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EMAGE:7651

Zfp866 zinc finger protein 866 ( MGI:3584369)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7651 EMAGE:7651 EMAGE:7651 EMAGE:7651 EMAGE:7651
"Pseudo-wholemount" of euxassay_005302. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005302_01 euxassay_005302_02 euxassay_005302_03 euxassay_005302_04
EMAGE:7651 EMAGE:7651 EMAGE:7651 EMAGE:7651 EMAGE:7651
euxassay_005302_05 euxassay_005302_06 euxassay_005302_07 euxassay_005302_08 euxassay_005302_09
EMAGE:7651 EMAGE:7651 EMAGE:7651 EMAGE:7651 EMAGE:7651
euxassay_005302_10 euxassay_005302_11 euxassay_005302_12 euxassay_005302_13 euxassay_005302_14
EMAGE:7651 EMAGE:7651 EMAGE:7651 EMAGE:7651 EMAGE:7651
euxassay_005302_15 euxassay_005302_16 euxassay_005302_17 euxassay_005302_18 euxassay_005302_19
EMAGE:7651 EMAGE:7651 EMAGE:7651
euxassay_005302_20 euxassay_005302_21 euxassay_005302_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7651Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7651_wholemount_strong.wlz
7651_wholemount_moderate.wlz
7651_wholemount_weak.wlz
7651_wholemount_possible.wlz
7651_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7651_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8947
Entity Detected:Zfp866, zinc finger protein 866 ( MGI:3584369)
Sequence:sense strand is shown

>T8947
GGCTCTGCGGCACAGGGAGGTGCGGTGTGTTGTGCTGTGAGCGCTGCTGCCGGGACCAGTGAGGAGAGCG
CGGGCGAGTTCGGAAGCCGCGCCATGGATTTGGTGAGCTTTGAGGACGTGGCTGTGCACTTCACCCAGGA
CGAGTGGGATTTGCTGGACCCTTTCCAGAAGAGTCTGTACAGAGATGTGATGCTGGAGACCTGCAGGAAC
CTCGCTGCGATAGGGTATATATGGGAAGAACAGAATGTTGAAGATGACTGCAAAAATTTTGGAAGAATTC
AAAGGCATGTCATATCTGATTCTGAATACTTACTGGATGAGCAGGAGGGATATGGAAACAAGCCATACGA
TTCTAGTTCTCTCACAAGTATTCAAGGACACATGGGAGCGCACACTGTGAATGGACCTTGTGAATGTGAG
GTATGTTTAAAATCCTTTGGTTTTCCAAGTGCATTTGGAATGTGTCACCAGCCCCACAGTGGACAAAACG
TTTGTGACTACAAGGAATTCGGAAAGGCTTCTGGTTACCACAGTTCACTTTACACACATGGAGGGACTGA
CACTGTAAGAAAATGTTACTCGTGTAACCAGTGTGGGAAAGCTCTGAGTTCTTCCAGTTCCCTTCAAAGG
CATGAGAGAATTCACACAGGAGAGAGACCCTATAAATGTCAGCAGTGTGGGAAAGCTCTGAGTTCTTCCA
CTTCACTTCAAAGGCATGAGAGAATTCACACAGGAGAGAGACCCTATAAATGTCAGCAGTGTGGGAAAGC
CTTTAGATGTCACAGTGCCCTTCAGTTACACGAAAGAATTCATACTGGTGAAAAACCCTATGAATGTCAG
CAGTGTGGGAAAGCCTTCACATGTCACAGTTACCTTCGATTACATGAGAGGACTCATACCGGAGAGAAAC
CTTATGAATGTAAGCAGTGTGGGAAAGCCTTCAGATGTCAAACGTCCTATCATAGACATAAAATTATTCA
TGGTGGAGAAACATTCTATGAATGTAAGCAATGTAGTAGGCTCTTCATTTATCCCTCTTTACTTCAAATG
CATGAACGAACTCACACGAGTGAAAGACCATATAAATGTAAGGAGTGTGGAAAGTCCTTCCTTTATCCCT
CTTTACTTCAAATGCATGAACGAACTCACACAGGTGAAAAGCCCTATGAATGTAAGCAATGTGGTACAGC
CTTTAGACATCACTCTTCCCTTCGGTTGCACGAAAGAACTCACACAGGAGAGAAGCCCTATGAATGCCAG
CAGTGTGGGAAAGCCTTCACGCGTCACACTTCCCTTCGGTTACATGAGATAACTCACACTGGAGAGAAAC
CTTACAAATGTAAAGAATGTGGGAAAGCCTTCAGACATCATTCTTCTCTTCGATTGCATGGAAGAAATCA
TAGTGGAGAGAAACCCTATGAATGTAAGCAATGTGATAAAGCCTTTATACGGTACAGTTACCTTCGATTA
CACGAGCGCACACACACGGGAGAAAAACCTTACGAATGCAAACAATGTGGGAAAGCCTTCAGCCGCCACA
GTTCCTTTCAGAGACATAAATCTATTCATGCTGTGGAAAACCCCTATGAGTGTCAGCAGTATCTAATTCC
TTTACCCTTATTTCCTCCAAATACATCAGACAGCTCATACTGGTGAGAAACACTATGAATGTGAACAGTG
CTACAGTGTATTCTTATCCAGTGAAAGCATGTGAGAACAGAAGCTGTCACAAGATAAGCCTTCTCCACAT
AAATGATACAGTTACAAAGGAATGCATGCCAGGCACAGTGTCTACAAATGTAAGCAGTGTGTGGCGGGCC
CTTTATTGGTCTTTACTTGCCTTTCAAGGAAGGAAGTTAATTCATGTTGGGGAAATGCCTTATGAATGGA
AGCAATATGGTGAGTCTTAGCCCTGTTTGCTTCAAACACATGAAACACTTAAGGTGCAAAATGCTGTGAC
TGTCAACAGCTGCAGCCTTCCAGAGTTGCAGGTCCCTTTGGTGTCGTGAAGGACATCTTACTGGAGGAAA
AAACCCTTGCTGTTTAGATGTCATTGCTCCCATAGATTCCATGAACAACTTCTGTTGGAAACAATGTCTG
TGAGTGGCAACAAAGTTGTAACACCTTCAAGCTCAGCTCCTTTCAAAGATGTACAAATACTAATGGTGGG
ACAAAGCCTATCCAAAGAAAAATGGTGTTGGTTTTGGTTGTCAGTAGATGGACAGACATTTCTGTCAATA
GTGCAGCTGCCTGTAAGTTGCCCGTGCTCAATAAGCCCAATTAAAGTCAGTGGCTCCCCAGGCTGCAAAA
AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6419770 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6419770 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7654 same embryo
 EMAGE:7652 same embryo
 EMAGE:7655 same embryo
 EMAGE:7650 same embryo
 EMAGE:7653 same embryo
 EurExpress:euxassay_005302 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS