EMAGE mouse gene expression spatial database

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EMAGE:7645

Vwa5a von Willebrand factor A domain containing 5A ( MGI:1915026)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005283. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005283_01	euxassay_005283_02	euxassay_005283_03	euxassay_005283_04

euxassay_005283_05	euxassay_005283_06	euxassay_005283_07	euxassay_005283_08	euxassay_005283_09

euxassay_005283_10	euxassay_005283_11	euxassay_005283_12	euxassay_005283_13	euxassay_005283_14

euxassay_005283_15	euxassay_005283_16	euxassay_005283_17	euxassay_005283_18	euxassay_005283_19

euxassay_005283_20	euxassay_005283_21	euxassay_005283_22	euxassay_005283_23	euxassay_005283_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7645_wholemount_strong.wlz

7645_wholemount_moderate.wlz

7645_wholemount_weak.wlz

7645_wholemount_possible.wlz

7645_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7645_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8935
Entity Detected:	Vwa5a, von Willebrand factor A domain containing 5A ( MGI:1915026)
Sequence:	sense strand is shown >T8935 TTGCTCTACTCTGCTCTCTCTGGGAAGAATCCTACTGATCCCTCTAAAAGAAGCGTGCAATCACCATGGA GCATCACTGTGGTCTAATCACTAGCAACAAAGAGACAGTACCACTGAAAAATATCTCAGTAACATTGTCC ATCAATGAATTTGTGGCTGCTGTGGTGGCAACCTTAAATTACGAGAATGAGGAGAAAGTCCCATTAGAGG CGACCTTTGTGTTTCCTATGGATGAAGACTCTGCTGTCTACAGCTTTGAAGCCTTGGTGGATGGGAAGAA AATTGTGGCAGAGTTGCAGGATAAGATGAAGGCTCACAGCGAGTATGAGGAAGCCCTCTCCCAGGGCCAC CAAGCATACTTATTGGAAGAGGATGACTATTCCAGAGATGTCTTTTCTTGCAACGTGGGGAACCTGCAGC CTGGAGCTAAGGTTGCAGTTACCCTGAGGTATGTGCAGGAACTGCCCTTGGAAACAGATGGGGCCCTGCG CTACCTGCTCCCAGCAATCTTGAATCCAAGATATCAGCTCTCTGAACAGTCAGCAAACAGTTGCTTGAAC ATCCAGAAACCCACAGTCCCTTTGGAAGACCTGCCTTACACACTCAACATGACTGCCACCATCACTTCCC AGCATGGTATTGAAAGGGTCCAGTCCAACTGCTCTTTGAGCCCTATTCAGTACCTTACAGATGACAAGAC TTCTGCTCAGGTTTCCTTGACTGAAGGGCACAAGTTTGACCGGGATGTGGAACTCCTGATTTACTACAAT GAAGTGCACAGCCCCAGTGTAGCTGTGGAAATGGGGATGCTGGACATGAAGCCAGACAGTTTGATGGGAG CTCCTTCTGCAATGGTGAGTTTCTACCCAGATATCCCAGAAGTGGAAGCCTCAAAGGCCTGTGGAGAATT TGTGTTTCTCATGGACCGTTCAGGAAGTATGGACTCCCCCATGAGCACAGAGAACAATTCTCAGCTACGC ATCGAGGCTGCCAAGGAAACTCTGTTGCTGTTGCTGAAGAGTTTGCCTATGGGTTGTTATTTTAATATCT ATGGATTTGGATCTTCCTATGAGAAATTCTTTCCGGAGAGTGTGAAGTACACTCAGGATACAATGGAGGA CGCAGTGAAAAGAGTGAAGGCTTTAAAAGCCAACTTAGGGGGTACTGAAATCTTGACACCCCTCTGCAAC ATTTACAAGGCATCCTCCATTCCTGGTCATCCCCTACAGCTCTTTGTCTTCACGGATGGAGAAGTGAGTG