EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7568

Casp9 caspase 9 ( MGI:1277950)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004688. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004688_01	euxassay_004688_02	euxassay_004688_03	euxassay_004688_04

euxassay_004688_05	euxassay_004688_06	euxassay_004688_07	euxassay_004688_08	euxassay_004688_09

euxassay_004688_10	euxassay_004688_11	euxassay_004688_12	euxassay_004688_13	euxassay_004688_14

euxassay_004688_15	euxassay_004688_16	euxassay_004688_17	euxassay_004688_18	euxassay_004688_19

euxassay_004688_20	euxassay_004688_21	euxassay_004688_22	euxassay_004688_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7568_wholemount_strong.wlz

7568_wholemount_moderate.wlz

7568_wholemount_weak.wlz

7568_wholemount_possible.wlz

7568_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7568_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8653
Entity Detected:	Casp9, caspase 9 ( MGI:1277950)
Sequence:	sense strand is shown >T8653 TGGGGGCTGGTGGTGGGGTGAGCGGCCGTGGGAGCTGTTTTGATTGCGGAGGTGGAGCCCAGTGAGCGCG AGTCACAGGCCGCGGGGCGGGGCGGGGCGGGGCGGAGCGGAGCCCAGTGGACCGGGTGACCGCTGGCCGG AGCGGGATCCCTGGAGGCGCTATGTGGGGGCGGGGCGTGGGCGCCGGGGCGGAACCCGAGGGCCGGAAGC GGACAGAACCCGGGGTCCCGCAATCGGCTCGCCATGGACGAGGCGGACCGGCAGCTCCTGCGGCGATGCA GGGTGCGCCTAGTGAGCGAGCTGCAAGTCGCGGAGCTCTGGGACGCTCTGCTGAGTCGAGAGCTCTTCAC GCGCGACATGATCGAGGATATTCAGCAGGCAGGATCTGGGTCTCGGCGGGATCAGGCCAGGCAGCTGGTC ACAGACCTTGAGACCCGAGGGAGGCAGGCCCTTCCTCTCTTCATCTCCTGCTTAGAGGACACAGGCCAAG GCACCCTGGCTTCACTCTTGCAAAGCGGTCGGCAAGCAGCCAAGCAGGATCCAGAGGCTGTTAAACCCCT AGACCACCTGGTGCCTGTGGTCCTGGGACCAATGGGACTCACAGCAAAGGAGCAGAGAGTAGTGAAGCTG GACCCGTCACAGCCTGCCGTGGGAAACCTCACCCCAGTGGTGCTGGGGCCAGAAGAGCTCTGGCCTGCTC GGCTCAAGCCAGAGGTTCTCAGACCAGAAACACCCAGGCCCGTGGACATTGGTTCTGGCGGAGCTCATGA TGTCTGTGTTCCAGGGAAGATCAGGGGACATGCAGATATGGCATACACCCTGGATTCGGATCCCTGTGGC CACTGCCTCATCATCAACAATGTGAACTTCTGCCCTTCCTCGGGGCTCGGCACACGCACGGGCTCCAACT TGGACCGTGACAAACTCGAGCACCGATTCCGCTGGCTGCGCTTCATGGTGGAGGTGAAGAACGACCTGAC TGCCAAGAAAATGGTCACGGCTTTGATGGAGATGGCACACCGGAACCACCGTGCCCTGGACTGCTTTGTG GTGGTCATCCTCTCTCATGGCTGCCAGGCCAGCCACCTCCAGTTCCCGGGTGCTGTCTATGGGACAGATG GATGCTCCGTGTCCATTGAGAAAATTGTGAATATCTTCAACGGGAGCGGCTGCCCCAGCCTGGGAGGGAA GCCCAAGCTCTTCTTCATCCAGGCCTGCGGTGGTGAGCAGAAAGACCATGGCTTTGAGGTGGCCTGCACT TCCTCTCAAGGCAGGACCTTGGACAGTGACTCTGAGCCAGATGCTGTCCCCTATCAGGAAGGCCCAAGGC CCTTGGACCAGCTGGATGCTGTGTCAAGTTTGCCTACCCCCAGTGACATCCTTGTGTCCTACTCCACCTT CCCAGGTTTTGTCTCCTGGAGGGACAAGAAAAGTGGCTCCTGGTACATCGAGACCTTGGATGGCATTCTG GAGCAGTGGGCTCGCTCTGAAGACCTGCAGTCCCTCCTTCTCAGGGTTGCCAATGCTGTTTCTGCGAAAG GGACTTACAAGCAGATTCCTGGCTGTTTTAACTTCCTCCGGAAAAAGCTGTTTTTTAAAACTTCATGAGG