EMAGE mouse gene expression spatial database

EMAGE:8184

Tyrobp TYRO protein tyrosine kinase binding protein ( MGI:1277211)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005252. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005252_01	euxassay_005252_02	euxassay_005252_03	euxassay_005252_04

euxassay_005252_05	euxassay_005252_06	euxassay_005252_07	euxassay_005252_08	euxassay_005252_09

euxassay_005252_10	euxassay_005252_11	euxassay_005252_12	euxassay_005252_13	euxassay_005252_14

euxassay_005252_15	euxassay_005252_16	euxassay_005252_17	euxassay_005252_18	euxassay_005252_19

euxassay_005252_20	euxassay_005252_21	euxassay_005252_22	euxassay_005252_23	euxassay_005252_24

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Find spatially similar wholemount expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

moderate

single cell

moderate expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9043
Entity Detected:	Tyrobp, TYRO protein tyrosine kinase binding protein ( MGI:1277211)
Sequence:	sense strand is shown >T9043 CCAGGTCCCACCAGCCCCTGGACTGTGGTGTCCAGTGCATATCTGGCCACCATGGGGGCTCTGGAGCCCT CCTGGTGCCTTCTGTTCCTTCCTGTCCTCCTGACTGTGGGAGGATTAAGTCCCGTACAGGCCCAGAGTGA CACTTTCCCAAGATGCGACTGTTCTTCCGTGAGCCCTGGTGTACTGGCTGGGATTGTTCTGGGTGACTTG GTGTTGACTCTGCTGATTGCCCTGGCTGTGTACTCTCTGGGCCGCCTGGTCTCCCGAGGTCAAGGGACAG CGGAAGGGACCCGGAAACAACACATTGCTGAGACTGAGTCGCCTTATCAGGAGCTTCAGGGTCAGAGACC AGAAGTATACAGTGACCTCAACACACAGAGGCAATATTACAGATGAGCCCACTCTATGCCCATCAGCGGC CTGATGCCCGGATCCGGTCATTCCAGATGCCTACTCAACAAGCCCTCTCTGGGATCAGGACTCCCGTTGG AATACAGATCCACAGGGTACCTCCCTGAGATATCTGACATTGTACCATTTCTGTCCCCAAATAAAAGACA GAGCAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6814478 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6814478 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8182 same embryo
	EMAGE:8180 same embryo
	EMAGE:8183 same embryo
	EMAGE:8179 same embryo
	EMAGE:8181 same embryo
	EurExpress:euxassay_005252 same experiment
	MGI:4829040 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source