EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11066

Haus1 HAUS augmin-like complex, subunit 1 ( MGI:2385076)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001268. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001268_01	euxassay_001268_02	euxassay_001268_03	euxassay_001268_04

euxassay_001268_05	euxassay_001268_06	euxassay_001268_07	euxassay_001268_08	euxassay_001268_09

euxassay_001268_10	euxassay_001268_11	euxassay_001268_12	euxassay_001268_13	euxassay_001268_14

euxassay_001268_15	euxassay_001268_16	euxassay_001268_17	euxassay_001268_18	euxassay_001268_19

euxassay_001268_20	euxassay_001268_21	euxassay_001268_22	euxassay_001268_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11066_wholemount_strong.wlz

11066_wholemount_moderate.wlz

11066_wholemount_weak.wlz

11066_wholemount_possible.wlz

11066_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11066_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 12 13 16 weak expression: see section 14 15

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 18 19 20 21 weak expression: see section 11 17

pancreas

weak

regional

weak expression: see section 09 10 11 12 13 16 17 18

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 12 13 18

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 16

esophagus

weak

regional

weak expression: see section 13 14

lower jaw incisor

moderate

regional

moderate expression: see section 13 14 17 18

upper jaw incisor

moderate

regional

moderate expression: see section 13 14 18

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23

renal cortex

weak

homogeneous

weak expression: see section 08 09 10 11 18 19 20 21

chondrocranium

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4674
Entity Detected:	Haus1, HAUS augmin-like complex, subunit 1 ( MGI:2385076)
Sequence:	sense strand is shown >T4674 CTTTGAGATTGAGGTGGCGGGAGCGGCGGCTATGGCTGCGCCGGAGGAGAAAGCATTGCAGGTCGCTGAG TGGTTGAAAAAGGTATTTGGAGACCATCCCATCCCACAATATGAGATGAACTCACGGACGACAGAAATTT TATATCACCTTTCAGAACGCAACAGGGTCCGGGACAGGGACATCAGCCTGGTAATAGAGGACTTAAAACA GAAAGCAAGTGAATATGAATCCGAAGCCAAACGTCTTGAAGACTTTCTCATGGAGAGTGTGAATTTTTCA CCTGCCAATCTGTCTAAGTCTGGTTCCCGGTTTCTGAATGCACTGGTAGACAGTGCAATTGCCCTGGAAA TAAAAGATACTTCACTAGCAAGCTTCATCCCCGCAGTGAATGATTTGACGTCTGACCTTTTTCGTACCAA ATCCAAAAGCGAAGAAATGAAGCTTGAACTGGGAAAACTTGAAAAGAACCTGACGGCAACGCTGGTGTTA GAAAAATGTCTACGAGAAGATCTCAAGAAAGCAGAGCTGCAACTGTCTGCAGAAAAGGCCAAGGTTGACA GTCGCCTTCAGAATATGGACTTCCTAAAAGCGAAGGCAGCGGAGTTCCGGTTTGGAATCAAAGCTGCAGA GGAGCAACTTTCAGCCAGAGGCATGGATGCGTCTCTGTCTCATCGGTCACTAGCGGCACTTTCAGAGAAA CTCTCGGAGTTAAAAGAGCAGACTATACCCCTGAAGAAGAAGTTGGAGTCCTATTTAGATTTAATGCCGA GTCCATCTCTTGCTCAACTGAAAATTGAAGAGGCAAAGAGAGAATTAGACGCCATCGAGGCTGAACTTAC CAAGAAAGTAGACATGATGGGCCTGTGAGGACAGCCATGTAGACGTGGTCAGGGGAAGGAGGTGACTGTT CTTAGAGATGATGATGTCTCTATTAGTAAAACAACAGGGTTCGCTTCTGCCTTTAAACTTTGTGGCCTGA ATACAGTTTGCATATGAAAAGGCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5026575 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5026575 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11063 same embryo
	EMAGE:11067 same embryo
	EMAGE:11062 same embryo
	EMAGE:11061 same embryo
	EMAGE:11064 same embryo
	EMAGE:11065 same embryo
	EurExpress:euxassay_001268 same experiment
	MGI:4825326 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source