EMAGE mouse gene expression spatial database

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EMAGE:10775

Dazap2 DAZ associated protein 2 ( MGI:1344344)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001001. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001001_01	euxassay_001001_02	euxassay_001001_03	euxassay_001001_04

euxassay_001001_05	euxassay_001001_06	euxassay_001001_07	euxassay_001001_08	euxassay_001001_09

euxassay_001001_10	euxassay_001001_11	euxassay_001001_12	euxassay_001001_13	euxassay_001001_14

euxassay_001001_15	euxassay_001001_16	euxassay_001001_17	euxassay_001001_18	euxassay_001001_19

euxassay_001001_20	euxassay_001001_21	euxassay_001001_22	euxassay_001001_23	euxassay_001001_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10775_wholemount_strong.wlz

10775_wholemount_moderate.wlz

10775_wholemount_weak.wlz

10775_wholemount_possible.wlz

10775_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10775_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 12 13 15 16 weak expression: see section 14

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 19 20 21 weak expression: see section 10

vibrissa

moderate

homogeneous

moderate expression: see section 22 23 24 weak expression: see section 07 08 09

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 12 18

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate ventricular layer

weak

homogeneous

weak expression: see section 12 13 14 15 16 17

rest of cerebellum ventricular layer

moderate

homogeneous

moderate expression: see section 04 20 21 22 23 weak expression: see section 06 07 19

pons ventricular layer

weak

homogeneous

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

midbrain ventricular layer

weak

homogeneous

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 weak expression: see section 04 05 24

neural retina

weak

regional

weak expression: see section 01 02 03 04 05 24

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 10 11 12 13 16 17 18 19 20 21

meckel's cartilage

weak

regional

weak expression: see section 04 05 23 24

lower jaw incisor

weak

regional

weak expression: see section 12 13 17 18

lower jaw molar

weak

regional

weak expression: see section 09 10 19 20 21

upper jaw incisor

weak

regional

weak expression: see section 12 13 17

upper jaw molar

weak

regional

weak expression: see section 09 10 19 20 21

liver

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

kidney calyx

weak

homogeneous

weak expression: see section 07 08 09 10 11 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4294
Entity Detected:	Dazap2, DAZ associated protein 2 ( MGI:1344344)
Sequence:	sense strand is shown >T4294 GAGAAAAATAACCGTCCGCGACGCCGAGTGGGAGCGGACTCAGAGCCACCATGAACAGCAAAGGTCAATA TCCAACACAGCCTACCTACCCTGTGCAACCTCCTGGGAATCCAGTATATCCTCAGACCTTACATCTTCCT CAGGCTCCACCCTATACTGATGCTCCACCTGCATATTCAGAGCTCTATCGTCCAAGCTTTGTTCATCCAG GGGCTGCCACAGTCCCCACCATGTCAGCTGCATTTCCTGGCGCCTCATTGTATCTGCCTATGGCCCAGTC TGTGGCTGTTGGGCCTCTAGGCTCCACAATCCCCATGGCTTATTATCCAGTTGGTCCCATCTATCCACCT GGCTCAGCAGTGTTGGTGGAAGGAGGGTATGATGCAGGTGCCAGATTTGGAGCTGGTGCCACTGCTGGCA ACATTCCTCCTCCACCTCCTGGATGCCCTCCCAACGCTGCTCAGCTCGCAGTCATGCAGGGAGCCAACGT CCTTGTAACTCAGCGCAAGGGAAACTTCTTCATGGGTGGCTCAGATGGTGGCTACACCATTTGGTGAGGA GCCATGGCCACTCCTGTGCCGGGGAAGACATCACATACCTTCAGCACTTCTCACAATGTAACTGCTTTAG TCATATTAACCTGAAGTTGCAGTTTAGACACATGTTTTTGGGGTGTCTTTCTGGTGTCCAAACTTTTGAG GCACTTTTCAAATTTAATAAGGAACCATGTAAGGGTAGCAGCCCCTCCCTAAAGCATTCTGAGGTAAGGA GGTATCCATTCTTCAAATGAATGTGAGTGAAGCAGCCCAAGGCTCTTCCTTAATTCCTCTGGAGTGATGC TGTGCCATGCTGGTCTTTGCTTCTAGTAATAAAACATCAAATTAGGTTTGGAGGGAACTTTGATCTTCCT AAGAATTAAAGTTGCCAAATTATTCTGATTGGTCTTTAATCTCCTTAAGTTTTTGATTTATCTTACTTGG TAAATGAAATGCATTAGCTGTCTGCCTTTTCCTTCCCATCCTCTGCCCAGCCTGTCCACTGTCCCTGCTC CTCCACCCCCTCCCATCACTATCCATAGAATCAGTGGTGCAGGACCGAAAGCCAGACTCAGCCCCTTGTC TCCCTGCACCTGTCCCACTTGACATTTTTTAACTCATCTTTCATAAGGATCCTCTGAAGCCCCCTGTGTG ACCTGAGCACAGACTCCATTTCTTCAGGTTTTGTATATCTCCAGGCAAGGTGAAGGGTACCCTTCGGACC CGCCTCATAAACCATCTCTGCATCACCATGAACCCAGACTAGCTGTGGTGCCAGAAACCTGAACTTAAAC AACAGTTCTCAGTGCAAACTGTCCTAGGAAAATTTAAAGATGACAGGCAGGGGAGAAGATGCATATCAAA AGCTTGGCTTTAAAGTCTAAGGGAGTTTTAGATCAAAAACTAGCTATCACTTCCTGAGTGTCTCATGTAG CCACTCAGGCTCCCAGGAGTTTCCTGAGGTAGGTTAGTAGGGTCACGTGAATGCCTCACTATGACATGAC TTGAGTCCAGTGAGATCTCATTAGGATTAATATTTCAGGGATCCTTAATATTTTGATTTGTTTTCCGAGA TTGGATTTTATTTTATCTGCTATTTCAGTTCATCTAAATCTTGTGTTCTGTACATGTGATGTTTGACTGT ACCATCGACTGTTATGGAAGTTCAGCGTTGTATGTCTCTCTACACTGTGGTGCACTTAACTTGTGGATTT TTTATACTAAAAATGTAGAATAAAGACTATTTTGAAGATTTGAATAAAGTGATGAAGTTGCATTACACCT CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4506124 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4506124 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10778 same embryo
	EMAGE:10776 same embryo
	EMAGE:10777 same embryo
	EMAGE:10779 same embryo
	EurExpress:euxassay_001001 same experiment
	MGI:4824236 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source