EMAGE mouse gene expression spatial database

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EMAGE:11012

Mmd2 monocyte to macrophage differentiation-associated 2 ( MGI:1922354)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001134. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001134_01	euxassay_001134_02	euxassay_001134_03	euxassay_001134_04

euxassay_001134_05	euxassay_001134_06	euxassay_001134_07	euxassay_001134_08	euxassay_001134_09

euxassay_001134_10	euxassay_001134_11	euxassay_001134_12	euxassay_001134_13	euxassay_001134_14

euxassay_001134_15	euxassay_001134_16	euxassay_001134_17	euxassay_001134_18	euxassay_001134_19

euxassay_001134_20	euxassay_001134_21	euxassay_001134_22	euxassay_001134_23	euxassay_001134_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11012_wholemount_strong.wlz

11012_wholemount_moderate.wlz

11012_wholemount_weak.wlz

11012_wholemount_possible.wlz

11012_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11012_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 15 16 17 18

hypothalamus ventricular layer

strong

homogeneous

strong expression: see section 13 moderate expression: see section 12 14

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 14 15 16 17

diencephalon lateral wall ventricular layer

strong

homogeneous

strong expression: see section 13 moderate expression: see section 12 14

thalamus mantle layer

moderate

homogeneous

moderate expression: see section 13 18

cerebral cortex

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

olfactory lobe

moderate

homogeneous

moderate expression: see section 12 13 14 16 17 18 19 20

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate ventricular layer

strong

homogeneous

strong expression: see section 09 10 11 12 13 moderate expression: see section 08 14 15

metencephalon basal plate

moderate

regional

moderate expression: see section 07 17 18 19 weak expression: see section 05

pons ventricular layer

strong

regional

strong expression: see section 08 09 10 11 13 moderate expression: see section 05 06 07 15 16 17 18 weak expression: see section 19 20

midbrain ventricular layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16 moderate expression: see section 06 07 08 09

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 20 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 08 18

ventral grey horn

moderate

regional

moderate expression: see section 11 12 13 weak expression: see section 10 14 15

intermediate grey horn

moderate

regional

moderate expression: see section 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 12 16 17 18

liver lobe

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 weak expression: see section 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4233
Entity Detected:	Mmd2, monocyte to macrophage differentiation-associated 2 ( MGI:1922354)
Sequence:	sense strand is shown >T4233 GGCTCCGGAGGCAACTGATCGGGTCTGTTCAAGGCCCTGGAGCAGCCCGAGGATCTGACACCTTTCCAAC CTGCTGCGCCTCCCGAAGCGGCTCCTGGTGTTCCCGCGTCCCAGCCCTGGTCTGGGCGCTGATGGAACCG CGGCGATGTTCACTCTGGCCAGGCTGCTGGATTTCCAGAAAACCAAATATGCAAGGTTTATGAATGACCG AGTCCCGGCGCACAAGAGGTACCAGCCCACTGAGTATGAACACGCAGCAAACTGTGCCACCCATGCTTTC TGGATCATTCCCAGCATCCTTGGCAGCTCCAACCTCTACTTCCTGTCCGATGACGACTGGGAGACCATAT CTGCCTGGATCTACGGCCTTGGCCTCTGTGGCCTCTTTGTGGTATCCACCATTTTCCACACAGTCTCATG GAAAAAGAGCCACCTCAGGATGGTAGAGCACTGCCTGCACATGATCGACAGGATGGTGATCTACTTCTTC ATTGCCGCCTCCTATGCTCCTTGGCTGAACCTTCGGGAGCTGGGCCCCTGGGCCTCCCACATGCGCTGGC TGGTGTGGATCATGGCCTCCATCGGTACCATCTATGTCTTTTTCTTCCATGAACGGTACAAGCTTGTGGA GCTGCTTTGCTATGTGGTGATGGGTTTCTTCCCTGCCCTGGTCATCCTCTCCATGCCCAACACTGATGGC ATCTGGGAGCTGATGACAGGAGGGGCTTTCTACTGCCTGGGCATGGTGTTCTTTAAGAGTGATGGAAGAA TTCCCTTTGCCCATGCCATCTGGCACCTCTTTGTGGCATTTGGTGCTGGTACCCACTACTATGCCATCTG GAGGTACCTCTACCTGCCCAGCACACTGCAAACCAAGGTGTCCAAATGAGTGGCCCAGACTCCATGGGTC ATTTGGACTTTGGGATCAAAGCAACATGGGAAGTGAACTGTGCCAAGATCTGCCTGTCTTTTCCTGGATG GAATTCTTCATGGGTCTAAGGAACCTTTCAGTGACAACAAGACCACCTTTTGAATCCCCAACAAGGAAGA AAACATTTAGCCCTTTCTATGGAGGAGAAATTAATTCAGTACTGTGTTTCTACTTTAGACAGTTTTTTTT TTTTTTTTACTATAACCAATATTAACATCTTCAGAAAAGGGCCAAACAGGGAATGACCATCCAGAAGGTA ATTTTTAAAGTTCTGAGGGCCTCCCTTTCTGCTTCAGGAGAAAGTGATTAGCATTATAGATGATCTATGG GGAATATCCCTGACCATGGTAGGCATCACCAATCAATTGCAGCACGCATGTAGCAGTAGAATTCAACATA GTGTCCCATGCAGACATTACTAATTGATTCTGTGCTCATGATGGAACCTTTTGGCTGGCCTGGTGTGTTA GGAGACAGGATCACAGAATAACTATTAGAAGTCACTGTATGTAGGAGCATGCTTATCTCTGGGCACCATT ACCTTCTACTCCCTTCTGTCCATATTTGAGGAAGTTCTTTAAGTGAGCTCTTTGGAGGCAGTGCAGGGAG ACCAAGGCATACATTCCAAACAGGTCCTGACACTCTGTTCCGCCATGGTTGGCTCTTGGTTCTAGAGGAG CTGAGATCTGGGATGAAGATGCTGATACCTGGCCATTTTTTCTCATCTCAAAGAATCTGGAGAAAGGATG CTGTGTTCACCAGAGGGGCTTTTCCTCAGTGTCAGGAAACGGCTCTCGGAGGCCCCTCAGATGGCCCTAG GAGCAGGGTCTTCCTCTATCAGAATGGGTGGAATATCTTCATCCCTCAACCTCTGAAGTGACCTGTCAGC TAAGAGCATGGCTCCCAGGGGCCTGTCCTCAGCCACCTCTATTCCTGGGCTAGCCTCATCCTAAGATGCC ACATGGACACTGGCTGAGTATTGAGCAGCTGGCCTGGCTCCTGACCTGAACTGACATCTCAGACTCTCTG CAAGAGTCTGCTGGAAATCCAAGGTCTTGTTCAACAACTGCCAGCCAGATGGGTGTGGGCAAGGGTGTGC CAAGACAGGGTGCCTTATACTTTGTTCTAGTGCATTCCAAGGTGTTTCAGGGCTTCAGCACCTGGAATCC CATATGTCAAAGCCAGTATTAAATCAAGTTTATGCATTCCTCAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4502289 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4502289 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11009 same embryo
	EMAGE:11008 same embryo
	EMAGE:11011 same embryo
	EMAGE:11010 same embryo
	EMAGE:11007 same embryo
	EurExpress:euxassay_001134 same experiment
	MGI:4826401 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source