EMAGE mouse gene expression spatial database

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EMAGE:7429

Fbxo25 F-box protein 25 ( MGI:1914072)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004436. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004436_01	euxassay_004436_02	euxassay_004436_03	euxassay_004436_04

euxassay_004436_05	euxassay_004436_06	euxassay_004436_07	euxassay_004436_08	euxassay_004436_09

euxassay_004436_10	euxassay_004436_11	euxassay_004436_12	euxassay_004436_13	euxassay_004436_14

euxassay_004436_15	euxassay_004436_16	euxassay_004436_17	euxassay_004436_18	euxassay_004436_19

euxassay_004436_20	euxassay_004436_21	euxassay_004436_22	euxassay_004436_23	euxassay_004436_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7429_wholemount_strong.wlz

7429_wholemount_moderate.wlz

7429_wholemount_weak.wlz

7429_wholemount_possible.wlz

7429_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7429_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 07 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 10 16

cervical ganglion

moderate

regional

moderate expression: see section 08 09 16 17

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7555
Entity Detected:	Fbxo25, F-box protein 25 ( MGI:1914072)
Sequence:	sense strand is shown >T7555 GCTCGGTGTGGTTGGTGGCTGTCTCGTCTGGAAGGGACCCGCAGGAGCACGATGCCGTTTCTGGGTCAGG ACTGGAGGTCCCCTGGATGGAGCTGGATTAAAACCGAGGATGGGTGGAAAAGGTGTGACCCCTGTAGCCA CGAGCTCAGGAGCGAGGATAGCCAGTACACCATCAACCACAGCATTATCTTAAATAGTGGTGAAGAAGAA ATATTCAACAATGAGTGTGAATACGCAGCCAAAAAGAGGAAGAAGGAACATTTTGGAAATGACACAGCTG CTCACAGTTTTTATCGTGAAAAATGGATCTACGTCCATAAGGAAAGCACAAAGGAAAGGCATGGCTACTG CACCCTGGGAGAAGCCTTCAACCGGCTAGACTTCTCAAGTGCCATTCAGGACATCAGGAGGTTCACTTAC GTGGTCAAACTACTGCAGCTAATTGCGAAGTCCCAGTTAACCTCACTGAGTGGAGTGGCACAGAAGAACT ACTTCAACATTTTGGATAAAATCGTCCAAAAGGTTCTTGATGACCATCAGAACCCTCGCCTGATCAAAGG CCTCCTCCAGGACCTGAGCTCCACCCTTGGCATCCTGGTGAGAGGGGTGGGGAAGTCAGTGCTAGTCGGG AACATCAACATCTGGATCTGCCGCCTGGAGACCGTCCTCAGCTGGCAGCAGCAGCTGCAGAACCTCCAGG TGACAAAGCAGGTAAACACTGGCCTCACACTCAGTGACCTGCCCCTGCACATGCTGAACAACATCCTGTA CCGTTTCTCAGATGGCTGGGACATTGTCACCCTGGGCCAGGTAACACCAACCCTGTACATGCTCAGTGAA GACAGGCGGCTGTGGAAGCGACTGTGTCAGTACCACTTTGCTGAGCAGCAGTTTTGTAGACATTTGATCC TTTCAGAAAAAGGTCATATCGAGTGGAAGCTGATGTATTTCACGCTTCAGAAGTATTACCCGACCAAAGA GCAGTACGGGGACACGCTACACTTCTGCCGCCACTGCAGCATCCTCTTCTGGAAGGACTCGGGACACCCC TGCACCGCAGCTGACCCCGACAGCTGCTTCACTCCTGTGTCTCCGGAGCACTTCATTGACCTCTTCAAGT TCTGATGGTGCACTCAGCCCCGTGCCCTTGTCTACAGCTGTCCCTTGTGCTTTCTGTGTCGTGTAGTTGG TGTGAGTATTCTGTGACACAAGTGACGACAGGCTCAGTGACTGCCTGTACCTGTGCAACCCTCGCTTGCT CCTTGGAGAACCTGACAGGACGCTGAAGATGTGCGGGCAGTTGTGCACCGTCAGTGAGGGAGAGAGACCC CGACTCCATTTGTACTTCTTTCTTGACACTCTTCTCAGCTTATAAAATTTGCCGTTTTGTATATTTTTAA ATATGGATCAAAAAGAGTCTTGGATACTCAGCCCCATATTTTGTGCTGAACTGACATGAACAACCGAGAA TGTCCTTCTTGGTTGTCAAAAGACAGTTTTCCATCCTACAGTAATTGGGATGAAGAACAAAACAGTTGAT CCTTGTAAATACATTATGCAGAATATTTATTTTTCTTAAAATGTATTTTCACAACCCCAGTGGCTGTGGC CAAATGAGTCCTCATTGAACACGAGATGGAGCTAGGAATGCACGTGGGGCTGTGAACTGGATGCACTGGC CATAGCTAGCATCAATGTCCCCGTGTTTCCAGGAACCCCTGCCATGAGGCCATCATAGATCCAGACTCGG GATGTGGGCTCCAAACCAGAATAGAGCCTCTGCCAAGGAGCTGGAAACAGCCCAGTGAGGCTGAAGTTCT CTTGTAGAAATTCCTTTCCTGGGTAAAGTGATGAGCCATGTTTATCTACTGATCAGCTTCATCAAATTCG CATCTAACTGAATGGTAGTGTCTTTAAAATGTGCTGTTTTATTTTAAAAATAATGCATGTGCACTTTAGA AATTTTTGAAAATAAAGATACATGAAAGAAAGTTAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4240953 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4240953 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7425 same embryo
	EMAGE:7424 same embryo
	EMAGE:7426 same embryo
	EMAGE:7428 same embryo
	EMAGE:7427 same embryo
	EurExpress:euxassay_004436 same experiment
	MGI:4824803 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source