EMAGE mouse gene expression spatial database

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EMAGE:7647

Ctnna1 catenin (cadherin associated protein), alpha 1 ( MGI:88274)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005276. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005276_01	euxassay_005276_02	euxassay_005276_03	euxassay_005276_04

euxassay_005276_05	euxassay_005276_06	euxassay_005276_07	euxassay_005276_08	euxassay_005276_09

euxassay_005276_10	euxassay_005276_11	euxassay_005276_12	euxassay_005276_13	euxassay_005276_14

euxassay_005276_15	euxassay_005276_16	euxassay_005276_17	euxassay_005276_18	euxassay_005276_19

euxassay_005276_20	euxassay_005276_21	euxassay_005276_22	euxassay_005276_23	euxassay_005276_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7647_wholemount_strong.wlz

7647_wholemount_moderate.wlz

7647_wholemount_weak.wlz

7647_wholemount_possible.wlz

7647_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7647_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8933
Entity Detected:	Ctnna1, catenin (cadherin associated protein), alpha 1 ( MGI:88274)
Sequence:	sense strand is shown >T8933 CGGCGGCCGTCGGCTTGGCGCCTCAGGAGTCGTCCCGTGCTCGCCCAGTTCGCTGCAGAAATGACTGCCG TCCACGCAGGCAACATAAACTTCAAATGGGACCCCAAAAGTCTGGAGATTAGGACTCTGGCTGTTGAAAG ACTGTTGGAGCCTCTTGTTACACAGGTTACAACCCTGGTAAACACCAATAGTAAAGGGCCGTCTAATAAA AAGAGAGGCCGTTCTAAGAAGGCCCATGTTTTGGCTGCATCTGTTGAACAAGCAACTGAGAATTTCTTGG AAAAGGGGGATAAAATTGCAAAAGAGAGCCAGTTTCTCAAGGAGGAGCTTGTGGTTGCTGTAGAAGATGT TCGAAAACAAGGAGATTTGATGAAGAGCGCTGCTGGGGAGTTTGCAGATGATCCATGCTCTTCTGTGAAG CGAGGCAACATGGTCCGGGCAGCTCGAGCTTTGCTCTCTGCCGTCACCCGGCTGCTGATTCTGGCTGACA TGGCAGATGTCTACAAATTACTTGTTCAGCTGAAAGTTGTGGAAGATGGTATATTGAAACTGAGGAATGC TGGCAATGAACAAGACTTAGGGATACAGTACAAAGCTCTGAAACCAGAAGTAGATAAGCTGAACATCATG GCAGCAAAAAGACAACAGGAACTAAAAGATGTGGGCAATCGTGATCAGATGGCTGCAGCTAGAGGAATCC TGCAGAAAAATGTTCCAATCCTCTATACTGCATCCCAGGCATGTCTACAACACCCTGATGTTGCAGCCTA TAAGGCCAACCGGGACTTGATATACAAGCAGCTGCAGCAGGCCGTCACAGGAATTTCTAATGCAGCCCAG GCTACTGCATCAGATGATGCTGCCCAGCACCAGGGTGGCAGTGGCGGAGAGCTGGCATACGCTCTCAACA ACTTTGATAAACAAATCATTGTGGACCCCTTGAGCTTCAGCGAGGAGCGCTTTAGGCCGTCCCTGGAGGA GCGCCTGGAAAGCATTATTAGTGGGGCTGCCCTGATGGCTGACTCATCCTGCACACGTGATGACCGTCGG GAGCGCATTGTGGCAGAGTGTAATGCTGTCCGCCAGGCTCTACAGGACCTGCTCTCAGAATACATGGGCA ACGCTGGACGTAAAGAAAGAAGTGATGCACTCAACTCTGCAATAGATAAAATGACCAAGAAGACCAGGGA CTTGCGTAGACAGCTTCGCAAAGCTGTCATGGACCACGTATCAGATTCTTTCCTGGAGACCAATGTCCCT CTATTAGTACTGATTGAAGCTGCAAAGAATGGAAATGAGAAAGAAGTTAAGGAATATGCCCAAGTTTTTC GTGAACATGCCAACAAACTGATTGAGGTTGCCAACCTGGCCTGTTCAATCTCCAACAATGAAGAAGGCGT GAAGCTTGTCCGAATGTCTGCAAGCCAGTTAGAAGCGCTGTGTCCTCAGGTTATCAATGCTGCATTGGCG