EMAGE mouse gene expression spatial database

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EMAGE:7571

Fbxo38 F-box protein 38 ( MGI:2444639)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004672. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004672_01	euxassay_004672_02	euxassay_004672_03	euxassay_004672_04

euxassay_004672_05	euxassay_004672_06	euxassay_004672_07	euxassay_004672_08	euxassay_004672_09

euxassay_004672_10	euxassay_004672_11	euxassay_004672_12	euxassay_004672_13	euxassay_004672_14

euxassay_004672_15	euxassay_004672_16	euxassay_004672_17	euxassay_004672_18	euxassay_004672_19

euxassay_004672_20	euxassay_004672_21	euxassay_004672_22	euxassay_004672_23	euxassay_004672_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7571_wholemount_strong.wlz

7571_wholemount_moderate.wlz

7571_wholemount_weak.wlz

7571_wholemount_possible.wlz

7571_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7571_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8651
Entity Detected:	Fbxo38, F-box protein 38 ( MGI:2444639)
Sequence:	sense strand is shown >T8651 ATTCTCGGTCCCTCTCCACCACAAAAACAGACGGAGGGCCGGCGTAAGTGAGCCGCGGCCTGGGAGGCTG TCGCGACGGTGGAGGTATTTTGAACTAACTTTGACACTGAGGGAGCACAGCCATGGGACCACGAAAGAAA AGTGCTAAAGTGTGTGTGATGGACAGTGAGGTCGCTGAGGAAATGACAGCCGATGAAGAGAAGGACTACA TGAACCAGCTGTCACATGAAGTGCTCTGTCATATCTTCAGGTACCTGCCACTACAGGACATCATGTGCAT GGAATGCCTCTCTCGGAAGCTAAAGGAGGCAGTCACTCTGTATCTGAGAGTTGTCAGAGTGGTGGATCTC TGTGCAGGGCGCTGGTGGGAATATATGCCAAGTGGCTTCACAGATTCCAGTTTCCTAACCCTTTTGAAGA AGATGCCAGATGTTGAACAGCTGTATGGCCTTCACCCTAGATACCTCGAGAGGCGAAGAGTGAGGGGCCA GGAGGCTTTCAGCATCCCAGGAGTCTTGGAAGCTTTGCAAGCGTGCCCAAACTTGGTGGGTGTAGAAACA TCTCATTTGGAGTTGGTAGAATCCATTTGGACATATATGCCGCATGTTCATATTTTGGGGAAATTTCGTA ATCGTAACGGAGCATTTCCGATTCCTCCTGAAAATAAACTGAAAATCCCCATAGGAGCCAAAATCCAAAC TTTGCATTTAGTTGGTGTCAATGTTCCTGAAATCCCTTGTATCCCTATGCTAAGGCACCTGTACATGAAG TGGGTGAGACTCACTAAACCACAGCCGTTTAAAGATTTCCTTTGCATCAGCTTGCGAACTTTTGTCATGA GGAACTGTGCAGGACCAACAAACTCCTTGAAATACGTGCCCTTGGTAACAGGTCTGGCATCTGCCCGTAA CCTGGAACACTTGGAAATGGTTCGAGTCCCTTTCCTGGGAGGTCTCATCCAACACGTGGTTGAAGACAGC TGGAGATCAGGTGGTTTTAGGAACTTGCACACTATTGTCTTAGGAGCTTGCAAGAATGCCCTGGAAGTAG ACCTTGGCTACCTCATCATCACTGCTGCGCGAAGGTTACATGAAGTTCGCATCCAGCCTTCTTTAACCAA AGATGGTGTCTTTTCTGCCCTAAAGATGGCAGAATTGGAGTTTCCCCAGTTTGAAACCCTTCATCTGGGA TATGTAGATGAGTTCTTGCTACAGAGCAGAATGGCGAATGCAGATTTGGTGAAGTATGGCTTGGCTGATG TGGTAGAAAATCCTGGTATCATTACTGATATAGGGATGAAGGCAGTCAATGAAGTTTTTTCCTGTATCAA ATATCTGGCAATTTATAACTGTCCTCATCTACACAACCCATACAATTGGATCTCAGACCACTCGAGGTGG ATGCGACTGGTTGATATCAACCTCGTGCGGTGCCATGCTTTGAAGCTGGATTCTTTTGGCCAGTTTGTTG AATTATTGCCCAGCCTGGAATTTATTTCTCTGGACCAGATGTTCCGTGAGCCACCCAAGGGCTGTGCTCG AGTTGGTCTGAGTGCAGGAACTGGAATTGGGGTTTCCTCAGCGCTTGTCAGCAACCAGAATTCCAACAAT GACAATGACAATAATGCTCCAAATAACAACGCCAACCTCCATGATAACAATCACCATCACCCAGATGATT CGGATGACGACAATGACTTTCGTCCAGACCTACAAGCAGGAGAGGCACAATTTGCAGCCGATGCGTTGAA