EMAGE mouse gene expression spatial database

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EMAGE:8180

Hspa1a heat shock protein 1A ( MGI:96244)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005247. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005247_01	euxassay_005247_02	euxassay_005247_03	euxassay_005247_04

euxassay_005247_05	euxassay_005247_06	euxassay_005247_07	euxassay_005247_08	euxassay_005247_09

euxassay_005247_10	euxassay_005247_11	euxassay_005247_12	euxassay_005247_13	euxassay_005247_14

euxassay_005247_15	euxassay_005247_16	euxassay_005247_17	euxassay_005247_18	euxassay_005247_19

euxassay_005247_20	euxassay_005247_21	euxassay_005247_22	euxassay_005247_23	euxassay_005247_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8180_wholemount_strong.wlz

8180_wholemount_moderate.wlz

8180_wholemount_weak.wlz

8180_wholemount_possible.wlz

8180_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8180_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

moderate

regional

moderate expression: see section 06 17 weak expression: see section 07 08 15 16

vibrissa

moderate

regional

moderate expression: see section 05 06 21 weak expression: see section 07 20

testis

moderate

regional

moderate expression: see section 05 06 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9045
Entity Detected:	Hspa1a, heat shock protein 1A ( MGI:96244)
Sequence:	sense strand is shown >T9045 TGGTTCCATCCAGAGACAAGCGAAGACAAGAGAAGCAGAGCGAGCGGCGCGTTCCCGATCCTCGGCCAGG ACCAGCCTTCCCCAGAGCATCCCTGCCGCGGAGCGCAACCTTCCCAGGAGCATCCCTGCCGCGGAGCGCA ACTTTCCCCGGAGCATCCACGCCGCGGAGCGCAGCCTTCCAGAAGCAGAGCGCGGCGCCATGGCCAAGAA CACGGCGATCGGCATCGACCTGGGCACCACCTACTCGTGCGTGGGCGTGTTCCAGCACGGCAAGGTGGAG ATCATCGCCAACGACCAGGGCAACCGCACGACCCCCAGCTACGTGGCCTTCACCGACACCGAGCGCCTCA TCGGAGACGCCGCCAAGAACCAGGTGGCGCTGAACCCGCAGAACACCGTGTTCGACGCGAAGCGGCTGAT CGGCCGCAAGTTCGGCGATGCGGTGGTGCAGTCCGACATGAAGCACTGGCCCTTCCAGGTGGTGAACGAC GGCGACAAGCCCAAGGTGCAGGTGAACTACAAGGGCGAGAGCCGGTCGTTCTTCCCGGAGGAGATCTCGT CCATGGTGCTGACGAAGATGAAGGAGATCGCTGAGGCGTACCTGGGCCACCCGGTGACCAACGCGGTGAT CACGGTGCCCGCCTACTTCAACGACTCTCAGCGGCAGGCCACCAAGGACGCGGGCGTGATCGCCGGTCTA AACGTGCTGCGGATCATCAACGAGCCCACGGCGGCCGCCATCGCCTACGGGCTGGACCGGACCGGCAAGG GCGAGCGCAACGTGCTCATCTTCGACCTGGGGGGCGGCACGTTCGACGTGTCCATCCTGACGATCGACGA CGGCATCTTCGAGGTGAAGGCCACGGCGGGCGACACGCACCTGGGAGGGGAGGACTTCGACAACCGGCTG GTGAGCCACTTCGTGGAGGAGTTCAAGAGGAAGCACAAGAAGGACATCAGCCAGAACAAGCGCGCGGTGC