EMAGE mouse gene expression spatial database

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EMAGE:7644

Mcart1 mitochondrial carrier triple repeat 1 ( MGI:2684984)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005285. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005285_01	euxassay_005285_02	euxassay_005285_03	euxassay_005285_04

euxassay_005285_05	euxassay_005285_06	euxassay_005285_07	euxassay_005285_08	euxassay_005285_09

euxassay_005285_10	euxassay_005285_11	euxassay_005285_12	euxassay_005285_13	euxassay_005285_14

euxassay_005285_15	euxassay_005285_16	euxassay_005285_17	euxassay_005285_18	euxassay_005285_19

euxassay_005285_20	euxassay_005285_21	euxassay_005285_22	euxassay_005285_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7644_wholemount_strong.wlz

7644_wholemount_moderate.wlz

7644_wholemount_weak.wlz

7644_wholemount_possible.wlz

7644_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7644_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8932
Entity Detected:	Mcart1, mitochondrial carrier triple repeat 1 ( MGI:2684984)
Sequence:	sense strand is shown >T8932 AGAAGGCTAACAATGGACAACACTGGGAAAGTTCCCAGAAAGCTGTCATCCTCAGTGCCAGGCTCAGATG TCACAGGTTAAAGAAACTCTTCCCCAGAGAGGAAAGGCCGGAAGCACCATGATGGACTCCGAAGCACATG AGAAGAGGCCCCCAATGTTGACCTCTTCGAACCAGGACTTGTCACCTCACATTGCGGGCGTGGGAGACAT GAAACATTACTTGTGTGGCTACTGCGCGGCCTTCAACAACGTGGCGATCACTTACCCGGTTCAGAAGATC CTCTTCCGGCAGCAGCTTTACGGCATCAAGACCCGGGATGCAGTCCTGCAGCTGAGGAAGGATGGGTTCC GCAACTTGTACCGCGGAATCCTGCCCCCGCTGATGCAGAAGACGACCACGCTGGCACTTATGTTTGGTCT GTATGAGGACCTGTCGCGCCTGCTCCACAAGCACGTGAGCAGCGCTCCCGAGTTTGCCACCCGTAGCGTG GCGGCGCTGCTGGCCGGGACGACGGAAGCCATTCTCACTCCATTCGAAAGGGTTCAGACTTTGCTTCAGG ACCACAAACATCACGATAAGTTCACAAACACTTACCAGGCCTTCAGGGCGCTCAGATGCCATGGGATTGC AGAGTATTACCGAGGCATGGTGCCCATCCTTTTCCGGAATGGGTTCGGCAATGTCCTTTTTTTTGGCCTT CGAGGGCCCATTAAGGAGAGCCTGCCTACTGCCACTACATACAGCGCCCACCTGGTCAACGACTTCATCT GTGGAGGTGTACTCGGGGCCGTGCTGGGCTTCTTAAGCTTCCCCATTAACGTTGTCAAAGCTCGAATACA GTCTCAGATTGGCGGGCCATTCCTGTCTCTCCCCATGGTCTTCAAGACTATCTGGATAGAGCGGGACAGG AAGCTGATCAATCTCTTCAGAGGTGCACATCTGAATTACCACCGCTCTCTCATCTCCTGGGGAATAATCA ATGCCACTTACGAGTTTTTGTTAAAGATTGTATGATGAAATCATTATGTGAGATGTTATTCTCATCTAAC TAGACCTAAGGAAAATGCAATTTGGCTTTGTTCCTAATTGGCTTAAGTACAGTTTGGTGTCCAAGACCAA GCCATAACTTGCAGACAGAGCACTGACAAGAACAGAGTCCAGGTTTCAGTGGGCAGGTGTCAGGGTTCGG GTGTGGTCATTACTCCAGAGCTTTAGGCTGCTGTTGCCCGGGAACCAGCCTGCACCGCTGGGCTGTGGCC TCCGCAGCCAGAGCGCGTGCTTCGCCCCCAGCCCGGGGCATCTCAGGCTCATTCTCACCTCATTTACTTT GTCTCTGCTCTTAAGAGGGCCATAATCATTAGACCCCAGCTCTGCACGGAGGACTGGAGCAGAGGTGCCA CTCAGTGCCATGCCTGAGCTCCATGATGAATGGTTGGGGTTAGCTTTGAAATAAGTGCTGTTGAGATAGC CGCGATCTAAACTTTCTGTTCACATTTGATAGAGTTGAGCAGCTAATGAACGCTTCCATTTTGCTGTTTG GCTTTGAACACTTTCTGTTTAATTCTGAGTCTGACTTTAAGCCCTTCCAGAATGTCCTTGTGCTGTTTTT ACTGAATTCATTGCCAAAAGATAGGGTGCCCTAGTTAAATTCCGTTGTGTGCTTCATCGTGGTAAGCTGG TCACTGCAGGCTCTGCTATTCTGCTTGCTTCTTTGTGCTACTGCTGTCGACAGCCAACCAGTGAGGACAC