EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7288

Prr11 proline rich 11 ( MGI:2444496)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004322. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004322_01	euxassay_004322_02	euxassay_004322_03	euxassay_004322_04

euxassay_004322_05	euxassay_004322_06	euxassay_004322_07	euxassay_004322_08	euxassay_004322_09

euxassay_004322_10	euxassay_004322_11	euxassay_004322_12	euxassay_004322_13	euxassay_004322_14

euxassay_004322_15	euxassay_004322_16	euxassay_004322_17	euxassay_004322_18	euxassay_004322_19

euxassay_004322_20	euxassay_004322_21	euxassay_004322_22	euxassay_004322_23	euxassay_004322_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7288_wholemount_strong.wlz

7288_wholemount_moderate.wlz

7288_wholemount_weak.wlz

7288_wholemount_possible.wlz

7288_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7288_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5089
Entity Detected:	Prr11, proline rich 11 ( MGI:2444496)
Sequence:	sense strand is shown >T5089 CCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGGGAAGAGGCTTAGGCCCGAACTTCGTCTAATCCCC AGGCTCTTAGACTAAAATCATGCCCAAGTACAAACAGCGAAGAAGAAAGCTAAAAGCCAAAGCCAAACGA ATGTCTAAGAAGAAAGAAGCCGCTGTGGTCTCTCCTAAGTTATTGACACCTTCCCCTCCCCTCCCCGAAC CGGAAAGAGTGGTTACTTCTGCAGCAGATATTCCCCAAAGCAGAAACTGGCTAAGACCATCTTGGAACCT GAGATTTCCTAACATCAAAGATGCAATAAACCTTTGGACAAATAGAGCATGGTGCATCTACAGTTGCTGT CAGACCTGTGTAGCCCAGAGTTTAGAAGTATTGAAAGATGCCCTCTTCCCATCCCGAGTCTACCACAGAG AACTTCACAGTCTAAAACAACAACTGTGTGTTTTGAAAAGGGAATTGTGCAAGCTCCGAGAAAACCTGAA GAGCATCTCAGAAAACTCCTCCTGTTCAAGCTGTTGTCACAAGTGTTGCCCGAGTGATAAACTTACAACT GTGCCTGCCTGTGCTCCAACGACGAATGGGGAATCCCAAACAGTGCTTTCTTCCACACAGCCACAGCCAG CCAATCATCCTCCTTCTCCTCCTCCCCTTCCTCCCCCTCCTCCCCCTCCTCCTCCTCTCCCTCCACCTCC ACCACCTTTAGCACCTTTGCTGCTCAGAAAATCTGGTACCACTAAAGCACTTCAGGTTGAACCATTAAAG AAAGATGGACCCATGCATATAACAGTTAAAGATCTACTCAATGTGAAATTAAAAAAGACACAGAGTGTTG ACGAAAGAAAGAAGCTTGTACCATCACCACCAGAGGAACGCACTCCACTAGTTACTGTCTCTGATCTGCA ACATGTTACTCTGAAACCTAACTCCAGGGTGTCAGCAACTCGAATTAAAAATGTCTTAATTACTCCTGGG AAAAGCCAAATCGATCTCCGGAAGCTGCTGAGAAAAGTCCAGGTAGAAAGGAGCCCGGGTGGGACCCCTC TGAACAATAAAGAAAACATGGATACAGGAACTGGACTCACTCCTGTCATGACTCGGGCCTTAAGGAGAAA GTTTCAGCTGGCTCATCCTCAAAGCCCCACTCAATCTCTGCCACTTTCTACAAGCAGCTTTGATGAACAA AACTGAGAGCAACTCGGCCTGACTCCCGGGAGTGAAACATACAATGAAAGACCAACCGCCCAAATGTTAA TGTACTAAAATATGTGTAACTATGAGTAATTCATCTGAAAGACTCAGAATATGTCTGGAGCCTACACATA GTGTAGCATAAAAGCATTTAGATATTTTCTGGCGTGGGGTAAGGGGACCAGATTAGAAAATTCAGTCTGC AAACTAATGTTTATCCAGAAATTACCCAATTTAGGCTACAGTAACTGTTCAAATTTAATTCCATGGGAGC