TGAGATGGAAGACATGGTCCAGGAAGATGGAGAACTGGTAGCCGAGAGTGGAAATGGTATGCCAGCTCAC AATCGGGAAGTTCTTCCTGTGGATGCTGATGAGGAACAAGCAGGACCTAGTGGTCTACAACGCGTGGTAA AACCTACACCAATTGCTGATCACGATTCAGAGAGCGATGATGAAGAAGATAGTTTAGAACTCCAAGAAGT CTGGGCTCCTAAGAATGGCACCCGGCGTTACTCAGAGCGGGAAGAAAAGACTGGAGACTCAGGACAGTCC AGGGAAACGGCAGTAAGTGGGAAAGGCAAGACTCCACTTCGAAAGAGGTGCAACAACTCCCACCAGACGG GCCAGGCGAAGCCGTTTCCCCTGGAGGAGAGCAGCTGTGAGAAAGGCTGTCAGGTGACCAGTGAGCAGAT CAAAGCCGATATGAAAGCAGCTAGGGATGTTTCTGAAAAGAAAAAATCCAAGGATGTCTATCCCAGCTGC AGCAGCAGCAGCAGCAGCACAGCCGCCAGCACAGCGGGAAACGCCAGCTCACCCAGCACTGCTTCCCAGA GCCCGGACTTTGCAAGGACGGTGACCAGCAGCGGCTCTTCCGAGCCGAGCCCTCCAGAAGTAGATGTATC CAGGCAGTGTGTCTGCTCCCCTGGTGGGTCAGAGGACTCTGAGGCCATGGAGGAGGGAGATGCAGAGAGC TCTGTCTGCCCCAGATGCTGCTGTCTCAGGCCCCAGGAGTCTCAAAGGAGAACTGGCAGGTGTTCTGATG AGGAGCGTCCTTCAACCAGCCGAGCCTGTGTTGTGAATGGCGCGGATGGTACGAGATCCGCCTTTTCCTT TAGGACTCTGCCACAAGGGGGGTCTTCAGGCCCAGCACATGATGAGAGGACTAATGGGAGTGGCTGTGGG GCTACAGGTGAGGACAGGAGGGGGAGCTCCCAGCCTGAGAGTTGTGACGTGCAGTCTAATGAAGACTATC CTCGGAGGCCCCTAACCAGGGCCAGGAGCAGACTGTCCCATGTACCGCTGATATCTGAGTCAGAAGTTGC CAAAACAAAGCCATGCCATGCCATGAAACGAAAGCGAACTGCAGATAAGTCTACCAGCACCAGTGACCCT GTGATTGAAGATGACCACGTACAGGTTCTTGTATTAAAATCCAAAAATCTTGTTGGAGTCACAATGACCA ATTGTGGAATCACAGATCTAGTACTGAAAGACTGCCCCAAGATGATGTTCATCCATGCTACCAGGTGCAG GGTACTGAAGCATTTAAAGGTAGAAAATGCACCAATTGTAAACCGATTTGACTATGCACAGTGCAAGAAA CTAAATATGGACCAGGTACTAGACCAAATACTGAGGATGCCTCCGGAGAGGAACCGGATCATCTACCTGC GCCCAATGCAGCAGGTAGACACACTAACTCTGGAGCAGAAGCTCTTCAGCGGGCCCTACCCCTATCACAT CTGCATCATCCACGAGTTCAGCAATCCTCCCAACGTGCGGAACAAGGTGCGCATCCGCAATTGGATGGAC ACGATAGCCAACATCAATCAAGAGCTCATTAAATACGAATTCTTCCTGGAAGCCACTCGGACTGAAGAGG ATTTAAAGAAGTACCCTAAGTATCCCTGGGGGAGAGAAATCTACACTTTAGAGGGTGTTGTAGATGGAGC GCCGTATTCCATGATTTCCGACTTCCCTTGGCTGAGGTCACTGCGGACCGCAGAGCCCAACAGCTTTGCC AGATATGATTTTGAAGATGATGAAGAGAGCACCATCTATGCTCCTCGAAGAAAAGGGCAGTTGTCTGCAG ACATTTGTATGGAGACAATAGGAGAGGAGATCTCAGAGATGCGCCAGATGAAGAGGGGCATATTTCAGCG AGTAGTAGCGATTTTTATCCACTACTGTGATGTCAATGGAGAGCCAGTTGAAGACGACTACATTTAAGTA GACTTTTCCTGGCTCTTCTGGCCCAGAGCAGCCAAGAGTCTCTACACAGGAACCTTGGGCTTGGAATTGG GAGGCCCTGGCTCAGTTTGTTATATAGGAAATATATAAGGAACATGGAAATTGTATACAAAGATTTATAT ATAGAAGAAATATACAAAGATACTTCCAAATGTAACATCTTTATATCCTATGTTTATACATCTTAATTTA AAGACGTCATTTGAAGGCAGGCAAATCGTTTAGCAGTGGTCGTGTTCTTTTCCTGACTTAGTTCTCAGTG TCAGGCGACTGAGCCTATTGCCTCTGCTCCTCACCACGTCCTTCAGTCTGTGCCTTTTTCTTCTCCAGCA GAGCTGTTTGAGTCACCCTCCTAAAGAGTGTTGTATCTTGTGTGTTTTTGTTACCATGATGCCAACTAAA CCCTTAGTCAGAGAAGATTATGTTTCTGCACTAAGATGTGGCTCTTCTTTTTTGTGTGTTTATATTTGGC ATATTCATTTACATGAAAAGATTGACGAACTTAAATAAATTTGAAGGGCAAATAAAAAAAAAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5721407 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5721407 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7570 same embryo
	EMAGE:7567 same embryo
	EMAGE:7569 same embryo
	EMAGE:7568 same embryo
	EMAGE:7566 same embryo
	EurExpress:euxassay_004672 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source