EMAGE mouse gene expression spatial database

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EMAGE:10954

Ppme1 protein phosphatase methylesterase 1 ( MGI:1919840)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003617. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003617_01	euxassay_003617_02	euxassay_003617_03	euxassay_003617_04

euxassay_003617_05	euxassay_003617_06	euxassay_003617_07	euxassay_003617_08	euxassay_003617_09

euxassay_003617_10	euxassay_003617_11	euxassay_003617_12	euxassay_003617_13	euxassay_003617_14

euxassay_003617_15	euxassay_003617_16	euxassay_003617_17	euxassay_003617_18	euxassay_003617_19

euxassay_003617_20	euxassay_003617_21	euxassay_003617_22	euxassay_003617_23	euxassay_003617_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10954_wholemount_strong.wlz

10954_wholemount_moderate.wlz

10954_wholemount_weak.wlz

10954_wholemount_possible.wlz

10954_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10954_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 17 weak expression: see section 13 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 11 17 18 19 20

thyroid gland

weak

regional

weak expression: see section 13 17

vibrissa

moderate

regional

moderate expression: see section 04 05 06 07 19 20 21 22 weak expression: see section 23

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 09 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 10 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 09 19 20 21

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 12 18 19

cervical ganglion

moderate

regional

moderate expression: see section 11 18

thoracic ganglion

moderate

regional

moderate expression: see section 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 12 13 15 16 17 18 19 20

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 07 08 09 10 11 12 14 15 16 17 18 19

nasal cavity respiratory epithelium

weak

regional

weak expression: see section 11 15

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 12 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 10 11 12 15 16

bladder

strong

regional

strong expression: see section 14

bladder

strong

regional

Expression in the fundus region.