CTCGGGGCTGCTCTCTGCCTCATTATTCTCTCCGAAGCTCTCATGGCTTGGAGCTGGCCAAGGCCATAAC TGTCCTGCTAAGATAGGATTTTGAACTGGGGCAGGCTGCTCTTTCCCTTTGGCGATGCAAACATGCTCCT AGCAGCTTTCAGGTTGTAGGGCAATGTGGAGCAGTGGTCGGAAGAAGACCGGAGTGCAATGGATGCTGCC TGGCCCCTTCATCTGTGGTTAGGGATTGTGCCCTGGGACTCCCAGGCCTCTCAAGAACAGGGCTTCTCTG CCACTGTACTACTGACATGTGCGGTTGTATAATCCTGTCATGGGACTGCCTCCTGTATTGTAGAGGGTTT TAAGCTGTCTCTGTCCTCTACCTGCCAGCTGCCAGGAGCACCCCTCCTGAGATCTGCCAACCAAAAATGT CTTCAGACATTGTCACATGTGCCCTGGGGGACATTATAGCACTCCTCTCTGCTCCCCCACCCGGGGGGGG GTCATCAACCACAGGGGTCTATGCATCTCTCCTTTCCAGACTCAAAGTCCAGCTGGCCCAGTGTGCATAC CTGGATCTGGGCTGCATCTCTTCCAAGTTACCAGAAGGGCTAATTCTTCCTTGAGAACATTATTTATTCT TCAACAATAAATCTTCGGCAATAGGACTGTATTTAGCCTGTATTGTCCCTACCCCAACTCTGTCCTTCTT ACAAAGGAGACATGATTTTTCCTGCGTTTTTCTCTGTGTCTTCCAAGGTCCCTGCCCGAGCCCAGCAGGT GACCGAGATAGGAGTCAGGAGTCCCCACAGATCAAGTCTCCTGTCTGAAGGATCTTGGTTACCCTAGTGG GGACACCAGGACAGAGCTCTGCTGTTTGGGGTCTCCCAATCTCCCATTGTCTTCCTTGCTAAGTGAGCCT GGTCCCCAGGTGGCAGTGACCAGGCAGTACTGAAGTGCTGGGACTTCCTTGAAAATGTGCCCTCCCCTGT CTTTAAAGCGATTCTGCCTTTCACTTATTTTTCTTTCTTTCTGGTTTCCTCTACTCTTTGCTTTGTGAAA GTCCACTTGAGTTTAGATGGTAGATGTGCCAAGCCTTGTTTGTTTATTTTTGAGCTAGGGTCTTACTTTG TAGCCCAGGCTGACCACAAAATCTCCATACCACTGCCTCCTGGGTACCAGGATTATAGCTTGTCCCACTG CCCCAATCTCCTCCTGAAAGAGCATTGCTTTCAGGGCCCCGAGGTTAGGGTATCGGGGTAAGAAGACAGA CTCATTCAAGGATGTGATTCTTAAATTACTTTATTACTATTTCTTTTCTATGGCATTTCCAGGGGGACAG TGGCAGTAATGTCTTCAGTTAGTTCCTTGCCATCACCTGCAGGACACACAGGAGAGAGAGGAGGTTAGCT GGAACACTGGGCATTGAGTTCAGTGACTCTCAGGGCCAAGAGGGGTCAAGAAGGGTCTTAGCCCGGAATC CTGCTTGGGTATCAGGCCAGGAAGGCCCGGAATCACCAATCATTACATGCCAAATGCACCGATGGGTATG TTTTGGTTTTTTTTTTTTCATGTTGTCTGTTTCTTTGTTGTTGTTGTTGTTGTTGTTTTCCCTGAGACTG GGTCTCTCTGTGTAGCCCTGGCTGTCCCAGAACTCACCATGAAGACAAGACTGGCCTTGAACTCAGAGAT CCGCCTGCCCTTGCCTCTGAGTAGTGGTCTTAAAAGTGTGGACCAGTGCACAGGCCCCTCACAGTTCAGA ATGGCAGGTGCCAGCTGAGTTCATTAAACAAGGAAAATGTGTTGATGGCTTATGGTGAGCAGACCATCTA CACATGCAGTTGTGGGCGTTTCTTTGTTGTTTGTTTTGAGGCTGGGACTCACTATGTAGCCTAGGCTGGC CTTATTACCACCATACTTGGCTTCATTTGGTTGGTTGGTTTTGTTTGAGACACTGTCTCATGTAGCCCAG GCTGCCCTTAAAACAATCCTCCTGCCTCTTATTCCCAAGTTCTGGCCATTATGCATCTTTACCACCATCT CTGCCAATACAGGATCCTTTGTTTTGTTTTGTTTTGTTTTGTTTTGCAGTTTTGGCGTCTATGCACACAA GGCCCAGGTGAGCACAGAGCATACAATTGACAGCGATTGCCTCTGTGGATTGGGCGCAAAGGTTAAAGCC AGGGGAATCTTCATTTCTTACGTGATACACTCTTGTGTTCCTTTTATAAGCAATATTTTAAATAAAGCAA TGTATTCCCTTCTGAACCAAAAATAAAGCTGTGATTGTTGAAGGCTAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5721188 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5721188 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7570 same embryo
	EMAGE:7571 same embryo
	EMAGE:7567 same embryo
	EMAGE:7569 same embryo
	EMAGE:7566 same embryo
	EurExpress:euxassay_004688 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source