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EMAGE:7290

Sipa1 signal-induced proliferation associated gene 1 ( MGI:107576)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7290 EMAGE:7290 EMAGE:7290 EMAGE:7290 EMAGE:7290
"Pseudo-wholemount" of euxassay_004345. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004345_01 euxassay_004345_02 euxassay_004345_03 euxassay_004345_04
EMAGE:7290 EMAGE:7290 EMAGE:7290 EMAGE:7290 EMAGE:7290
euxassay_004345_05 euxassay_004345_06 euxassay_004345_07 euxassay_004345_08 euxassay_004345_09
EMAGE:7290 EMAGE:7290 EMAGE:7290 EMAGE:7290 EMAGE:7290
euxassay_004345_10 euxassay_004345_11 euxassay_004345_12 euxassay_004345_13 euxassay_004345_14
EMAGE:7290 EMAGE:7290 EMAGE:7290 EMAGE:7290 EMAGE:7290
euxassay_004345_15 euxassay_004345_16 euxassay_004345_17 euxassay_004345_18 euxassay_004345_19
EMAGE:7290 EMAGE:7290 EMAGE:7290 EMAGE:7290 EMAGE:7290
euxassay_004345_20 euxassay_004345_21 euxassay_004345_22 euxassay_004345_23 euxassay_004345_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7290Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7290_wholemount_strong.wlz
7290_wholemount_moderate.wlz
7290_wholemount_weak.wlz
7290_wholemount_possible.wlz
7290_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7290_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5099
Entity Detected:Sipa1, signal-induced proliferation associated gene 1 ( MGI:107576)
Sequence:sense strand is shown

>T5099
GCGGGACCTTGGTGCGGAAGGCAGCGGTGGCCAGCTTGAGCCCGAGAGCAGCTGCCCCAGGAGCTCCTGT
GTCCTTGAGGCCCATCTGAACAGCCCCCTCCTCCTGCAGTGCAGGAACCACTGAAGCCTCAGCCTTCTGG
CTGGGCACCAAGGACCCGTGCCCACCAATGCGGCCCGGCCCCCAGAGAGTCAGGCCCACAGAGCACGCCC
ATGTGGGCCGGCGGTGTGGGGAGCCCTCGGCGGGGCATGGCCCCTGCACCTACCGATGACCTCTTTGCCC
GTAAGCTTCGCCAACCTGCCCGGCCCCCACTGACACCACATACCTTTGAGCCGAGGCCAGCTCGGGGCCC
ACTCTTGCGCAGTGGCAGTGATGCTGGTGAAGTCCGGCCCCCTACACCAGCCAGCCCCCGTGCCCGTGCC
CACAGCCACGAGGATGCCAGCCGCCCTGCTGCAACCCCTACTCGGCTCTTCACTGACCCACTGGCACTGC
TAGGGTTGCCAGCAGAAGAGCCAGAGCCCACCTTCCCGCCAGTGCTGGAACCCCGGTGGTTTGCTCACTA
TGATGTGCAGAGCTTGCTCTTTGACTGGGCTCCACGACCTCGGGGGACAGGCAGCCATACAGAGGCAAAC
TCTGGGACCTTAGCTGAGGGCCAGACTACCACCTCAGATCTACTGCTCGGGGCACCTGGCTTTGTGAGCG
AGCTTGGTGGTGAGGGTGAGCTAGGGCTGGGTGGGCCAATATCCCCACCTGTGCCCCCTGCACTGCCTAA
TGCGGCTGTGTCCGTCCTGGAGGAGCCACAGACCCGGACCACAGCTTACAGCCTGGAGCACGCAGATCTG
GGTGCAGGCTACTACCGCAAGTACTTCTATGGCAAAGAACACCAGAACTTCTTTGGGTTGGATGAGGCGC
TGGGTCCGGTGGCCGTGAGCCTGCGACGGGAGGAGAAAGAGGGCAGCGGAGGGGGCACCCTACACAGCTA
CCGGGTCATCGTGCGGACCACGCAGCTCCGGACCCTCCGTGGCACCATCTCGGAGGACGCACTGCCTCCC
GGCCCCCCGAGGGGCCTATCTCCGAGGAAGCTTCTGGAACATGTGGCTCCACGGCTGAGCCCCACCTGCC
TGCGCCTGGGTTCAGCCTCTCCCAAGGTGCCCCGCACGCTGCTTACTCTGGATGAGCAAGTGCTGAGCTT
CCAACGCAAGGTGGGCATCCTGTACTGCCGCGCAGGCCAGGGCTCTGAGGAAGAGATGTACAACAACCAG
