EMAGE mouse gene expression spatial database

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EMAGE:7363

Tgfb1 transforming growth factor, beta 1 ( MGI:98725)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004507. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004507_01	euxassay_004507_02	euxassay_004507_03	euxassay_004507_04

euxassay_004507_05	euxassay_004507_06	euxassay_004507_07	euxassay_004507_08	euxassay_004507_09

euxassay_004507_10	euxassay_004507_11	euxassay_004507_12	euxassay_004507_13	euxassay_004507_14

euxassay_004507_15	euxassay_004507_16	euxassay_004507_17	euxassay_004507_18	euxassay_004507_19

euxassay_004507_20	euxassay_004507_21	euxassay_004507_22	euxassay_004507_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7363_wholemount_strong.wlz

7363_wholemount_moderate.wlz

7363_wholemount_weak.wlz

7363_wholemount_possible.wlz

7363_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7363_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 17 18 19 20 21 22 23

mandible

moderate

regional

moderate expression: see section 03 04 05 06 07 08 15 16 17 18 19 20 21

meckel's cartilage

moderate

regional

moderate expression: see section 07 08 09 15 16 17 18 19

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 14 15

lower jaw molar

moderate

regional

moderate expression: see section 07 08 09 16 17 18

maxilla

moderate

regional

moderate expression: see section 05 06 07 09 16 17 18 19 20

upper jaw incisor

moderate

regional

moderate expression: see section 10 11 15

upper jaw molar

moderate

regional

moderate expression: see section 07 08 09 16 17 18

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 20 21 22 23 weak expression: see section 19

clavicle

moderate

regional

moderate expression: see section 08 09 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6462
Entity Detected:	Tgfb1, transforming growth factor, beta 1 ( MGI:98725)
Sequence:	sense strand is shown >T6462 GGACCCTCTCTCCCCCGAGAGGCAGATCTCCCTCGGACCTGCTGGCAGTAGCTCCCCTATTTAAGAACAC CCACTTTTGGATCTCAGAGAGCGCTCATCTCGATTTTTACCCTGGTGGTATACTGAGACACCTTGGTGTC AGAGCCTCACCGCGACTCCTGCTGCTTTCTCCCTCAACCTCAAATTATTCAGGACTATCACCTACCTTTC CTTGGGAGACCCCACCCCACAAGCCCTGCAGGGGCGGGGCCTCCGCATCCCACCTTTGCCGAGGGTTCCC GCTCTCCGAAGTGCCGTGGGGCGCCGCCTCCCCCATGCCGCCCTCGGGGCTGCGGCTACTGCCGCTTCTG CTCCCACTCCCGTGGCTTCTAGTGCTGACGCCCGGGAGGCCAGCCGCGGGACTCTCCACCTGCAAGACCA TCGACATGGAGCTGGTGAAACGGAAGCGCATCGAAGCCATCCGTGGCCAGATCCTGTCCAAACTAAGGCT CGCCAGTCCCCCAAGCCAGGGGGAGGTACCGCCCGGCCCGCTGCCCGAGGCGGTGCTCGCTTTGTACAAC AGCACCCGCGACCGGGTGGCAGGCGAGAGCGCCGACCCAGAGCCGGAGCCCGAAGCGGACTACTATGCTA AAGAGGTCACCCGCGTGCTAATGGTGGACCGCAACAACGCCATCTATGAGAAAACCAAAGACATCTCACA CAGTATATATATGTTCTTCAATACGTCAGACATTCGGGAAGCAGTGCCCGAACCCCCATTGCTGTCCCGT GCAGAGCTGCGCTTGCAGAGATTAAAATCAAGTGTGGAGCAACATGTGGAACTCTACCAGAAATATAGCA ACAATTCCTGGCGTTACCTTGGTAACCGGCTGCTGACCCCCACTGATACGCCTGAGTGGCTGTCTTTTGA CGTCACTGGAGTTGTACGGCAGTGGCTGAACCAAGGAGACGGAATACAGGGCTTTCGATTCAGCGCTCAC TGCTCTTGTGACAGCAAAGATAACAAACTCCACGTGGAAATCAACGGGATCAGCCCCAAACGTCGGGGCG ACCTGGGCACCATCCATGACATGAACCGGCCCTTCCTGCTCCTCATGGCCACCCCCCTGGAAAGGGCCCA GCACCTGCACAGCTCACGGCACCGGAGAGCCCTGGATACCAACTATTGCTTCAGCTCCACAGAGAAGAAC TGCTGTGTGCGGCAGCTGTACATTGACTTTAGGAAGGACCTGGGTTGGAAGTGGATCCACGAGCCCAAGG GCTACCATGCCAACTTCTGTCTGGGACCCTGCCCCTATATTTGGAGCCTGGACACACAGTACAGCAAGGT CCTTGCCCTCTACAACCAACACAACCCGGGCGCTTCGGCGTCACCGTGCTGCGTGCCGCAGGCTTTGGAG CCACTGCCCATCGTCTACTACGTGGGTCGCAAGCCCAAGGTGGAGCAGTTGTCCAACATGATTGTGCGCT CCTGCAAGTGCAGCTGAAGCCCCGCCCCGCCCCGCCCCTCCCGGCAGGCCCGGCCCCGCCCCCGCCCCGC CCGCAGCGCCCGGGGCTGTATTTAAGGACACCTGCACCCCCCCTCAAGCCCACCTGGGGGGCCCATTAAA GGTGACAGAGGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3586216 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3586216 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7362 same embryo
	EMAGE:7361 same embryo
	EMAGE:7364 same embryo
	EMAGE:7360 same embryo
	EMAGE:7359 same embryo
	EurExpress:euxassay_004507 same experiment
	MGI:4828678 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source