EMAGE mouse gene expression spatial database

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EMAGE:7569

Tmem209 transmembrane protein 209 ( MGI:1919899)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004682. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004682_01	euxassay_004682_02	euxassay_004682_03	euxassay_004682_04

euxassay_004682_05	euxassay_004682_06	euxassay_004682_07	euxassay_004682_08	euxassay_004682_09

euxassay_004682_10	euxassay_004682_11	euxassay_004682_12	euxassay_004682_13	euxassay_004682_14

euxassay_004682_15	euxassay_004682_16	euxassay_004682_17	euxassay_004682_18	euxassay_004682_19

euxassay_004682_20	euxassay_004682_21	euxassay_004682_22	euxassay_004682_23	euxassay_004682_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7569_wholemount_strong.wlz

7569_wholemount_moderate.wlz

7569_wholemount_weak.wlz

7569_wholemount_possible.wlz

7569_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7569_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

midbrain ventricular layer

moderate

regional

moderate expression: see section 12 15 16 weak expression: see section 08 09 10 11 14 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8662
Entity Detected:	Tmem209, transmembrane protein 209 ( MGI:1919899)
Sequence:	sense strand is shown >T8662 CGGCTTCCTGCTGAAGAACATGATGCAAGGAGACGTAAGCCCCAATCCTTCCCTTATTGACAGAACCATC AAGATGAGAAAAGAAACAGAAACTAGGAAAGTGGTTTTAGCTTGGGGACTCCTAAATGTATCCATGGCTG GAATGATATATACTGAAATGACTGGAAAGTTAATCAGTACATATTATAATGTGACATACTGGCCCCTCTG GTATATCGAACTCGCACTTGCATCGCTCTTCAGTCTTAATGCCTTGTTTGATTTTTGGAGATACTTCAAA TATACCGTAGCACCAACAAGTCTGGTAGTTAGCCCCGGACAGCAAGCACTGTTAGGGTTGAAACAGGCTG TTGTGCAGACTACACCTCCCCGTGATTTGGCAGCAACCCAAATCTCTCCATCTCCACCTTCCCCTTCGAT TCAAGGTCAAAGTGTGTTAAGTTATAGCCCATCTCGTTCACCCAGTACCAGTCCCAAGTTTGCCACCAGC TGTATGACTGGGTATAGCCCTCAACTGCAAGGCCTGTCGTCAGGAGGCCTTGGCTCTTACAGCCCTGGTG TGACTTACTCGCCTGTCAGTGGCTACAATAAGTTGGCCAGCTTTAGTCTGTCTCCTTCTTCTCCATACCC TACCACTGTGGGCCCAGTGGAGAGCAGTGGATTGAGAGCTCGCTACCGTTCTCCACCCACGGTCTACAAC TCACCCACTGACAAAGAGGACTACATGACAGACCTGAGAACTTTGGACACGTTCCTTAGAAGTGAGGAAG AGAAACAGCATAGAGTGAAGCTGGGGAGCCCAGATTCCACCTCTCCTTCTACCAGCCCTACCTTCTGGAA TTACAGTCGTTCTGTGGGTGATTATGCACAAACCTTAAAGAAGTTTCAGTATCAGCTTGCCTGTAGATCA CAGGCCCCATGTGCTAACAAAGATGAAGCCGATCTCATTTCTAAGCAAGCTGCAGAAGAGGTCTGGGCAA GAGTGACTATGAATAGACAGCTTCTCGATCACATGGATTCATGGACAGCCAAGTTTAGAAATTGGATCAG TGAGACAATATTGGTGCCGCTTGTGCAAGAGATTGAGTCTGTCAGCACCCAGATGAGAAGGATGGGCTGT CCAGAGCTACAAATAGGAGAGGCGAGTGTTACCAGCTTGAAGCAAGCTGCCCTGGTCAGAGCTCCTCTCA TTCCAACTCTGAATGCAATTGTGCAGTATTTAGACCTCACACCAAATCAGGAGTACTTGTTTGAAAGAAT