EMAGE mouse gene expression spatial database

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EMAGE:7425

Efcab2 EF-hand calcium binding domain 2 ( MGI:1915476)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004433. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004433_01	euxassay_004433_02	euxassay_004433_03	euxassay_004433_04

euxassay_004433_05	euxassay_004433_06	euxassay_004433_07	euxassay_004433_08	euxassay_004433_09

euxassay_004433_10	euxassay_004433_11	euxassay_004433_12	euxassay_004433_13	euxassay_004433_14

euxassay_004433_15	euxassay_004433_16	euxassay_004433_17	euxassay_004433_18	euxassay_004433_19

euxassay_004433_20	euxassay_004433_21	euxassay_004433_22	euxassay_004433_23	euxassay_004433_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7425_wholemount_strong.wlz

7425_wholemount_moderate.wlz

7425_wholemount_weak.wlz

7425_wholemount_possible.wlz

7425_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7425_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon meninges

moderate

regional

moderate expression: see section 13 weak expression: see section 08 09 10 11 12 14 15 16 17 18

choroid invagination

moderate

regional

moderate expression: see section 06 07 08 09 15 16 17 18 19 weak expression: see section 10

telencephalon mantle layer

moderate

regional

moderate expression: see section 13

telencephalon meninges

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain meninges

moderate

regional

moderate expression: see section 12 13 weak expression: see section 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22

midbrain meninges

moderate

regional

moderate expression: see section 12 13 weak expression: see section 06 07 08 09 10 11 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7553
Entity Detected:	Efcab2, EF-hand calcium binding domain 2 ( MGI:1915476)
Sequence:	sense strand is shown >T7553 AGAAATAAGCAAGGCACACTACGTCCTTGACAGTCACAATCAGGCCCAGGCGGGTTCCGGAGCCTTGTTA ATAAATGACGCCCCTAGGCCCAAAGAACGCGGGCCTCGGTTCTGGTGGGGGCGGGGATCGGGGGGCCACC GTGCATCCAGGGGCTTTCTCCGCCCGCGGGGTCCCGCAGGGTTCGTGCTGGATGTCTCCCGGGCGACCGG CTCCGCACCAAGATGGCGGAGGAAAGGGACGCGGAAGGGACAGAGGCACTAATTGCAGAACTTCATAAGA AGATCAAAGACGCATTTGAAGTGTTCGACCATGAATCGAATAATACAGTAGATGTGAGAGAGATTGGGAC AATTATCAGGTCATTAGGATGCTGCCCGACTGAAGGAGAGCTGCATGATTTCATTGCAGAGATAGAGGAA GAAGAACCCACTGGCTACATTCGATTTGAAAAATTTATTCCAGTGATGACCAGAGCCCTTGTAGAAAGAA GATACAGACCAGCTGCTGAAGATATCCTTTTGCGAGCTTTCGAGGTTTTGGATCCAGCTAAACGCGGGTT TCTCACTAAGGATGAACTTGTCAAGTACATGACTGAGGAAGGTGAGCCTTTTTCCCAAGAGGAAATGGAA GAAATGTTGTCTGCTGCAATTGATCCCGAATCAAACACCATTAATTATAGGGACTACATAACAATGATGG TGGTAGATGAAAATTAAATGCTGCAAAGACTTCTTTTTTCTCCTTTTAAGACAACTGTAAACATTCAATT TCTAGTTGAAATGATAATTTGAAAATTTGTCCTAATTAATTTCACATTTTATTGTATAATTTCTACATTT AGTGCCTTATGACATAATTGTTTCAAGGTATTTTGTTTACTAGAGATGATCAGTTTAAAACATAAATTTA TTTAGTATGACCAGATTTATGGAAGGACAAGTTAATTATTTCAAAGGAATTGTACAGTATTTTAATGTTA AATGTATCGTTGCTTATTTTCCACAACTAGGCCAATGATACATTGAAACTAATTAGAAAGTGAGATTGAT CCTAACCCCCTAACATTTGCCATAGAAAGCTCTTGCAGGGCAGGATGGCCACAGACTAGATGTGAGTGGC AGCTTGGTGATGGCTGCCTTTCAGAGCAGCAGTAAACACTTCAGCAAGCAGAATATGGAGGGAGAGCGAC CAGAATAACTATTAATAATTGATTTCTTATGAATGCCAATTACAAATCTCATTTGAGAGTCTAATAACTT TGAGTAAAATTGAAAATTGCTCATCCAGCATTTTGCCATTTATGGAATAGCTAAAGGAGGTGAGATAATT GGATCCAGAGAAAGGAAGACTGAATGAAAGCTGATAATGGCAACTTCTTTTTTTGCTCTTTAGAATTCTC ACCTATTCAGAATATCTGTCATGGGAATATTGTCCTGGATACTGCGTGTATTCATCTGTTGTTACCAGAA TGTAGTATCTGATAAGGGCTGACTCCAGAAGAAAGAGATTCATTTAGCTCACAGTTATAGATGGTCAAGG TCCAGGCAGCATTGTACAGGCTGAGCTAGGGCCACCCGTGTATGCATCTCACCAAGGTACAGCTAGCCAG ACAGAAGCACAAGCAAGCAAGAGCAGAGAGGCTGGGAGGAGGGGTCAGGCCTGTTCCTTTTACAATAGTC CTCTCATAAGAATTACTTTCCAGGCTGGGGAGATGGCTCAATGGGTAAAGCATTTGCTGAGTATCATTAG ATAATGTGGTGGAAGCTGGAGATGAAAGATGGATAAACCTCTAATTAATCAAGGTCACAGTTTAGTGAGG GAGTCTTGCAAGTAAGTCAATGAGCAACCACACATTCAGGGGACTGAAGGTTGCACAAAAGAAGCAAAAG AAGCCTGGTGGGATGAGGGTGCTTTCTGGTGGGCAGACTCAAAGAGGTAACACATGACTTACAAACTCAG TAGTTCTTAAGTTTTGACAAATAGTCATTCTTTGAGCAGTTTACTTCAGAGCATGTCAGCAGCAGGACAG CAGATCAAACCCCCAGAGGTGTCCTGAAACGCAAGAATCTGGGATTGAAAGTATGGTTGATAGATATTTG GAACCTGATGAGAGATGAGAGGTTGAAATAAGAACCATATCCTAAGAGATAATGAGCTAATTGGGGTTAA GGTTTCAGTGTTTGAACGTGAACTTTCTCTGAGAATCAGAGAGAAAGGAGCTGGAGTTGTAAATAGCCTG AGAAATACTTTGAAGGGTCAAGGCCAGAGGGAAGGAGACCAGCTGGTAGGCTGCTTCGTTGGCAGTGAGA GATGGGCTAGGGAAAGTCAAGCACAAATCCTAAGTGTCTGGCTTGAACACTTGGTAAATAATAATATCAG CCATTCAGATAAGATGCCCAAGGCTTAGGCTAATAGGCTTGACTGTGGTCATCTCCAGCTTGAAGAGCTG GTAGAAGATAAAAAGATCAAGTGGATAAATAAGGGGACATCTGCTAGAGACACAGGCTTTGAAGTTGTTA GCACACGGTGTTCTGGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4240831 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4240831 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7424 same embryo
	EMAGE:7429 same embryo
	EMAGE:7426 same embryo
	EMAGE:7428 same embryo
	EMAGE:7427 same embryo
	EurExpress:euxassay_004433 same experiment
	MGI:4824479 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source