TGGCAATATGGCTCAAAGGATAAAGACACTTGCCACCAAGCCTAGTGACTCAAGTTCTATACCCAGGACC CAAATGGAGGGAAGAAAAGAACTATGATGTGTGCCCCTTCAACACACAAAATTAATATATATGTTAGGAA ATACTAACGGTATAAATAATTTGTTATATAATGGAAGATATGACATTAAGAACATAGTCTGAGTCTTCTT TTTCTTCCTCCTTCTCTTCTTCCTCCTCTTCCTTCTTCTCATCCCTCTGCTTCCTCTCTTTGGGGCTGAA CCCAGGGCCTCACACATGCTAAGTACATACTCCCTGAAATATACCTTCAGCCTTTAGTGCTTGCTATTAT ATCTGCTGTCCATGTTCCAGCAGTCAAATAAATTCTTCATGGTTTCAGCATGAACTCTCTATTTTCCAGA ATGCTTTGTCAAGGATCCTCTGTGGTCTGTGGCAGTTTTATCAGGAAGCCTGGTCCCCGCTTGGCTCCTT ACCAGCCTGCGTATTCTGGCAGTGCTGTAGCTCCCACTGGACTTCAGGCTTGCTAGTCTAAGGCTGTCAG ACTTAGCAAATGACAGGATAAAGTACTCCATTAAATTTGAATTTCAGATAAATCACACACAAAAAAGCTT TAAGAATAAATCTGCCCTACTAAACAACAAATATTTGAGTGTTACATAGAACTCACGGCCAAGAATGTGT TTAACTAGTAGAAATATCTCCTTAATATTGAATGAGGTACACTTACATTTAAAAACTACATTTAATTCTT TGCAGGAGTTCAGGGATATAGTGCTTCTCTCGAATGTTCACGGCCCTAGGTTCAAACCCCAGTACTAACA CACAAACGTATGCATACACACACTCATTCATGTGATGGTTAATATCAGTTATATACGTCATGTTATCTAT AATTACCTGGAAGATGGCTTTCCATCTTGATTGGGTTAATTGAGATGGGATAACCTACCCTCAGGGATAG ACTACCCTGAGCTGTGCATGCAGCTCTTGATTCCTTCCCTTCCACAACGGAGTGTGTGGTAGAACTATAA GCCAAAGGAACCCTCCCTTTTCTCAGCTATTTCATCACAGTGACGACAAAACTAACTGGTCATTCATCAT ATCTGTGTATATATGTCTTATTCTCTGAAATTCACACTTAACTGAGAACCTTATATTTTACCTAGCTACC TTGTTTGTCCATTCTTGAAAAATTGCAATTTATTTCTGGAATCCCATGGTTTACTACATGTCTTCATGGC ACTGAACACTATGTAGCTTCAGCCAGGAAGCCATCACATATGTTCTAAGGGGAGTAGATGGATCATTTCG ATTAAAGTGAGTATCGCTCAAGATGCAGAAGTAGCTGTCTTTCTTTTCCAGTGCAGAGGAGCCTCGGGGC TTTGAGCACGCTGGGCAAGTGCTTCTTGCAAGTGATCTGAGCCATACTCCCAGCTCCAGCATCACCTCTG ATGCTGTTATTGCACAATGGTAGGAAAGAGACAGAGCATAGAGGTGTATTTCCAGAAATCCCATTATTTC TATTGAATGATAGTGCTTTGAGATGCCAAATGGTGGTGTTGGAAAGTCATCAGACTGGAAAGTGGTCCTG GGGACAGTGTTTAGTACCTAAGAGGCAGTCAGTTTAAATAAAGTAAGGGCTTTTAGTAGAATGTCATTTT GTGGGCAGCTATAATGTTTACAATGGTAATGAACCACTATATACTGTACATTTGATCATTCTTCTGGCAT AAAAGGCTCGTTTAAAACAAAACAAAACAAACAAACAAACTAGGATGGGCATGGTGGCTTTTGCCAGTTG TCCCAGTTACTTGGAAAACTGAGGTGGAGGGAAGCCTGGGCAACTTGGTAAGAACCTGCCTCAAAAAAAA ATAGAAATCTTACAGGTAAAACAGTTATAGGGACTGGAGAGATAGCTCAGCAGTTAAGAGCACTGACTGC TCTTCCAAAGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCTGTAACGAGAACTG ATGCCCTCTTCTGGTGTGTCTGAAGATAGCTACAGTGTACTTACATACAATAAATAAATCTTTTTAAAAA ATTTTTATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5323297 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5323297 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7289 same embryo
	EMAGE:7290 same embryo
	EMAGE:7285 same embryo
	EMAGE:7287 same embryo
	EMAGE:7286 same embryo
	EurExpress:euxassay_004322 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source