EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:8183

Pafah1b3 platelet-activating factor acetylhydrolase, isoform 1b, subunit 3 ( MGI:108414)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005248. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005248_01	euxassay_005248_02	euxassay_005248_03	euxassay_005248_04

euxassay_005248_05	euxassay_005248_06	euxassay_005248_07	euxassay_005248_08	euxassay_005248_09

euxassay_005248_10	euxassay_005248_11	euxassay_005248_12	euxassay_005248_13	euxassay_005248_14

euxassay_005248_15	euxassay_005248_16	euxassay_005248_17	euxassay_005248_18	euxassay_005248_19

euxassay_005248_20	euxassay_005248_21	euxassay_005248_22	euxassay_005248_23	euxassay_005248_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8183_wholemount_strong.wlz

8183_wholemount_moderate.wlz

8183_wholemount_weak.wlz

8183_wholemount_possible.wlz

8183_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8183_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9089
Entity Detected:	Pafah1b3, platelet-activating factor acetylhydrolase, isoform 1b, subunit 3 ( MGI:108414)
Sequence:	sense strand is shown >T9089 CCGGAGTTTTCTGGCCCTGCAGCAAATGATTTCCTCCTCGGACGGAGGGCGCTAGATTGTCCCTGGTCCC TGTCGGCTTTACAGGGGGCTGCGGTGCTGAGGATGAGTGGGGAAGGGGAGAACCCGGCCAGCAAGCCCAC GCCTGTGCAAGACGTGCAGGGTGACGGGCGCTGGATGTCTCTGCACCATCGGTTTGTAGCGGACAGCAAA GACAAGGAACCCGAAGTAGTCTTTATCGGGGACTCCTTGGTTCAGCTAATGCACCAATGTGAGATCTGGC GGGAGCTCTTCTCACCTCTGCATGCACTTAACTTTGGCATTGGTGGTGACAGCACACAGCATGTACTCTG GCGGCTTGAGAACGGGGAGCTGGAGCACATCCGGCCCAAGATTGTGGTGGTCTGGGTGGGCACCAACAAC CATAGCCACACAGCAGAACAAGTGACAGGTGGCATCAAAGCCATCGTACAACTGGTGAACAAGCTGCAGC CCCAGGCACGGGTGGTTGTGCTGGGCCTGCTTCCGAGAGGCCAGCACCCCAACCCACTTCGAGAGAAAAA CCGACAGGTAAACGAGCTGGTTCGGGCAGCACTGGCTGGCTACCCCCGAGCCCACTTCTTAGATGCAGAC CCTGGGTTTGTGCATTCTGATGGCACCATAAGTCACCATGACATGTATGATTACCTACACCTGAGCCGCC TGGGGTACACACCTGTCTGCCGGGCCCTGCACTCCTTGCTTCTTCGTCTCTTGGCCCAAGATCAGGGCCA AGGCATCCCTCTGCCAGAGACAGCATCCTAAGCATCTTTCCTTCCCTTGATATTAAATGCTTCTTCTCCT CAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6820858 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6820858 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8182 same embryo
	EMAGE:8180 same embryo
	EMAGE:8184 same embryo
	EMAGE:8179 same embryo
	EMAGE:8181 same embryo
	EurExpress:euxassay_005248 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source