EMAGE mouse gene expression spatial database

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EMAGE:7428

Tbcc tubulin-specific chaperone C ( MGI:1919976)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004448. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004448_01	euxassay_004448_02	euxassay_004448_03	euxassay_004448_04

euxassay_004448_05	euxassay_004448_06	euxassay_004448_07	euxassay_004448_08	euxassay_004448_09

euxassay_004448_10	euxassay_004448_11	euxassay_004448_12	euxassay_004448_13	euxassay_004448_14

euxassay_004448_15	euxassay_004448_16	euxassay_004448_17	euxassay_004448_18	euxassay_004448_19

euxassay_004448_20	euxassay_004448_21	euxassay_004448_22	euxassay_004448_23	euxassay_004448_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7428_wholemount_strong.wlz

7428_wholemount_moderate.wlz

7428_wholemount_weak.wlz

7428_wholemount_possible.wlz

7428_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7428_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 16 17 18 19 20 21 22 23 24 weak expression: see section 11 15

humerus

moderate

regional

moderate expression: see section 01 02 23 24

hand

weak

regional

weak expression: see section 01 02

foot

moderate

regional

moderate expression: see section 03 19 20 21 weak expression: see section 04

fibula

moderate

regional

moderate expression: see section 01 02 23 24

tibia

moderate

regional

moderate expression: see section 01 02 23 24

femur

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23

meckel's cartilage

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

axial skeleton

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

pectoral girdle and thoracic body wall skeleton

moderate

regional

moderate expression: see section 03 04 21 22 23

pelvic girdle skeleton

moderate

regional

moderate expression: see section 08 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7556
Entity Detected:	Tbcc, tubulin-specific chaperone C ( MGI:1919976)
Sequence:	sense strand is shown >T7556 GGTCAAGATGGAGGGTGTGGACTGCTCCATGGCTCTGGCCGACGCTGCCGCTGGCTCCCCGCGGGACCTG AGCCTGGTGCCCGAGCGACTTCAGAGGCGCGAACAGGAGAGGCAGATAGAGGTGGAGAGACGCAAGCAGA AGCGGCAGGACCAGGAGGTGGAGGAGGAGAAGAGCGGCTTCTTCGCCGCCGCCTTCGCCCGGGAGCGCGC AGCGGTCGAGGAGCTGCTGCGGGGCGAGGCGTCGGCGGAGCGGCTGGAAGAGGCGGCCAACCGGCTCCAG GGCCTGCGGAAGCTCCTGAACGACTCGGTGCTCTTCCTCGCCGCCTACGACCTGCGGCAGGGCCAGGCGG CGTTGGCGCAGCTGCAGGCTGTCCTAACGGAACGTCGCCAGGAGTTGCAGCCCAAGAAGCGCTTCGCCTT CAAGGCACGGAAGAAGGATGCTGCGGGGACCGCCCAGGTAGACGCCGCACCCGTCACCTCGGCGGCCCCT TCTCCACCGGTGACCAAGGAGGAGGAGGGAGCTCCCGGGGCCAGCTGGGCCTGTGGCTTCTCCAACCTGG AGTCCCAAGACCTGGAGAAGAGAGCTGAAGAGCTGCACCAGCGCGACGTCCTTTTGAGCGACCTGACCAA CTGCACTGTCAAACTGTGTGGCAATCCCAACACCTTGCGGCTTGCGAAGGCCCGCGGCTGCAAGGTGCTC TGCGGCCCAGTGACCACCTCTGTGTTTCTGGAGGATTGTCGAGATTGCGTCCTGGCTGTGGCTTGCCAGC AGCTCCGTGTGCACACTACCAAAGACACGCGCGTCTTCTTGCAAGTGACCAGCAGGGCTATCGTGGAGGA CTGCAGCGGCATCCAGTTTGCCCCTTACACCTGGAGCTACCCGGGGATCGACAAGGACTTCCAGGACTCC GGTTTAGATAGGAGCAAAAATAACTGGGACCAGGTTGATGATTTCAACTGGCTGGCCCGAAACGTGGCCT CCCCAAACTGGAGTATTCTTCCTGAAGAGGAGAGAGACATCCAGTGGGATTGAGCACTGGCCACACTCAG TGTGATGTCCCATTCGGTTTACCTGATATTGTCTTATGTTTGCGGTGATAGGGGTTAGAAATTGTTGAAA CTCCCCTCCTGTGAGTCCTATTAAATTCATAGCAGTGTAACAGACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4240883 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4240883 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7425 same embryo
	EMAGE:7424 same embryo
	EMAGE:7429 same embryo
	EMAGE:7426 same embryo
	EMAGE:7427 same embryo
	EurExpress:euxassay_004448 same experiment
	MGI:4828611 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source