EMAGE mouse gene expression spatial database

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EMAGE:8179

Usp25 ubiquitin specific peptidase 25 ( MGI:1353655)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005253. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005253_01	euxassay_005253_02	euxassay_005253_03	euxassay_005253_04

euxassay_005253_05	euxassay_005253_06	euxassay_005253_07	euxassay_005253_08	euxassay_005253_09

euxassay_005253_10	euxassay_005253_11	euxassay_005253_12	euxassay_005253_13	euxassay_005253_14

euxassay_005253_15	euxassay_005253_16	euxassay_005253_17	euxassay_005253_18	euxassay_005253_19

euxassay_005253_20	euxassay_005253_21	euxassay_005253_22	euxassay_005253_23	euxassay_005253_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8179_wholemount_strong.wlz

8179_wholemount_moderate.wlz

8179_wholemount_weak.wlz

8179_wholemount_possible.wlz

8179_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8179_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 11 13 14 15 moderate expression: see section 12

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 16 17 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 09 17

dorsal root ganglion

strong

regional

strong expression: see section 09 10 15 16 17 18

naris

strong

regional

strong expression: see section 11 15

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 11 14 15

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 09 10 11 12 13 14 15 16 17 18 19 21 22 23 24 moderate expression: see section 08 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9109
Entity Detected:	Usp25, ubiquitin specific peptidase 25 ( MGI:1353655)
Sequence:	sense strand is shown >T9109 GCGACAGGCGGGGCCGCCGCGGGGGCCATGACCGTGGAGCAGAATGTGCTGCAGCAGAGCGCGGCGCAGA AGCACCAGCAGACATTTTTGAATCAGCTGAGAGAAATTACTGGGATTAATGACGCCCAGATACTCCAACA AGCCCTGAAGGATAGTAACGGAAACTTGGAATTAGCAGTAGCCTTCCTCACTGCAAAGAATGCCAAGACC CCCCCACAGGAGGAGACAGGCTATTACCAGACAGCGCTTCCAGGCAACGACAGGTACATCAGCGTGGGAA GCCAGGCAGATGCAAATGTAATTGATCTCACTGGAGATGACAAAGATGATCTTCAGAGAGCGATTGCCTT GAGTTTGGCGGAGTCAAACAGGGCATTCAGGGAGACGGGCATAACCGATGAAGAGCAGGCCATCAGTAGA GTTCTTGAAGCCAGCATAGCAGAAAATAAAGCATGCTTGAAGAGAACACCTATAGAAGTTTGGAGGGATT CTCGAAACCCCTATGACAGAAAAAGACAAGAGAAAGCTCCAGTCGGTCTGAAGAATGTCGGCAACACCTG CTGGTTTAGTGCAGTTATTCAGTCATTATTCAATCTTTTGGAGTTTAGAAGATTAGTTCTGAATTACAAG CCTCCATCAAATGCTCAAGATTTACCCCGAAACCAAAAGGAACATCGGAATTTGCCTTTTATGCGGGAAC TGAGGTATCTGTTTGCACTTCTTGTTGGTACCAAGAGGAAGTATGTTGATCCGTCCAGAGCAGTTGAGAT TCTGAAGGATGCCTTCAAATCCAATGACTCACAGCAGCAAGATGTGAGTGAGTTTACACACAAGTTATTG GACTGGTTGGAAGATGCCTTCCAAATGAAAGCTGAAGAGGAAACGGATGAAGAGAAGCCAAAGAACCCTA TGGTAGAGCTGTTCTATGGAAGATTCCTAGCCATGGGAGTGCTTGAAGGAAAAAAATTTGAGAACACTGA AATGTTCGGTCAGTATCCACTTCAGGTGAACGGGTTCAAAGACCTGCATGAGTGCCTGGAGGCCGCCATG ATCGAAGGGGAGATCGAATCCTTACACTCCGATAACTCAGGAAAGTCAGGCCAGGAGCATTGGTTCACTG AATTGCCGCCGGTATTAACATTTGAACTGTCAAGATTTGAATTTAACCAGGCATTGGGAAGACCAGAAAA AATTCACAATAAACTAGAATTTCCCCAAGTTTTATATCTGGACAGGTATATGCACAGAAATAGAGAAATA ACAAGAATTAAACGTGAAGAGATCAAGAGACTTAAAGATTACCTCACAGTATTACAACAAAGATTAGAGA GATATTTAAGCTATGGTTCCGGTCCCAAAAGATTCCCATTGGTAGATGTTCTACAGTATGCATTGGAGTT TGCCTCCAGTAAACCTGTGTGCACTTCCCCCGTTGATGACATCGATGCTAGTTCCTCAGCAAGTGGCCCC CTACCATCACAGTCCTTACCAAGCACAACAGAACAGCAGGGACCCTGTGCTTCAGATCTGCCCGGCTCCT