GGCGGCTGCGCACTGCGTGTGAGAGGGCCAAGAGGACGCTGTCGTCCAGCACCCAGGCCAGCCTGGAGAT CGACTCTCTGTTCGAGGGCATCGACTTCTACACATCCATCACGCGGGCGCGGTTCGAAGAGCTGTGCTCA GACCTGTTCCGCGGCACGCTGGAGCCCGTGGAGAAGGCCCTGCGCGACGCCAAGATGGACAAGGCGCAGA TCCACGACCTGGTGCTGGTGGGCGGCTCGACGCGCATCCCCAAGGTGCAGAAGCTGCTGCAGGACTTCTT CAACGGGCGCGACCTGAACAAGAGCATCAACCCGGACGAGGCGGTGGCCTACGGGGCGGCGGTGCAGGCG GCCATCCTGATGGGGGACAAGTCGGAGAACGTGCAGGACCTGCTGCTGCTGGACGTGGCGCCGCTGTCGC TGGGCCTGGAGACTGCGGGCGGCGTGATGACGGCGCTCATCAAGCGCAACTCCACCATCCCCACCAAGCA GACGCAGACCTTCACCACCTACTCGGACAACCAGCCCGGGGTGCTGATCCAGGTGTACGAGGGCGAGAGG GCCATGACGCGCGACAACAACCTGCTGGGGCGCTTCGAACTGAGCGGCATCCCGCCGGCGCCCAGGGGCG TGCCACAGATCGAGGTGACCTTCGACATCGACGCCAACGGCATCCTGAACGTCACGGCCACCGACAAGAC CACCGGCAAGGCCAACAAGATCACCATCACCAACGACAAGGGCCGCCTGAGCAAGGAGGAGATCGAGCGC ATGGTGCAGGAGGCCGAGCGCTACAAGGCCGAGGACGAGGTGCAGCGCGACAGGGTGGCCGCCAAGAACG CGCTCGAATCCTATGCCTTCAACATGAAGAGCGCCGTGGAGGACGAGGGTCTCAAGGGCAAGCTCAGCGA GGCTGACAAGAAGAAGGTGCTGGACAAGTGCCAGGAGGTCATCTCCTGGCTGGACTCCAACACGCTGGCC GACAAGGAGGAGTTCGTGCACAAGCGGGAGGAGCTGGAGCGGGTGTGCAGCCCCATCATCAGTGGGCTGT ACCAGGGTGCGGGTGCTCCTGGGGCTGGGGGCTTCGGGGCCCAGGCGCCCAAGGGAGCCTCTGGCTCAGG ACCCACCATCGAGGAGGTGGATTAGAGGCCTCTGCTGGCTCTCCCGGTGCTGGCTAGGAGACAGATATGT GGCCTTGAGGACTGTCATTATTTCAAGTTTAGTACTTCACTCCTTAGTTTGTCCTGCAATCAAGTCCTAG ACTTAGGGAAACTAAACTGTCTTTCAGTTACTTTGTGTATTGCACGTGGGCTTTATCTTCCCTGTTAATT AACACTGCAAGTGTGTCTTTGTAAATATAAATAAATAAGTATATATATTCTTCAATTCAGCACTGCCCCG CTGATGTGATTTGTTTTGCAGGACAGCCAAAGCTATGTAGAGAGATATTCTGTATCAGAATACACAAAGA GACAGAGATATGTTATGAAAACATCAGGAGACTGTTGAGTTCTTTGTGTTTGGACTCTCCCCTGGGCCAC ATTGTTGATACATGCTTGTGTCGGGTCCTTCAGAGGCCAGGGCTGGATTACTGACAGCGGAGACTCTGCT GCTTCTCCTTGCGTTTATAATCTTGCATGGTGGTTGCACTGTAGGACTTGTTTCCAGGTTGGTGAACTTG GAGGTGAAGTGACAGCACCAGCATGTGTTCAGTTTTTACACAACCATCCTGAACTCGGGTCAATTTTTAC CGGTCATTTGAAAATAAACTTCAAAATCACTTGCCAGGTCTTGTGCCTGTTGTGTTTGGAGGTCAGGAGT TGCTGTGTATGACAGTTTCGGCTACGCTTGACTAACATTTTATCTGAAGACACAGACACGGGTGTGGAGC ACTCACAGGGACATTGCATTGTGATCTGCTTGTGTGAGAAAAATTAAAGTCAGAGCAAATAAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6815894 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6815894 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8182 same embryo
	EMAGE:8183 same embryo
	EMAGE:8184 same embryo
	EMAGE:8179 same embryo
	EMAGE:8181 same embryo
	EurExpress:euxassay_005247 same experiment
	MGI:4825477 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source