTTACAACTGGCACTTTGAACTGAAGAACCGGAATGATCCTGAAGAGACAGCCAAGCTTT TCTCTGTGCCTGACTTTGTGGGCGACGCCTGCAAGGCCATTGCGTCCCGAGTCCGGGGGGCTGTAGCCTC GGTCACATTTGATGACTTCCATAAGAACTCAGCACGTATCATTCGCATGGCTGTTTTTGGCTTTGAGATG TCCGAAGATGCCGGTCCTGATGGCGCACTCCTGCCCAGGGCTCGAGACCGGGCCGTCTTTCCCCAAAATG GGCTGGTTGTGAGCAGTGTGGACGTGCAGTCAGTGGAGCCTGTGGACCAGAGGACCCGGGATGCCCTGCA GCGCAGTGTCCAGCTAGCCATCGAAATTACAACCAACTCCCAGGAGGCAGCAGCCAAGCACGAGGCTCAG AGACTGGAGCAGGAAGCCCGTGGTCGGCTTGAGAGGCAGAAGATCTTGGACCAGTCAGAAGCTGAAAAAG CCCGCAAGGAACTTTTGGAGCTTGAGGCGATGAGCATGGCTGTGGAGAGCACTGGTAATGCCAAGGCGGA GGCTGAGTCCCGGGCAGAGGCAGCGAGGATTGAGGGAGAAGGCTCTGTGCTGCAGGCCAAGCTAAAGGCG CAGGCGTTAGCCATTGAGACGGAGGCTGAGCTGGAGCGAGTGAAGAAAGTGCGAGAGATGGAGCTGATCT ACTCCCGGGCCCAGCTGGAGCTGGAGGTGAGCAAGGCACAGCAGCTCGCCGACGTGGAGGCAAAGAAGTT CAAGGAGATGACAGAGGCACTGGGCCCCGGAACCATCAGGGACTTGGCTGTGGCCGGGCCAGAGATGCAG GTGAAACTTCTTCAGTCCCTGGGCCTGAAATCCACTCTCATCACAGATGGCTCGTCCCCCATCAACCTCT TCAACACAGCCTTCGGCTTGCTGGGGCTGGGGTCTGACGGTCAGCCACCTGTACAAAAGTGACCTGGCGG CCCAGCCCCAAGGAAGGCTTGCTTTACCAGGTCCTCTGAAGCAGCCACTCTGTGCCTTAGGTAGACACTG ACTACACTGACAATGGATAAATAAAATGACAACTGTAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3584439 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3584439 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7233 same embryo
	EMAGE:7234 same embryo
	EMAGE:7231 same embryo
	EMAGE:7236 same embryo
	EMAGE:7235 same embryo
	EurExpress:euxassay_004411 same experiment
	MGI:4826517 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source