CAAAGAACTTTCTCAGGGGGGCTGTATGAGTTCATTCAGATGGAACAGAGGTGGAGACTTCAAAGGACGC AAATGGGATACAGACCTGCCCACTGATTCTGCTATCATCATGCATGTGTTTTGCACCTACCTTGATTCCA GATTACCTCCACACCCTAAGTACCCTGATGGAAAAACATTCACTTCTCAGCACTTCGTTCAGACGCCAAA TAAACCAGATGTTACGAATGAAAATGTGTTTTGTATTTATCAGAGTGCTGTCAACCCTCCACATTATGAG CTAATCTATCAGCGGCATGTCTACAATCTTCCAAAGGGCAGGAATAATATGTTTCATACGTTGCTGATGT TCCTTTATATCATAAAGACCAAAGAGTCAGGAATGCTTGGGAGAGTTAACCTTGGGCTCTCTGGCGTGAA CATTTTGTGGATCTTTGGAGAGTAGTTCCTCATTTAATTCCAAACATTTGGACTTTGCTTTTAACTGGTC TGGAAGAAGTTGGAGCTGAGCCCCTCCGAGTGCGCACCTCTTCTGACATAAGATGCATGTTTTGCAGACT TGTTAAATTTAACAGGAGACAAGCTGTGGGAGCTTCTTGTGAGCATAGCAAAGCCTGCACCTTCTAGTCC ACAGAAAAGCATTACACTGGATGGCTCTGAGCGAGCTAGTGCCGCTCCCTTCATTTGGTGAGGGCAAGGA CGCCTCTCCATTTTAACCCCAGTGAGAACCTGCACCTCATCCTGTACCATCAGAGACTGCTTTCATTGTT GGATAGTAGTGTAAGTTCTCAGATGAACCTTGCTAGTTCTCTTTGTTGTATCTTTATATTTCCCCAGTGT TCTACAAGTCAGCCTAAAGCTGAAATGAAATTAATTTCTAAGTGATGTAGTAATTAATCCTGCTGAGCCA TCTCCCCAACCCCCTATTGTTTTTTTTAATATTAAAAGAGATTTGATAATATTGGCATCTTGAAAGGTCA ATGCTTGCATTAAAATTAGCTGATAAAATTAAACTTTTGGGTTTTAGTTAAATGTCTAAGGACACGAAAA AAACGGTTCACTGCTTTTATTAGCACTGCGTGTGCGCGAGGGCCTGTGTGCTGGCGCCTGTGTATCATCA GCAGGCACTTTATCACTCCTTTAAGATCCCAATGATAGCCTTCCTGCTTCTAGTTTACAGGTAGAACAGA GTATCAGCTCTCCATCTATTCTTCTATATGTGTACATAGATAGGGACCTATACATATCTATATGTATATG AAGTAATTAGTAGTCACATTTTTTTGGTGGGGGTTATTTTCGAGACAGAGTTTCTCTGTGTAGTCCTGGC TGTCCTAGAACTCACTCTGTAGACCAGACTGGCCTCAAACTCACAGAGATCTGCCTGCCTCGGCTTCCTG AGTGCTGGCACCAAAGGTGTGCACCACCAACGCCCGGCTAGTAGTCACATTTGTAATGAACATGTCTTTA TAAAGATTGCTTCCATCACCTGAATGTGAACAGATGGGATGTGCCGTCCATTTTTGTATGGTTTGAGGGT TTTTTTCTTCAAAAGGTCAGAAGGTTAACCTTAAGTTTAAAAGCTAATGTCCACAAGGAAGGAACTGTCA CTTAACTAACTGCTCTTGGTTACCCTAAAAGAAAATATCATTTAACCATGCTAAACTTAAGGACACGTTA TAATTTAAACCTGTAATTTTTGAGAGAGATTCGGGGTGCCATGGAATCAGTTGCTGTGCTATTTAAGTAT CCAGCTGCACAGACTGCACTAAAAACACCGGAGAATCTTTAACAGCACATAAGACACCACCAACCCTGTC TTTTATTATAACTCTGAAAGAATATAGTATAGTAGTAAATAGATGTAAAAACACTTGACTATGTAAAAAG AAAAGTTGTTTTGCCTGCATACTTAGTATAGGTTGCTGTAGAGTTCCTATAACAATGTCTACAAGTGTTT GAGCTAGCTGTACTTACTATTATCTTGTATTTAAACAGAATTGGCACAGACATGGTGGGAAATTGCCAGG AACCTCAGCTGCTCATGAGGTTGAAGTGGGAGGATCATTAGTTCAAGGCCAAACTAGGGTACCTTGTGAT AACCTTTCATAAATATAAGACATTAATAAAATAATTATAGTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6400080 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6400080 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7570 same embryo
	EMAGE:7571 same embryo
	EMAGE:7567 same embryo
	EMAGE:7568 same embryo
	EMAGE:7566 same embryo
	EurExpress:euxassay_004682 same experiment
	MGI:4828794 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source