EMAGE mouse gene expression spatial database

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EMAGE:7234

Mios missing oocyte, meiosis regulator, homolog (Drosophila) ( MGI:2182066)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004401_01	euxassay_004401_02	euxassay_004401_03	euxassay_004401_04	euxassay_004401_05

euxassay_004401_06	euxassay_004401_07	euxassay_004401_08	euxassay_004401_09	euxassay_004401_10

euxassay_004401_11	euxassay_004401_12	euxassay_004401_13	euxassay_004401_14	euxassay_004401_15

euxassay_004401_16	euxassay_004401_17	euxassay_004401_18	euxassay_004401_19	euxassay_004401_20

euxassay_004401_21	euxassay_004401_22	euxassay_004401_23	euxassay_004401_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7234_wholemount_strong.wlz

7234_wholemount_moderate.wlz

7234_wholemount_weak.wlz

7234_wholemount_possible.wlz

7234_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7234_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6419
Entity Detected:	Mios, missing oocyte, meiosis regulator, homolog (Drosophila) ( MGI:2182066)
Sequence:	sense strand is shown >T6419 AGGCGGGGCTCACGGCGAGGAGGTCTGCTTCGGAGGCGGCAGGGTGGTTTGGTCGGGGGCCCAGCGGGTG GCCCGGGTCACCGCCCGCGCTTCGGCGGCCATCTTGCCCGCGCCCGTCTCCCGCGGCGGGAGGGGCGGCG TACAGGCCGGAAACGTCTGTGTGGCGACAGGCCACCTCCTCAGGGACATAGCAAGAATCTCAAGACATGT TTCAGAAGCTCCCTCTATTTGTGAGAATCTAGTGAATGGACCTGACTGGACTTTTTAAGCCCATCAATAA ACATGAGCGGTACCAAGCCCGATATTTTGTGGGCACCACACCAAGTTGATAGATTTGTTGTATGTGACTC AGAATTGAGCCTTTATCATGTGGAATCGGCTGTGAATTCAGAACTCAAAGCTGGATCGTTACGTTTATCT GAAGACTCTGCAGCGACATTGCTGTCAATAAACTCAGATACACCCTATATGAAATGTGTTGCGTGGTATC TCAATTATGATCCTGAATGTCTCCTAGCAGTTGGACAAGCAAATGGTCGAGTTGTACTTACAAGTCTTGG TCAAGATCATAACTCTAAGTTCAAAGATTTGATAGGAAAAGAATTTGTTCCAAAGCATGCTCGCCAATGC AATACCCTTGCATGGAATCCACTGGATAGTAACTGGCTTGCTGCTGGTCTCGACAAACACAGAGCTGACT TTTCAGTTCTAATATGGGATATCTGCAGCAAATATACTCCTGATATTGTTCCCATGGAAAAAGTGAGACT TTCAGCAGGTGAAGCTGAAACAACATTACTAGTGACAAAACCGCTGTATGAGTTAGGGCAGAATGACGCT TCTCTATCTCTGTGTTGGCTTCCACGAGACCAGAAACTTCTTCTTGCTGGCATGCATCGTAATCTAGCAA TATTTGATCTCCGGAACACAAGCCAGAAGATGTTTGTAAATACAAAAGCTGTTCAAGGAGTGACAGTAGA CCCTTACTTCCACGATCGTGTTGCTTCCTTCTATGAAGGTCAGGTTGCAATATGGGATCTTAGAAAATTT GAGAAGCCAGTTTTGACTTTGACTGAACAACCAAAGCCCTTGACAAAGGTCGCATGGTGTCCAACCAGGA CAGGCCTGCTTGCTACTTTAACAAGGGATAGTAATATTATTAGGCTGTATGATATGCAGCATACACCCAC TCCCATCGGGGATGAAACCGAACCCACAATAATTGAAAGAAGTGTGCAGCCTTGTGACAATTACATTGCT TCCTTTGCATGGCATCCAACGAGTCAAAATCGAATGATAGTTTCGACTCCCAACCGAACAATGTCTGACT TCACTGTATTTGAAAGGATATCTCTTGCCTGGAGCCCAATTACATCTTTAATGTGGGCTTGTGGTCGCCA TTTGTATGAGTGTGCAGAAGAAGAAAGTGATAACTCCTTAGAAAAAGATATAGCAACAAAAATGCGCCTT