GAGGCCGGAGCAGCCTTTATGCAGTTCCTTACTTTGCTGGGTGATGTGGTGCGACTCAAAGGCTTTGAAA
GTTACCGGGCCCAGCTGGATACCAAAACGGATTCCACGGGCACACACTCACTCTACACCACCTACCAAGA
CCATGAGATCATGTTTCACGTGTCCACGATGCTGCCTTACACGCCTAATAACCAGCAACAGCTCCTGAGG
AAGCGTCATATCGGCAACGATATTGTGACCATCGTGTTCCAGGAGCCCGGTAGCAAGCCCTTCTGCCCTA
CAACAATCCGCTCTCACTTCCAGCACGTTTTCTTGGTGGTGCGTGCGCATGCTCCCTGCACCCCACACAC
CTCATACAGGGTGGCAGTGAGCCGCACCCAGGACACTCCTGCCTTCGGGCCTGCGCTGCCAGAAGGCGGA
GGCCCCTTTGCAGCCAATGCCGATTTCCGGGCCTTTCTGTTGGCTAAGGCACTCAATGGTGAGCAAGCGG
CTGGTCATGCACGCCAGTTCCACGCCATGGCTACACGCACACGCCAACAGTACCTGCAGGACCTGGCTAC
TAATGAAGTGACCACTACTTCGCTGGACTCGGCTTCGCGGTTTGGCCTGCCATCTCTGGGGGGTAGGCGC
CGGGCAACCCCTCGGAGCCCAGGCGCGGAGCTACAGGCGGCGGGTGCGCTGATGTGGGGCGTACGCGCGG
CTCCAGGGGCGCGGGTCGCAGCGGGAGCTGAAACGAGCGGTCCGGACGACGCCGAGGTGCCCTGCTTGTT
GGGCATCTCAGCAGAGACACTGGTGCTGGTGGCACCTCGCGACGGCCGCGTGGTCTTCAATTGTGCCTGT
CGCGACGTATTGGCCTGGACCTTCTCAGAGCACCAACTCGATCTGTACCACGGGCGCGGGGAGGCGATCA
CGCTGCGGCTCGACGGGGCCCCAGGGCAAGCCGTGGGCGAAGTCGTGGCACGTCTGCAGCTGGTGAGCCG
CGGGTGTGAGACCAGAGAACTAGCGCTGCCCAGAGATGGCCAAGGTCGCCTGGGCTTCGAGGTGGATGCA
GAAGGCTTCATCACGCACGTGGAGCGCTTCACGTTTGCGGAGACCACGGGGCTTCGGCCTGGAGCTCGTT
TGCTGCGAGTCTGCGGCCAGACGCTGCCCAAGCTGGGTCCCGAAACTGCTGCCCAGATGCTGCGCTCTGC
GCCCAAGGTCTGCGTCACGGTCCTACCCCCAGACGAGAGCGGCCGGCCGCGAAGGAGCTTTTCGGAGCTC
TATATGCTCTCTCTGAAGGAACCCAGCCGGCGGGGGGGCCCAGAGCCAGTACAGGATGAAACTGGGAAGT
TGGTCATATTGCCTCCCACCAAGCAGCTGCTACATTTTTGCCTGAAAGACAGCAGCAGTCCTCCGGGGCC
TGGGGATCTGACTGAGGAGAGGACAGAGTTCCTGCGCAGCCACAACTCCCTGTCATCTGGAAGCTCCCTG
TCCGATGAGGCTCCAGTCCTGCCCAACACCACTCCAGACCTCCTCCTTGTCACCACTGCCAACCCATCTG
CACCTGGTACTGACAGAGAAACACCCCCTTCCCAGGACCAGTCAGGAAGCCCCAGTAGCCATGAAGACAC
CAGTGACTCAGGCCCAGAACTGAGGGCCTCCATCCTGCCCAGAACCTTGTCTCTGCGGAATTCCATCAGT
AAGATTATGTCGGAAGCTGGCAGTGAGACCCTGGAGGATGAGTGGCAGTCCATCTCAGAGATCGCCTCCA
CTTGCAACACAATTCTGGAGTCACTGTCCCGGGAGGGACAACCCATCTCAGAGAGCGGAGACCCCAAGGA
AGCTTTAAAGTGTGATTCTGAGCCAGAACCCGGGAGCCTGTCAGAAAAGGTCTCTCACCTAGAGTCCATG
CTCTGGAAGCTCCAGGAGGACCTGCAGAGGGAGAAGGCGGACAGGGCAGCCTTGGAGGAGGAGGTTCGGA
GCCTCAGACACAACAACCAGAGGCTGCTGGCAGAGTCCGAGAGTGCCGCCACCCGCCTGCTCCTGGCCTC
TAAGCATCTGGGTGCACCCACTACTGACCTGGCCTGAGTTCCAATCTGAATCTGGACCTGCTTGGAACTG
CCTGGCCCCTCAGAGCAACTGGGTCATACTAGTGCCCTTCCTCAGGACTTCTTCCCTGCGCTGAGGCGCG
TCTTAGCACTGCCCCCTCTTCCCAGCCCATTTGGTGGCTAATGCCTGTCCCTGTTTGTAAATATCCTGTA
AAGAAAAGGAGACATCAGAGTTTAAAAAAAAGAAACAACAAGAAGAAGCAAACAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5324326 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5324326 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7288 same embryo
 EMAGE:7289 same embryo
 EMAGE:7285 same embryo
 EMAGE:7287 same embryo
 EMAGE:7286 same embryo
 EurExpress:euxassay_004345 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS