EMAGE mouse gene expression spatial database

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EMAGE:7286

Slc17a6 solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6 ( MGI:2156052)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004371. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004371_01	euxassay_004371_02	euxassay_004371_03	euxassay_004371_04

euxassay_004371_05	euxassay_004371_06	euxassay_004371_07	euxassay_004371_08	euxassay_004371_09

euxassay_004371_10	euxassay_004371_11	euxassay_004371_12	euxassay_004371_13	euxassay_004371_14

euxassay_004371_15	euxassay_004371_16	euxassay_004371_17	euxassay_004371_18	euxassay_004371_19

euxassay_004371_20	euxassay_004371_21	euxassay_004371_22	euxassay_004371_23	euxassay_004371_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7286_wholemount_strong.wlz

7286_wholemount_moderate.wlz

7286_wholemount_weak.wlz

7286_wholemount_possible.wlz

7286_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7286_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 13 14 15 16 17

diencephalon lateral wall mantle layer

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 21 22 23 24

olfactory cortex marginal layer

strong

regional

strong expression: see section 07 08 09 10 13 14 15 16

medulla oblongata alar plate mantle layer

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

medulla oblongata basal plate mantle layer

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

rest of cerebellum marginal layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21

pons mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain mantle layer

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

strong

regional

strong expression: see section 03 04 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

strong

regional

strong expression: see section 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 17 18 19

spinal cord

strong

homogeneous

strong expression: see section 09 10 11 12 13 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 13 14 15 16 17 18

not examined

regional

not examined expression: see section 01 02 21 22 23 24

neural retina

strong

regional

strong expression: see section 01 02 21 22 moderate expression: see section 23 24

nasal cavity olfactory epithelium

moderate

single cell

moderate expression: see section 06 07 08 09 10 11 13 14 weak expression: see section 15 16 17