CGGGCTTTATCAAGGTATGGACTTGATACAGAACAGGTTTGGAGAAACCACATTTTAGCTGGCAATGAAG ACCCACAGCTCAAGTCACTCTGGTATACTCTGCACTTTATGAAGCAATATACAGAAGATAATGATCAAAA ATCTCCAGGCAACAAAGGATCATTGGTTTATGCAGGAATTAAATCAATAGTAAAATCATCTTTGGGAATG GTAGAAAGCAGCAGACATAATTGGAGTGGCTTGGATAAACAAACTGATATTCAGAACTTAAATGAAGAAA GGATTTTAGCATTACAGCTTTGTGGGTGGATAAAGAAAGGAACGGATGTAGATGTAGGGCCATTTTTGAA CTCCCTTGTGCAAGAAGGAGAATGGGAAAGAGCTGCTGCTGTGGCTTTGTTCAACTTGGATATTCGGCGA GCAATCCAAATCCTGAATGAAGGGGCGTCTTCAGAAAAAGGAGATCTGAATCTCAATGTGGTGGCAATGG CTTTATCGGGGTATACGGACGAGAAGAACTCGCTGTGGCGGGAGATGTGCAGCACCCTGCGCCTGCAGCT CAACAACCCCTATCTGTGTGTCATGTTTGCGTTCCTGACGAGTGAAGCGGGGGCATATGATGGAGTGTTG TATGAAAACAAAGTTGCTGTACGTGACAGAGTGGCATTCGCTTGTAAATTCCTTGGTGATGCTCAGTTAA ATAAATACATTGAAAAGCTGACCAATGAAATGAAAGAGGCTGGAAATTTGGAGGGGATCTTGCTTACAGG CCTCACTAAAGATGGAGTGGACCTGATGGAGAGCTATGTGGATAGAACTGGAGATGTTCAAACAGCAAGT TACTGCATGTTACAGGGTTCACCTTTAGATGTTCTGAAAGATGAAAGGGTTCAATATTGGATTGAGAATT ACAGAAATTTATTAGATGCCTGGAGGTTTTGGCACAAAAGGGCAGAATTTGATATTCATAGGAGTAAGCT GGATCCCAGTTCCAAGCCTTTAGCACAGGTGTTTGTGAGTTGTAATTTTTGTGGCAAATCAATCTCCTAC AGCTGTTCCTCTGTGCCTCATCAGGGCAGAGGGTTTAGTCAGTATGGTGTGAGTGGCTCACCAACAAAAT CCAAAGTCACAAGCTGCCCTGGCTGTCGGAAACCCCTTCCTCGATGTGCACTTTGCCTCATTAATATGGG AACACCTGTTTCTAGCTGTCCTGGTGGATCCAAATCAGATGAAAAGGTGGACTTGAGCAAGGACAAAAAG TTAGCTCAGTTTAACAACTGGTTTACCTGGTGTCACAACTGTCGGCATGGTGGGCATGCCGGACATATGC TGAGTTGGTTCAGGGACCATGCAGAGTGTCCTGTATCTGCATGCACGTGTAAATGTATGCAGTTGGATAC AACAGGAAATCTGGTGCCAGCAGAGACTGTCCAGCCATGAAATGTTCCACCTAAAGAAAAATCTTCACAT GTGGAGTTTTTAAAGGATGTCCTTCATAGTTCAGGAAATAAAGTCACTCATGGCTTTGCTGTCGTGGAAA AATAAAGCATTCTGTCAGCTCAGCAATGTGATGTTTGAGTGACTTTGACGTACTCCTCAGAGGCCAACGC TGCTGATAGGAACATTAGAACATGAATATTGGTGCTGTTTAGTAGGTTAAAGTTCATTTGAAAGAACTGT CTAGGCTTTTGTCAAAATTATGTTTATAGATGGTTTTAGAATAAAAACAAGCCTTGTGCCTGCATCTTCA ATCTGAAATTGATATCTTTTGATAAAATCTGTACATATCTAAAACCAAATGCCAATTCATTGTTTCATAT ATCAATTTAAGCTTATTCATGCTTAAATATGCTTTCAGAGAAAATGTCAATTTTACATTGGTAATTAGCT CCTGGCCCATGCTGGAGAATACATGCATGTGTGCATATGTGCACACACACGTTTAAGGAACGTTCTCCCT CATACATTTATATTTTAATATAAAAGATTTGACATAGGTCATTCTTGTCTTCCTCAAAACTCCAAGTTAA AAGCAGGCTGGTGAGCTGGTTCAGCATGCGAGGAGGCTTGCAGCCAATCCTGATGACCTAAGTGCGGTCC CATGTGTTGGAAGCAGTGATTCAACTCATCTCCAGAACCTTGCTGCAGCATGGGGCGAGCACGTACACAT GTGCGCGCTCGTGAGCACTCGAGCACACGCAGACAATAAATAAAAAAAATTTAAAACGATAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:3584335 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3584335 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7233 same embryo
	EMAGE:7232 same embryo
	EMAGE:7231 same embryo
	EMAGE:7236 same embryo
	EMAGE:7235 same embryo
	EurExpress:euxassay_004401 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source