ACACATTTAGTGTCATTAGAGAAGTTAAGTTAAACAGCAAGAAACACAGATGTTTCTCTTTTGGAATTGG ACAAGGAGCCTCGACCAGTTTAATCAAAAATATCGCCCGGGTATCAGGGGGTACTGCAGTGTTTATCACA GGCAAGGACAGGATGCAGACCAAGGCTCTTGGGTCTCTCAAGTTTGCTCTACAGTGTGCAGTGGATAACA TCTCTCTAAGTTGGGATTTGCCTCCTGGTTTGTCTGTTAAAATGCTTTCTCCAGAGCAACTTACCATCTT TAGGGGTCAGAGGTTAATCATCTATGCACAACTGACTGGGCTTATGCCTCAAGTGGAGAGCACAGGAGCA GTGTGCCTCAAGCACATCCTCCAGGGTAGGAGTCTGGAAAACAGGGTGACATTTTCCCTGCAACCCAAGG CAAATGACAATTTCACCATTCATCGGTTGGCTGCAAAGTCCTTGATTCAGACTAAGGATTTTGGATCCCA GGAGGCCTCAAAAGAAGAAAAAGAAGATGTGATGAACATCAGCCTTCAGTCTGGAGTCCTTAGCTCCTTC ACAGCCTTCATTGCCATAAACAAGGAGCTGAACAAACCAGTGCAGGGGCCTCTGGCCCACAGAGTGATTC CTAGGCCAGTGATGGCTGGATCTAGTAGTATGCGGTTCTACTCCAGTTTCTCTGGTGGCTTTAAAGGCCC ACAGCCAAGAAGTTTACGTGTACCAGCATATGATTTATGTTCAGCAGAAAGTGCTAATCTTGTCCTCAAA AAATCTGCGTGTTCTGCTATACAGAAGAAAAAGACAAATTCTTCTACCAATAAAAGCAACTTGAAAAAGG AACACAAAGCATTTGGAGAGAATGCTGTGGTGCAGCTGATTTCTCTCCAAAAGGCAAATGGCTCCTGGGA GCTAGATGAAGATTTGACCAAGATCCTGGGCACTAAGTCAAAAGACATCAAGGCTGCAAACCCTGCCAAG CATGAGGACCCCTCAGCCTGGTCCACAGTGCTGGCTGTGGTCTGGTTGCATGCCAACGGCACAGACTTAA AGTGTGAATGGGAGCTTCTGGAAAGGAAGGCTGTGGCCTGGCTTCATGACCATGCAGGTTCCTCCATCCC CATGCTTGTGCAAGCTGCCAATAGTCTCCTGAAATTATCTGTGAATCCTGCTGTCTTTGGCGTCTAAACA TGTCACCCATGAAAGGAAAGCCTTTTCTGGCGACCACTGAGTCCCTTATTTTCTATCCCTATCATTTCTT TTGTCAGAAGTTAACATAGTCTCCCAACATGTTTTCTTCTTTGAGGATTTTATTTTATTCATATGCAGAA TGACACACGGAGTTTCGAGACCATTTACAATATAATTACAATATAATGATTGGCATGAGATCTATAGTAA GCATGCAGCATTAACCATGAGCAATGCTTAAATTAATACCCTGCATTATTGTTATCCATTCTCTATGCAG AGCCATGTAGTATGGTCAACAAATGGCTTTAAACTCATAGAATAGCCCTACCAGTTAGCACAGACATGAT GGATGCTCCTCAGTTGAGGCAGTACTACTAGGACTATGTGTCCTGAGTACCTCATGCTGTTATGAATTTT TGCCCTGCCCATTGCCTTCACATCCCTCTTAATCTAAGGATTGTATGAAAAGTCTAAAGTGGCTTCCAGC CCCTCACTGGGGAGTATCATTGACATTAAACAATAATAATGCCTGATATCTTTTAGGCATTGTTGCTGGA GCAGATTAGTGATCCAAGTACTACCATAGGAAAGAACTTAAAAAGATGTGGATTTTCAGCAATGTTCACC ATCCTTAAAACCTTTATTTGGAAAAACAAAGTTTTTAGTAAGCCTATGTTAAGATTTTTTTTTTGATTGT TTGTTATTGATACTGTTTTAAAAAATCTTAGTGAACATTTTGAACTTCTGTAATGCACACACATATTCAT TAAACTATGGACATTTCATGGTCAGAGAACAAGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6418714 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6418714 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7648 same embryo
	EMAGE:7647 same embryo
	EMAGE:7644 same embryo
	EMAGE:7646 same embryo
	EMAGE:7643 same embryo
	EurExpress:euxassay_005283 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source