TTGGCAGCAAAGCCGCAGAGTAAACTGGCCCAAGAGAACATGGATCTTTTTAAAGAGCAATGGGAAAAGC AAGTCCGTGTTCTCACAGATGCTGTTGATGACATTACTTCCATCGATGACTTCTTGGCTGTCTCAGAGAA CCACATTTTAGAAGATGTGAACAAGTGTGTTATTGCTCTCCAAGAGAAAGACGTGGATGGGCTGGATCGC ACTGCCGGTGCCATCCGAGGCCGGGCAGCCCGAGTCATTCATGTAGTCACCTCAGAGATGGACAACTACG AGCCAGGAGTTTACACAGAGAAGGTTCTGGAAGCCACCAAGCTCCTCTCCAACACAGTCATGCCGCGCTT CACTGAGCAGGTGGAAGCAGCTGTGGAAGCCCTCAGCTCGGACCCGGCCCAGCCCATGGATGAGAATGAG TTCATCGACGCCTCCCGTCTGGTGTACGACGGCATCCGGGACATCCGGAAAGCAGTGCTGATGATCAGGA CCCCCGAGGAGTTGGACGACTCTGACTTCGAGACTGAAGACTTTGATGTCAGAAGCAGGACCAGTGTCCA GACAGAAGATGACCAGCTGATAGCTGGCCAGAGCGCCCGGGCGATCATGGCTCAGCTTCCTCAGGAGCAA AAAGCAAAGATTGCAGAACAGGTGGCCAGTTTCCAGGAAGAGAAGAGCAAGCTGGATGCTGAAGTGTCCA AGTGGGATGACAGTGGCAATGACATCATTGTGCTGGCCAAGCAGATGTGCATGATCATGATGGAGATGAC CGACTTCACCCGAGGCAAAGGTCCACTCAAAAACACCTCTGACGTGATCAGTGCTGCCAAGAAAATTGCG GAGGCGGGATCCAGGATGGACAAGCTCGGCCGGACCATCGCAGACCATTGCCCAGACTCGGCATGCAAGC AGGACCTGCTGGCCTACCTTCAGCGCATCGCTCTCTACTGCCACCAGCTCAACATCTGCAGCAAAGTCAA GGCTGAAGTACAGAACCTTGGTGGAGAGCTGGTCGTCTCTGGGGTGGACAGCGCCATGTCCCTGATCCAG GCGGCCAAGAACTTGATGAATGCTGTTGTGCAGACAGTGAAGGCGTCCTACGTGGCTTCCACCAAATACC AGAAGTCACAGGGGATGGCTTCCTTGAACCTTCCTGCTGTGTCATGGAAGATGAAGGCCCCTGAGAAGAA GCCGTTGGTGAAGAGAGAGAAGCAGGATGAGACGCAGACCAAGATTAAACGAGCTTCTCAGAAGAAACAC GTGAACCCAGTGCAGGCCCTGAGCGAGTTCAAAGCCATGGACAGCATCTGAGCCTGCACAGCCCATGGGG TTCTGAAAAAAACCAGTCACATGCTTCACTCAAATGTATTTGCTAAGTAGAGAACACTGTCTTATTCCAC AGGGAGGTAGCAGATTTCAGACTCAACCAGGTGGTGAATTTTGCAAGGACATGTTTGTGTTAAAGCTGGT TAGAAAAAAAAAGCTTTTAGAATTCAAAAATATATTTCTAGCTATATTTTTATAAGTTTAATGAAAATAA CAGTTCTGTAACCAAAGAGTACCACATTAACTTCTTGGTCACATTTCTGAGTAGGCTGAAGTGCCTGCTT TCTAGGTAGGGGTGAGCGCCTGAGGGGAGAGGTTGCTTTTTAGCCCTACTGTTGGGCAGATGGAATTAAA TGACCAAGGGTGGCCCAGTGTAAATCACAGGGCACCTTAGACGGTCTGGAGAAGGAAGGTACCAGAAACC TCTTCTGAGGAATAAACTATAGGAGACAATAAAATATAACACAGTACCCAGGAAGTTGATTGCTTTCTTT ATGAAGCAGAAGGGGAATTATGACGCATGGCTACAGTTACTAATGGACTCTGCTGCAGAACATTAATGGA GTTGCTTTATTCGGGCTCGTGTGTTGTGATGCTGTAATCAGAACATGCTTCCCTGTTATTCCAGCTTGAA ATGCAAATTCACCATCGGGCTCATTTCTAATCGTCGCAGTGTTTCCTGCTTTGGGCCAGCAAAGAAGCCT GATGACTGCATAGGGTTTGCTTAGGCAATACTTAGACGCACCGTGTTTGTGACTACTACAGCAATCACCA TAGCTACGAGATGAGATTTTACTGTCTTATTTAAATTTTATGAATCTGTCTGTTTTTTACACTAATTTTC CCAATAAAGTCCATTAAGAACCAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6418358 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6418358 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7648 same embryo
	EMAGE:7645 same embryo
	EMAGE:7644 same embryo
	EMAGE:7646 same embryo
	EMAGE:7643 same embryo
	EurExpress:euxassay_005276 same experiment
	MGI:4824106 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source