EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7360

Naaa N-acylethanolamine acid amidase ( MGI:1914361)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004482. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004482_01	euxassay_004482_02	euxassay_004482_03	euxassay_004482_04

euxassay_004482_05	euxassay_004482_06	euxassay_004482_07	euxassay_004482_08	euxassay_004482_09

euxassay_004482_10	euxassay_004482_11	euxassay_004482_12	euxassay_004482_13	euxassay_004482_14

euxassay_004482_15	euxassay_004482_16	euxassay_004482_17	euxassay_004482_18	euxassay_004482_19

euxassay_004482_20	euxassay_004482_21	euxassay_004482_22	euxassay_004482_23	euxassay_004482_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7360_wholemount_strong.wlz

7360_wholemount_moderate.wlz

7360_wholemount_weak.wlz

7360_wholemount_possible.wlz

7360_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7360_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 13

choroid invagination

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

telencephalon ventricular layer

moderate

regional

moderate expression: see section 16

4th ventricle

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15

spinal cord ventricular layer

moderate

regional

moderate expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6461
Entity Detected:	Naaa, N-acylethanolamine acid amidase ( MGI:1914361)
Sequence:	sense strand is shown >T6461 GGCCTCAGCAGCCGGAGCTATGGGGACCCTAGCCACCAGGGCCGCGTGCCATGGGGCACACCTGGCCCTG GCTCTGTTGCTGCTACTGTCGCTGTCCGGTCCCTGGCTGTCGGCTGTCGTCCCCGGGACTCCGCCTCTCT TCAACGTCAGTCTGGACGTGGCCCCGGAGCAGCGCTGGCTGCCGATGCTGCGGCACTACGACCCAGACTT CTTGCGCACCGCGGTGGCGCAGGTCATTGGCGACAGGGTTCCCCAGTGGGTACTCGGGATGGTCGGAGAA ATTGTGTCGAAGGTGGAGAGCTTCCTGCCTCAGCCCTTCACCGACGAGATCCGCAGCATCTGTGACTCGC TCAACCTCAGCCTGGCTGACGGTATCTTGGTCAACCTGGCCTACGAGGCTTCCGCATTCTGCACCAGTAT TGTGGCCCAAGACTCCCAAGGCCGTATTTACCACGGGCGGAACCTGGACTATCCTTTTGGAAAAATCTTA CGAAAGCTGACAGCGGACGTGCAGTTTATAAAGAATGGGCAGATTGCATTCACTGGGACCACTTTTGTTG GCTATGTAGGACTGTGGACAGGCCAAAGTCCACACAAGTTTACAATTTCTGGTGATGAACGAGATAAAGG CTGGTGGTGGGAGAATATGATCGCGGCGCTTTCTCTGGGACACTCCCCCATCAGCTGGCTTATCCGCAAA ACCCTGAGTGAGTCAGAAAGCTTCGAAGCAGCTGTCTACACGCTGGCCAAGACTCCCCTCATTGCTGACG TTTATTACATTGTTGGGGGTACATCGCCCAAGGAGGGAGTAGTCATCACCAGGGACCGAGGTGGCCCGGC AGACATTTGGCCTCTTGACCCTCTGAATGGAGAGTGGTTCCGAGTTGAGACAAATTATGACCATTGGAAG CCTGCACCCAAGGTGGATGACCGAAGAACACCAGCCATCAAAGCCCTAAATGCTACAGGGCAAGCTCACC TCAATCTGGAGACCCTCTTCCAGGTTTTATCCCTGTTTCCTGTTTATAACAACTACACAATTTATACTAC AGTGATGAGCGCTGCCGAGCCCGACAAGTACCTGACCATGATCAGAAACCCGAGCTGAAGGTGATGTATT GTCAAAAAGCTGCATTTAGAAGTCTAAGACTGGAAGGACTGTCCCGTTTCCTGAGCAACGCAGGCTCCCT CCCCACAGAGCGTTTCTGCCTGTGGAGGAAGAGGAGCGCAGCCTGCTGTCTTGGTCCTCCTGACTTCCTG GTCCCCTTGAGCTTCACTAACAGCCCACCTTGGCTTGAACAACTCATATAACTGGTCTCAGAGGTCAGGC TGCACCCAACCAGTCACAAGTGTCCTGTGATTCTGTGTTCACACTCCTGCTTGTCCACTGAAGAAGGGAC AGGACAAGATGGCTGTCATGTGGACAAACCCAGGTTTCTCCTAAGGCTGGGTGAGGCTTAGTGGCAGGGT TTGCACCGATAGATAGATAGATGATAGATAGATAGATAGATAGATAGATAGATAGATAATAGATAGAAAG ATAGATAGACAAATGAGAGTTTTCTCTGAAGAGCAGAGACCTCTAATTTGAGTAAAATTTTCTTAAAGCT ATGAACTCTGACTTTTGATTTTGTGTTTCCATTTAGTAGAAACTCATTTTTTGTAAACTTCTAATGTATT TTTTTAAATGTAAGCCTTATTAAAAATAAAACTACTTTCTTACTACTTTTGAAAAATATATGACACCAAA TAATATCCTGTCATCGTTTGTTAATAAAATGTCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3586255 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3586255 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7362 same embryo
	EMAGE:7361 same embryo
	EMAGE:7364 same embryo
	EMAGE:7359 same embryo
	EMAGE:7363 same embryo
	EurExpress:euxassay_004482 same experiment
	MGI:4826581 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source