CCTCACCCGCATCAGGTGCCGCCCTGCCCTTGAGGTCAGTGATTCACAAACCCTTCACTCAGTCTCGGAT ACCTCCAGATTTGCCCATGCATCCGGCACCAAGGCACATCACAGAGGAAGAACTGTGCGTGCTGGAGAGC TGTTTACATCGCTGGAGGACGGAAATAGAAAATGACACGCGAGATCTGCAGGAAAGCATATCCAGAATCC ATCGAACAATTGAACTCATGTACTCGGACAAATCCATGATCCAAGTCCCTTACCGCCTCCATGCCGTCCT AGTTCATGAGGGCCAGGCTAATGCCGGCCACTACTGGGCGTACATCTTTGACCACCGGGAGAGCCGGTGG ATGAAGTATAATGATATTGCTGTGACCAAGTCTTCATGGGAAGAGCTAGTGAGGGACTCTTTCGGTGGTT ATCGAAATGCCAGTGCATACTGCTTAATGTACATAGACGACAAGGCACAGTTCCTAATACAAGAGGAGTT TAATAAAGAGACTGGGCAGGCCCTTGTTGGAATGGAAACCTTACCTCCAGACTTGAGAGACTTTGTTGAG GAGGACAACCAGCGCTTTGAGAAGGAACTGGAAGAATGGGACACGCAGCTTGCCCAACGATCACTGCAAG AAAAGCTGCTAGCTGCGCCGAAACTGCGGGAAGCAGAGGCTTCGGCCACCACAGCACAAGCAGGAGGCGC AGACTATCTAGAGCAGCCATCAAGAAGTGACTTGTCAAAGCACTGGAAAGAAGAAACGCTCCGAGTGATT GCCAAGGCGTCACATGATCTTGAAGATAAAGGGCCAGAGACAGTTTTGCAGTCGGCAATTAAATTAGAGT ATTCTAGGCTGGTTAAGTTGGCCCAAGAAGATACTCCACCAGAAACAGATTATCGTTTACATCATGTCCT GGTCTACTTTATCCAGAACCAGGCACCAAAGAAAATCATTGAGAAAACATTACTCGAACAATTTGGAGAT AGAAATTTGAGTTTTGATGAAAGGTGTCACAACATAATGAAAGTTGCCCAAGCCAAACTGGAAATGATCA AACCTGAAGAAGTGAACTTGGAAGAATATGAGGAGTGGCATGCAGATTATAAGAAATTCCGAGAAACAAC TATGTATCTCATAACTGGACTAGAAAACTTTCAGAGAGAAAGTTATATAGACTCCTTGCTGTTCCTTCTC TGTGCTTATCAGAATAACAAAGAGCTCTTGTCTAAGGGTCCGTACAGAGGCCACGATGGGGAGCTGATAT CACATTACAGAAGAGAGTGCTTACTGAAATTAAACGAGCAAGCGGCCGAGCTGTTTGAGTCTGGAGAGGA CGGAGACGTGAACAATGGCTTAATTATTATGAATGAGTTTATCGTTCCCTTTCTGCCCTTGTTGCTGGTG GATGACATGGAAGAGAAAGATATCCTTGCTGTGGAAGACATGAGGAATCGCTGGTGTTCATACCTGGGGC AAGAAATGGAAGCAAACCTCCAAGAAAAGCTCACGGATTTTCTGCCAAAACTGCTTGATTGTTCTACAGA GATTAAAGGTTTCCACGAGCCACCAAAGTTACCTTCCTACTCTGCGCACGAACTCTGTGAGCGATTCGCC CGGATCATGTTGTCCCTCAGTCGTACGCCTGCTGATGGAAGATAAACTGCCCGGACTTCCCAGGGTCACC ATAGAAACTCTGTTCTCACCCCGGCTACCTGCCGCGGGGATTGCTTGCTGCTGCTAGAGTTTTTAACTTT TTATTTTAATAACTGCAAAAGACAAAACGACTATGCCGACCTTAGCCAAGACTGCAAACAATAAACCCGA GAATCGCATGGCACTCACCTTGTTTTATCCGAAACCAATGTATAATTACAAATGAATTGATTTCTTGTGG CAAAAGTATGCTTTTGCTGCCTTCTATTGCAGTATTGCCTCTCAGTTTTCTTTACAAACAGCTTTCCATT TAGTCTGGGACCTCCAAGACTAAGCCATTAAGTGTTTAGCACTGTCTATGATACATAATATTTGGTAGCT TGTAAATGAAATCAAAGAATAAAGTTTTATTTATGGCTACCTAAGTGTTTGTAAGCAGGTATATTGTATA ATGGTGTGTATTTTAGTTATATACATGAGTTTAGCCATGGGGTGGACATTGGATGATGCATACAATAATA GCCCTTTTGTGTTAGTATAATGGAACCATATTGGAAATGTACTGAGTGTTTAGCACTTCACTGTGTCTAT ATCCACTGAGTTCTTGGACCTAAACATGTTATGTTCAGTCAGATTTCACTAAAGTGAGCGACTGGGTTTT AAGTCAAACTGCACTGAAACTTTTAACCTTTACTTAGATTAAACGTTTGTTCTTTTCTGTATGTATAAAT AATACAAGGTGATTATTTCAAGAGAAACCTGAAAAAGTACTTGAATAAGGGCTATTGTGAAATTTAAAAA GAAAAAAAATGTGCACATATACACACACATACATGCACATACATGTATTCTTCATAATGGAGGGCAATGT TTTTCATTATATAATTATTCTATTTTTGTAAATTATATACTACTTTAATTGAGAAATGTCCTCTACTAAT CAATGCTGTGAAATGAAATTGATACTGTTCAACTAGGAATTATAAGAATCTTAACTTTTTATTTGCCTCC TTTTTTTTTTCCAAAAAAAAAAAAAAAAAAAAACCGTAAAAGACGTAGACTATTTTATGAATGGAACTAC TGTTTTAAGATCAATGAAGTGATGTTGTGAATGAAAATTTAGATTCATGCTTTAAAGGTAATCATGTTAC CACCGATCTATTTTTGAATTTATAAAAATTCTTTATAACTGATACTTTGTTAGCATAGTAAAATATATCC TTAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6822095 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6822095 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8182 same embryo
	EMAGE:8180 same embryo
	EMAGE:8183 same embryo
	EMAGE:8184 same embryo
	EMAGE:8181 same embryo
	EurExpress:euxassay_005253 same experiment
	MGI:4829134 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source