tail mesenchyme

moderate

regional

moderate expression: see section 09 10 11 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5235
Entity Detected:	Slc17a6, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6 ( MGI:2156052)
Sequence:	sense strand is shown >T5235 AGCTATTCTGTTGTACTCTCTTTCTCGCCCTCCCTCCCCTCTCCAACTCACAGCCTTGCTGGAAAGCTCA CCTCTGCTGAGAAGAAAAAGCTCTACCTTAACCAACTAAGACTATGCGCAGAATCCGTCTTTCATAGCCA CAACAATTTAAATCTGGTAAGGCTGGACACCAGTCTTTACAAGAATGGAGTCGGTAAAACAAAGGATTTT GGCCCCGGGGAAAGAGGGGATAAAGAATTTTGCTGGAAAATCCCTCGGACAGATCTACAGGGTGCTGGAG AAGAAGCAGGACAACCGAGAGACCATCGAGCTGACAGAGGACGGTAAGCCCCTGGAGGTGCCTGAGAAGA AGGCTCCGCTATGCGACTGCACGTGCTTCGGCCTGCCGCGCCGCTACATCATAGCCATCATGAGCGGCCT CGGCTTCTGCATATCCTTCGGCATCCGCTGTAACCTGGGCGTGGCCATCGTGGACATGGTCAACAACAGC ACTATCCACCGCGGAGGCAAAGTTATCAAGGAGAAAGCCAAATTTAACTGGGACCCCGAGACCGTGGGGA TGATCCACGGATCGTTCTTCTGGGGCTATATCATCACCCAGATTCCAGGAGGATATATCGCATCGCGGCT GGCTGCTAACCGGGTCTTTGGGGCTGCGATACTGCTCACCTCTACCCTCAATATGCTGATCCCATCTGCA GCCAGAGTGCATTATGGATGTGTCATCTTTGTTAGGATATTGCAAGGACTTGTGGAGGGTGTCACCTACC CAGCCTGTCATGGGATATGGAGCAAGTGGGCCCCTCCCTTGGAGAGGAGTAGGTTGGCTACAACCTCCTT TTGTGGTTCCTATGCTGGAGCAGTCATTGCAATGCCCTTAGCTGGTATCCTTGTGCAGTACACTGGATGG TCGTCAGTATTTTATGTGTATGGAAGCTTTGGCATGGTCTGGTACATGTTCTGGCTTCTGGTGTCTTATG AGAGCCCTGCAAAGCATCCTACCATTACAGATGAAGAACGTAGGTACATAGAGGAGAGCATTGGAGAGAG CGCAAATCTGCTAGGTGCAATGGAAAAATTTAAGACCCCATGGAGGAAGTTTTTCACATCCATGCCCGTC TACGCGATAATTGTTGCCAACTTCTGCAGGAGCTGGACTTTTTATTTACTGCTCATCAGTCAGCCAGCTT ATTTTGAGGAGGTTTTTGGATTTGAAATCAGCAAGGTTGGCATGTTGTCTGCAGTCCCTCACCTTGTCAT GACAATCATTGTGCCTATCGGGGGGCAAATTGCAGATTTCCTAAGGAGCAAGCAAATTCTCTCAACAACT ACAGTGAGAAAGATCATGAATTGTGGGGGTTTTGGCATGGAAGCCACGCTGCTTCTGGTTGTTGGCTACT CTCATACTAGAGGGGTGGCCATCTCCTTCTTGGTGCTTGCAGTAGGATTCAGTGGATTTGCTATCTCTGG TTTCAATGTTAATCACTTGGATATTGCTCCAAGATATGCCAGTATCTTAATGGGCATTTCAAATGGCGTT GGCACGCTGTCGGGGATGGTTTGCCCTATCATTGTTGGTGCAATGACAAAGAATAAGTCCCGTGAAGAAT GGCAGTATGTCTTCCTCATTGCTGCACTCGTCCACTATGGTGGAGTCATATTTTATGCACTATTTGCCTC AGGAGAGAAACAACCTTGGGCAGACCCTGAGGAAACAAGCGAAGAAAAATGTGGCTTCATTCACGAAGAT GAACTGGATGAAGAAACGGGGGACATCACTCAGAATTACATAAATTACGGTACCACCAAATCTTACGGTG CTACCTCACAGGAGAATGGAGGCTGGCCTAACGGCTGGGAGAAAAAGGAAGAATTTGTGCAAGAAGGTGC GCAAGACGCGTACACCTATAAGGACCGAGATGATTATTCATAACGATGCTAGTTGCTGGATTCATTTGTA GTGTTTGTGAATCAATTAATTGTGATTGCACAAAAATAATTTTAAAAATGTGGTGTGAACATGTAAACAT ATCAACCAAGCAAGTCTTGCTGTTCAAAAACAAAAACAAAAAAATCTGAATTCAAAACAGACCATGAGAT TCCCATCAAGTGCAATCTGTGGCAGTTGTCACGTTATGCCGTCTTCATTCAGGCCATTTGTCCTTTCGTT TGTGATTTAAAGGTTTCCTGTAGAAATAAGTAGGTATTCGTTGGACCCATCACCATTTTAGAGAGCACAA CTACAACAGTTGGCACATGTCATCCTACAGAAGTTAGGAAGCCAAAGCTACTGGATCATGCAAACTGCAC TTATTTATTACACTGGACTGCAAACTATCCCAGGGAAAGCCTGTCTAGAGACATAGTGGAACAGGAAAGA TGGCTAGATCAGGTATTGACTATAATCATTATGTGTCTATCATGGAGTGGCTATATCTTTCAATGAAGAA CTATATTGTGTAGCTAGCAAACTGTACTGAACTCTTACTAGGAGTGCACAGTGTGTGATATTTTGTGATC TTCCAAAAGCTTATCTTGCAGTGTTTTGTGAAATGCTTGGGCACAAACACTTATTTTTATGAATGAGAGC TTGTAAAGGGAGGGGTATGCTCCATGCTCCCCCATTCACTACCTGACAGTATCAAACCCTTCACATTTCA ATAAAATCCAACTTTCATGTAACATATCACATAACTTTTTTTTTTTGCAAAAAAATAAGAAGAAATAGAC TTCAATGTATTTTTTATTACAACTTTGTACTGGTTGTAACTTGCATTAGGAAAAAATGATTAATATATGT ATAATCATAAAGAATCTAATAAAAATTTACTATGATGATATAGCCCTTAAAATGCAATATTAACAAAATA AATAGAAAATTTAGATAATCTTCCTTGATAACTAGAGACTATATGAAACTCATGCCACAAAGCTATATAT AATATGAAGAGATAAACAATAGAGATTCTATATGTAGACAATTTTATTACCTAATGTCCCACATAAGAGA TATTTGTCTTGAGTATATAGTACAAAATATATTAAAATTATATCTACATCCCTGTATATCTTATACATAT CCACTCACACGAACATAACAAATACTTTTCACACAGAACCAAAAACAAGCATACACCTAATGTTGGGTTT GGGGATTGCAATTTCTACTTTCATAGAGTCATAGAATTTTAGATGGAAAAAAAAAAGCATTTTGCTCGTC ATTTCTTAATATAATTCAACAGGAACTGCAACATTTGTGTACCAAGCAATAAGTGCAAAGCATAAAACCT CCTGTGTGTAAATTATCCCCATGTTGCTTGTGGTAGCAGTGATTTTTTTTTTTTAAAAAAGTTAACCTCA GAGCTCTTTTTAATGTTTTGCGCTGATAAGAATGCACATCCCAATTTACTGCAAAATGTCACCTGGTGTG TTTACCTGTCAGTTTTGGGTATTTGATCTGTCTGGTGTCCTGTGCTCTTGACTGGAGGCCCTGCTACTGC AAATATACAATGTGAAGTTTGTTTTTAAATGCAAACCACTCCTGACCTTAAGAAACTGAAGTCCCTCTCT GCTTTGTGTCTCAGAGTACTATCATGTGACCATAACCTTTGCTGTGCTGAGTAAAGAGATGTGAACTGTC ATTTTGTTGCTGCAAAACAAGTGTTAATAAAATGTTCTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5357143 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5357143 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7288 same embryo
	EMAGE:7289 same embryo
	EMAGE:7290 same embryo
	EMAGE:7285 same embryo
	EMAGE:7287 same embryo
	EurExpress:euxassay_004371 same experiment
	MGI:4828119 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source