TGTCCCTTGTGAATAGTAACTGTCATGTCTGTCCTGCTCTCTGGTACTGGACTTTAAACAAATAAACACT TCCATAAAGTTTTATTTCAGGGGTTTTAATGCAACCCCAAGTCTTCTTTTTTTCTGGTTTCTCAGTGCTA CAGAGTGAGCTAAGGAGTGGAGGGTGCTTCCCCTCTGGCCGCCCGTCTCTTTCTCTGGGAAGAAAGATTT CAGGGTTCTGCTACTCAGGAGCCCAGTTGCCACTGTGCCGTTTAGGAATGTCTGCTCTAAGTAGCAGCCC TGACTTCTGGTCAGGTAGGATCCAACTGGCAGGAACCAGGCCAAGGAGTCACATAGCTATTTTCTGGGTA AATGCTGTAATGGAAATACCATCAGTCTGGGAAGTAGCTTAGAATTTTCAGGAAGCTGTCTGTGACCACC ACCGGTCAGCTTGGTTCCCAGGAGTCGACATAAGCAAGTCAGTTTCTTTGCTTTTTTTTTTTTTGTTTGT TTGTTTATTTTTTTAAATGGTGCTAGGGATTGAACCCAGGGCCTTGCACATCCTAGGCAAATGCTGTCCC ACTACACTCCAGCCTTCCCAGTTTTGGTTTTATTTGGTTTTATTTTATTTTGAGACAGATTCTCAACAGG TAGCCCAGGCTCGTCTTGAACTCTCCTGGCTCTGCCTTCTGAGTGTTGGGATTAAAGGCGTGAGCTGCCG GACCTGGCATCCCCAGTCCTTAAGAATAGGTTCCTTCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTTCC TTCCTTCCTTCCTTTCTTTCCAGAGCTGAGGACCTAACCCAGGGCCTTGCACTTGCTAGGCAAGAGCTCT ACCACTGAGCTAAGTCCCCAACCCCAAAGATAGGTTTCTTACTAAGTTTTTCATTGGGTAATTGCGTGTG TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTACACCTATGTACCTATAAACTGAAATCAGACCCTCAGCT GCAGTTTGAGAGAAGTTTCTGGGGAAGGTAATCCAAAACAGCTCTTTAACTACACTGAAGCTGGTTACAT AAAGCTACCATCATCCAGCTGTAACAGTTGGGAGCCTGGTAAGCATTGGGGTGGCAGTAACGGAGGTCAG CGGCTTTGGAGCGACACTGGTGGAAGTGCAGTGCGGACATTAAGGGGCAAATGTGAGGACTCGGCAGACA AGAATTTGTCTGAGATACATCTGAGTACTACTGTCTTCAGTTACAATTTGAAGGTGAGAAGTCTCAGAAA CGAGAGATGGCGCCTTGGGTCCTGACCCCTGTGTCCACGTGCAGCATTGCCCAGATGCAGCACAGGGGAG GGGAGTTAACCAGGGATTATACTTTAATTTTTGTTTGTTTTTGTTTTTTCGAGACAAGGTTTCTCTGTGT AGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTTGAACTCAGAAATCCACCTGCCTCTG CCTCCCGAGTGCTGGGATTAAAGGCGTGCGCCACCACTGCCCAGCCCAGGATTATACTTGGCCTTGTTTT CTTTACACTCCGTGCAGGTGGCGCAAGTGTCGGCTGTAAAGCGCTCCTTTGAGGGTCACCAGGCTGTTAA GGGCAAGGCTTTGGCTTTGCTGACTTAATTATGTGACATTTTAAAGCAAGATTAAACTTCCTAATGCTTT TTAACTTTTAAACACTTTGTCACCCTTAGAGCTCTTAAAGACTAGATTGCAGTGTATTACAGAGGTGGCT CTGTCTCTGATAAATTTAAGTTTGACAAAAATAAAATCAGAGCTTGTAATCCACTTTGAACTTCTATGTC AGAATGTAATTAATAGTCTTAACTAGTGCGGTGATATTTTTTAAAGGCAAAAAAGACTAAAGAACTCTGA AAAGCTTTAAAGTGGCCTTTGTGTTACATGTGTGGCACAGGTGGCTCATGAGTGCAGGACACGAGTGTCC CGTGTGTCCTGTCTGATGCCACTAGAAACCTACGAGTTTTGCATTTAACACACCAGTGCTGTAAACCCTC AGGACGGTAGCAGGGTCAACACAGCCTTTGCATTGTGGCAAATTGGGGTTTTGCTTCCTTTGTGGTTAGA GACAGATGTTTGTGCCTATCAACGTTTATCTTGTGGTTGGTCATATAATCACTTGCTATATATTGTGACC CTGTGCAATGAAGCAGAATTGTACATACATAGTATTATTTGAGTACCTGAAATTCCTAAGCTCAAAGACA ATATAGATAAACTTGTAAGGTTTAGAGGGCTGGCCTCGTGTAAGTGGTTAATAGAGAGTGAACCCTGGTT TGAGGGTGGTGAGAGCTGGGTGGATGTGGTCACCGCTGGGCCAGACTGTTGCCAGGAAGGCATTCATGCT TGGGTTCCTTATGTCAAATGTATTTTTTCTCCTATATAAATAAATGCATATCAAAAGCTTAAAAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6418170 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6418170 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7648 same embryo
	EMAGE:7647 same embryo
	EMAGE:7645 same embryo
	EMAGE:7646 same embryo
	EMAGE:7643 same embryo
	EurExpress:euxassay_005285 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source