EMAGE mouse gene expression spatial database

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EMAGE:7427

Wwox WW domain-containing oxidoreductase ( MGI:1931237)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004450. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004450_01	euxassay_004450_02	euxassay_004450_03	euxassay_004450_04

euxassay_004450_05	euxassay_004450_06	euxassay_004450_07	euxassay_004450_08	euxassay_004450_09

euxassay_004450_10	euxassay_004450_11	euxassay_004450_12	euxassay_004450_13	euxassay_004450_14

euxassay_004450_15	euxassay_004450_16	euxassay_004450_17	euxassay_004450_18	euxassay_004450_19

euxassay_004450_20	euxassay_004450_21	euxassay_004450_22	euxassay_004450_23	euxassay_004450_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7427_wholemount_strong.wlz

7427_wholemount_moderate.wlz

7427_wholemount_weak.wlz

7427_wholemount_possible.wlz

7427_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7427_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7554
Entity Detected:	Wwox, WW domain-containing oxidoreductase ( MGI:1931237)
Sequence:	sense strand is shown >T7554 CCTCCCGGACGCGCGCGGCGCGGTTGAAGCCAGAGTGAGTTGCTGAGGAGAGCAGGCGGCTACTTCTGTC TCCAGCATGGCAGCTCTGCGCTATGCGGGCCTGGACGACACGGACAGTGAGGATGAGCTGCCTCCGGGCT GGGAGGAGAGAACCACCAAGGACGGCTGGGTGTACTACGCCAATCACACTGAGGAGAAGACCCAGTGGGA ACATCCGAAAACCGGCAAGAGGAAACGGGTCGCAGGAGATTTGCCGTATGGATGGGAACAGGAGACTGAC GAGAACGGACAAGTGTTTTTCGTTGACCACATAAATAAAAGAACTACCTACTTGGATCCAAGATTGGCAT TTACCGTGGACGATAATCCGACGAAGCCGACCACCAGACAGAGATACGACGGGAGCACCACCGCCATGGA GATCCTGCAGGGCCGGGACTTCACAGGCAAGGTGGTCCTGGTTACTGGAGCAAATTCGGGAATAGGGTTC GAGACTGCCAAGTCCTTTGCCCTCCATGGCGCGCATGTGATCCTGGCCTGTAGGAACCTGAGCAGAGCCA GCGAGGCCGTGTCCCGCATCCTGGAGGAGTGGCACAAAGCCAAGGTGGAAGCAATGACCCTGGACCTGGC CGTGCTCCGTAGCGTGCAGCATTTTGCCGAAGCATTCAAGGCCAAGAATGTGTCTCTTCACGTGCTTGTG TGTAATGCAGGGACGTTTGCTCTGCCCTGGGGCCTCACGAAGGATGGCCTGGAGACAACCTTTCAGGTGA ACCACCTGGGACATTTCTACCTTGTCCAACTCCTGCAGGATGTTCTGTGCCGCTCATCCCCCGCCCGTGT CATCGTGGTCTCTTCCGAGTCCCACAGATTTACAGATATTAATGACTCCTCCGGGAAACTTGACCTCAGC CGCCTGTCTCCACCGAGGAGCGACTACTGGGCCATGCTGGCTTATAACAGATCTAAACTCTGCAATATCC TCTTCTCCAATGAACTGCACCGTCGCCTCTCCCCGCGCGGGGTCACATCCAACGCAGTGCACCCTGGGAA CATGATGTACTCAGCCATCCATCGCAACTCATGGGTGTACAAGCTGCTGTTCACCTTGGCCAGGCCGTTC ACCAAGTCCATGCAACAGGGAGCTGCTACCACTGTCTACTGTGCAGTCGCCCCAGAGCTGGAGGGCCTCG GAGGGATGTATTTCAACAACTGCTGCCGTTGCCTGCCATCCGAGGAGGCTCAGAGTGAAGAGACAGCTCG GGCCCTGTGGGAACTCAGTGAGAGGCTCATCCAGGACCGACTGGGTAGTCCATCCAGCTAATTGGAACCC CATAATATTGGGCAAAACCCCACCCATGCTCTGACCCTCATCACAGTCAGCCCCATCATCCTGGGTGCCC TGAACTCCAGTACAGGGCAATGAGAGTAGATAAGAACAATCAATGGGAGAAGGTTATAACTAGTGGGAAG CAGGGAATTTCAAGAGATGATGTCCCTTTTCTAGGACTGGGTTAGTCGTGGGACTGCTTGCTTTTCTGGT GTTGGCCCGCTTGAAAATGGAATCATGGTTAGCATGTAGGTTCCCTTGCTCATTGTAAAAGCACACGTGA TTTTCTATCAACCTTAGAATAACCTGATATCCCCTCGACCCACCCAGCTGCCACCACTGCCAACTTTGCC ACCCAAGGTTGGCTTTCTCCTGTTAATGGAAGAAAAAAAAAACCACACACATTGTTCACTTCTTCAGGCT GAGTTCAATCCTGGAGGCCATGTTTCACACTTCCCTACCAAGTCTGAGCAGGCGTGGCAACTTGAGAGAA AAACTCAGTACCTAGTCCCAAGAGAACCAATGCGACGGTGCCATCAGAGAGGAGCAGTGTCGGTGTGGTG TGCAGAGCTGGTGGTTCCCAGAAGCACCATTCCCTGACCTCCTTGCCAAAGTTAACACAAGCAATTCTTT AGAGATTATAACGAGTGCATTTTACATTTTGCAAACCATTCCCTAGGCAGGCAAGGAAGGAGGCACGGTG TCCTTAAGATACATGGGATATGCTGGAGGTGGGAAGCAAGGAAGTAGTTCCCTCCAGTCTTCACTCACAG TTCCAAGAACATCTTCTTTAGCGACTGTCCCAGCCTCTAAGTCCTCTGGAGTTCACTTCCCCTGGCTCCC CCATCCTATGCTTAATAAAAGAACATGCTTAACAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4241066 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4241066 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7425 same embryo
	EMAGE:7424 same embryo
	EMAGE:7429 same embryo
	EMAGE:7426 same embryo
	EMAGE:7428 same embryo
	EurExpress:euxassay_004450 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source