EMAGE mouse gene expression spatial database

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EMAGE:7646

Wdr12 WD repeat domain 12 ( MGI:1927241)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005293. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005293_01	euxassay_005293_02	euxassay_005293_03	euxassay_005293_04

euxassay_005293_05	euxassay_005293_06	euxassay_005293_07	euxassay_005293_08	euxassay_005293_09

euxassay_005293_10	euxassay_005293_11	euxassay_005293_12	euxassay_005293_13	euxassay_005293_14

euxassay_005293_15	euxassay_005293_16	euxassay_005293_17	euxassay_005293_18	euxassay_005293_19

euxassay_005293_20	euxassay_005293_21	euxassay_005293_22	euxassay_005293_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7646_wholemount_strong.wlz

7646_wholemount_moderate.wlz

7646_wholemount_weak.wlz

7646_wholemount_possible.wlz

7646_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7646_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8936
Entity Detected:	Wdr12, WD repeat domain 12 ( MGI:1927241)
Sequence:	sense strand is shown >T8936 AGACACGCTTAAAATTTGGCTCAAGCTAGTTATTTTCTTTATTTTCCTAATAGATTACGAATCTAGATAG ATAAGAAACAAAAGTCTTAAGGGGACTGGATGCTTTTAGGACTACGCTTGACTAATCGATGTTTCCTAAA TCTCGGAATAGGTACTTGATGTGTGCGGGCTGGGCGCTGGTGGAAATCGAACCTCGTGGTATGTTCTGTA GGAACCTGAGGGAGCTAACTGAAGCCTAGCTCCTGCTGCGCTTGCGGCGCAGTCTACCTTAGAGCTGCTT GATTATCAACGCACACATTTTCGTGGAGAAGTCTCGTGTGTATATTCAGCCATGGCACAACTCCAGGCAC GTTTCTACAGCGAGAACAAGAAATACGCAGTGGATGATGTCCCTTTCTCAATCCCTGCCGCTGCTGAAGT TGCGGACCTTAGTAACATTATCAATAAGTTGCTGGAGACCAAAAATGAACTCCACAAGCATGTCGAGTTT GATTTCCTTATCAAGGGCCAGTTTCTTCGAGTGCCCTTGGTCAAACACATGGAACTGGAGAACATTTCAT CGGAAGAGGTTGTGGAACTAGAATATGTGGAAAAGTACACCGCACCCCAGCCAGAGCAGTGCATGTTCCA TGATGACTGGATCAGCTCAATTGAAGGGGCAGAAGAATGGATCCTGTCTGGTTCTTATGATAAGACTTCT CGGATCTGGTCCTTGGAAGGAAAGTCGATAATGACAATTGTGGGACATACAGATGTTGTGAAAGATGTGG CTTGGGTGAAAAAAGACAGTTTGTCCTGCTTACTACTGACGGCCTCGATGGACCAGACTGTTCTCTTATG GGAGTGGAATGTAGAGAAGAACAAAGTGAAAGCTCTGCACTGCTGCCGAGGGCATGCTGGGAGTGTGGAC GCCATCGCAGTCGATAGCTCGGGAGCTAAGTTTTGCAGTGGCTCCTGGGATAAGATGCTAAAGATCTGGT CTACAGTCCCTACAGATGAAGAAGATGAAATGGAAGAAGCCACAAATCGACCAAGAAAAAAGCAAAAAAC AGAACAATTGGGACTAACAAGAACACCCTTGGTGACCCTCTCTGGCCACACAGAAGCAATTTCCTCAGTC CTTTGGTCAGACGCTGAAGAAATCTGTAGTGCATCTTGGGATCACACAATTAGAGTGTGGGATGTTGAGT CTGGCGGTCTTAAGTCAACTCTGACAGGAAATAAAGTGTTTAATTGTATTTCCTATTCTCCCCTTTGTAA ACGTTTGGCATCTGGAAGCACAGACAGGCATATTAGACTGTGGGATCCCCGAACTAAAGATGGTTCTTTG GTGTCGCTGTCTCTCACCTCACACACAGGCTGGGTGACATCAGTGAAGTGGTCTCCTACCCATGAACAGC AGCTCATCTCGGGCTCTTTAGATAACATTGTGAAGCTATGGGATACAAGAAGCTGTAAGGCTCCTCTCTA CGATCTAGCTGCTCATGAAGACAAAGTTCTAAGTGTGGACTGGACAGACACAGGGCTACTTCTGAGTGGA GGAGCAGACAATAAATTGTATTCCTACAGCTATTCCCCTACCACTTCCCATGTGGGGGCTTGAAAATGAA TGGCTGAGAAGAGACTTCCTTGTTGGGTCTTGTCTTAATTCTTAGTGTCTCACTGTTATTTCATAAACTG AATCAAATTTGAGAGAGGACTTATAGTACAGCTCAGCTGGTAGAATGCTTGCCTAAAATGTGCACAGCCC TGCATGCTATCTCCAGCACTGCCCAAAGAAAAATGGGCATGGTAGCACATTCCTGTAATCCCAGTCCTAG AGGTTGGAAGCAAGAGCATCAGAAATTCAAGGTCACCCTCTGCTGTATAGTGAGCTTAAAGGCAGCCAGC CATGGTTCCCTGAGTCTCTGTCTCCATAAGAAAGAAATCAATGGATAATCCTCTGATATCATTGTCAATG CAGAGACAGGAAGCAGCCTTTTAAAATTTGGATAACAGTTCATCTTTCATAATAGCAACATTTTACTGTC TTTGTCCTTCGTGCTTATAAAATACAGTAAGTATGTGGTCTGAATTCTGTCTACATTTTACCCTATGTAG ACTCCTAAAACTTGAATTTCAGAAAGTATACTTTTTAAAGGGATATAGTACAAATGTAGTAAATTTGTTT TTTTTTGTCTTTTTGTTTTGTTTTAGTTTGACTTTTTAGTTAGCTATTTGAAGAGAAACATCTAGAAAAG ACTAATAATGCTATAGGAGCCAGGCATCATAGCACACACTTATAACCCTAGCATTTAAGAAGTAGAAGCA AAAGGCTTGGGACTTGAGTAGGATTGGCTTCATAACTAGTTCAAGTCAGGAGCAGGATGAGACTCGGTCT CAAATAAAGAAAATGAAAACTTATAGGAGGGTCATCAAAATGGCTCAGTGACTAAAGATGCTTGCTGCCA ATTCTGATAGCGTGAGTTCAGTTCTCAGGTCTGAACTCCTGCAAGCTGTCCTTTTCCCCACACACATGCA CACATACTGTGGCATGCATACAACTAACCTGTAAATAAATTTAAAGAAAAAAGAAGTTTATAAAAGTATG GGGAAAACAGTCATCTAACATTAAAAGCAAATAGTAAAAAACAACAACAACAAAACACCACACACTAAAA GGTAGTGTGTATAAGAAAATAAGACTGTTGAGTGTTCAGAGCATTAAAACAATGTAGATGTTAATTTGTC CATTCATTGAGCAGCAGGGCAGTGATGGTTCACATCTTTAATGCCAGCACCTGGGAAGCAGGTGGATGTC TGTGGGTTCAAGGATAGCCTGGTGAGTTTCAGGACAGCCAGGGATAAACAGAGAGACCCTGACTCAAACG CAATAAAAAAAAAAAATAGTCTTTCCTGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6418498 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6418498 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7648 same embryo
	EMAGE:7647 same embryo
	EMAGE:7645 same embryo
	EMAGE:7644 same embryo
	EMAGE:7643 same embryo
	EurExpress:euxassay_005293 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source