genital tubercle of male

moderate

regional

moderate expression: see section 11 15

urethra of male

moderate

regional

moderate expression: see section 12 13 14

clavicle

moderate

regional

moderate expression: see section 11 12 18 weak expression: see section 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6609
Entity Detected:	Ppme1, protein phosphatase methylesterase 1 ( MGI:1919840)
Sequence:	sense strand is shown >T6609 CTGTGACCGGTTGTACTGCACGTATCGTGGGACGGACCTTGGGCCACTGTTGTCGACGTGCGGCCTCCCT TTGATGTCGGCCCTTGAAAAAAGCATGCACCTCGGCCGCCTACCTTCTCGCCCTCCTCTACCCGGCAGCG GGGGCAGTCAGAGCGGAGCCAAGATGCGGATGGGCCCTGGACGGAAGCGGGACTTTACCCCTGTCCCATG GAGTCAGTACTTTGAGTCAATGGAAGATGTGGAAGTGGAGAATGAAACTGGCAAGGATACTTTTCGAGTT TACAAGAGTGGTTCTGAGGGTCCAGTCCTGCTGCTGCTTCATGGAGGAGGCCATTCCGCCCTTTCCTGGG CAGTGTTCACGGCAGCAATCATCAGTAGAGTACAGTGTAGGATTGTGGCTCTGGATCTGCGAGGTCACGG AGAAACTAAAGTCAAGAATTCTGAAGACCTGTCAGCAGAAACAATGGCAAAAGATGTTGGCAATGTGGTG GAAGCCATGTATGGAGACCTCCCTCCTCCAGTCATGCTGATTGGGCACAGCATGGGTGGCGCCATTGCAG TGCACACAGCCGCAGCTAACCTGGTACCAAGCCTCTTGGGCCTTTGCATGATTGATGTTGTAGAAGGTAC CGCCATGGATGCACTTAATAGCATGCAGAATTTCTTGCGTGGTCGACCTAAAACCTTCAAGTCTTTGGAG AATGCTATTGAATGGAGTGTGAAGAGTGGCCAGATTCGAAACCTTGAGTCTGCCCGTGTATCCATGGTCG GCCAAGTCAAACAGTGTGAAGGAATTACAAGTCCAGAAGGTTCCAAATCCATAGTGGAAGGAATCATAGA GGAGGAGGAAGAAGATGAGGAAGGAAGTGAGTCAGTTAACAAGAGGAAAAAGGAAGACGACATGGAAACC AAGAAGGATCACCCATACACCTGGAGAATTGAGCTGGCAAAAACAGAAAAGTACTGGGATGGCTGGTTCC GGGGCTTATCCAATCTCTTTCTTAGCTGTCCTATTCCTAAACTGCTGCTCTTGGCAGGTGTTGACAGATT GGATAAAGATCTGACCATAGGCCAGATGCAGGGGAAGTTCCAGATGCAGGTCTTACCCCAGTGTGGCCAT GCAGTCCATGAGGATGCCCCTGACAAGGTAGCTGAAGCTGTTGCCACTTTCCTGATCCGGCACAGGTTTG CAGAGCCCATTGGAGGATTCCAGTGTGTGTTTCCTGGCTGCTAGTGACCTGCTGTCTACTCCTCCCTCTA CATTGAGCTCTGTTGTAAATACATCGCACCAGAGGCCACTGTGACGCCGCTGTCTCCTCCTCTCCATCCC GCCCAGCCATGTGACACCGGCTCTTGTAGAGGGCATCCCCAGATGTCCAAACCCTTTCCTGTGTACTGTT GAAAGCATTGTTCTTCAGGGCCCTTGTCCAACAGTGGCCCGTGCAGTCTGGGGTCCACAGCTCTTCCTCT CCTTCCTGTGCTCCCTGCCTTGCCTAGGATGAAGCCTCCAGCGCTGCTCCCTGGCCCTGTTCCTGGCATA TGGCAATGTACCCCAGGCTCAGGGATCTCCCTTCCTTGAGGATGTTCTTGGCATGGTCCTGCCCTACCTC ATGGGATGGGCAATGCACACACTGGCCCTTATTTTTCCCTTTCAAATAAAACACCAGTCAGGTACCTTTA TCCCAGTCTTAACTGTCCCAAATCTGGAAGGTCCAGAGTAAGCAGGATTCAGGGAGAGGGAGTGGATAGC AAGTATCCCAAGAAACCAACCTGTAAGTCAGGTCCAGCCAGTCCAAGCACATGGCTTCCCATCTGGGTGA GCCCACTGTCCCACTCCCACATGTCTGGGCACCTGCCCTGGGCTGAGGCCAGGCTGCTCCAAGGGCCGCA TGAGCCCTAATCTGCCACAGAGCAACCCAGGTTAAACACAGCCCATGCACAAAGCCACAGGCTAAATCCT GTGGAATTGTTTTTAATGACTGAATTTAACCATTTTCATAGTTGGTTCCTGGAGGTGTGCCAAGTGCCCG CTTGCCTCTTCTAGACCCACAGCTTCTTGATCCACTTGTGGTTTCCATGTCACTAATGTAGAAACATCAT GGACTAGCATCCCCAGTCTTTGCCCTCATCCAGCTGTCGCAGTGCAGCACTGGGGCCTCCCCCTGCTGCC CATGGGGGGGGGGGGTGGGCAGCCTCCTGAAACCCATCTTTCTGTGACTGTCTTAGGTGACGTGTAGCCC TCTTCCATTTTTTCACCCAACAACTTCCTCTGTCCTGCTGCACGGTCCAGAGTCTGGGACCGACTTTGTT TCTTTGTTATTTATGATCTTGTTTAAAGAAAATAAATATCTCCCAACCTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3596696 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3596696 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10955 same embryo
	EMAGE:10953 same embryo
	EMAGE:10956 same embryo
	EMAGE:10957 same embryo
	EMAGE:10952 same embryo
	EurExpress:euxassay_003617 same experiment
	MGI:4827367 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source