EMAGE mouse gene expression spatial database

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EMAGE:11067

Col13a1 collagen, type XIII, alpha 1 ( MGI:1277201)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001196. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001196_01	euxassay_001196_02	euxassay_001196_03	euxassay_001196_04

euxassay_001196_05	euxassay_001196_06	euxassay_001196_07	euxassay_001196_08	euxassay_001196_09

euxassay_001196_10	euxassay_001196_11	euxassay_001196_12	euxassay_001196_13	euxassay_001196_14

euxassay_001196_15	euxassay_001196_16	euxassay_001196_17	euxassay_001196_18	euxassay_001196_19

euxassay_001196_20	euxassay_001196_21	euxassay_001196_22	euxassay_001196_23	euxassay_001196_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11067_wholemount_strong.wlz

11067_wholemount_moderate.wlz

11067_wholemount_weak.wlz

11067_wholemount_possible.wlz

11067_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11067_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

weak

regional

weak expression: see section 10 11 12 13 14 15 16 17 18 19 20 21

limb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 09 10 23 24

vibrissa

weak

regional

weak expression: see section 07 08 10 11 12 23 24

external naris

moderate

regional

moderate expression: see section 16

esophagus

weak

regional

weak expression: see section 14 15

meckel's cartilage

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 19 20 22 23 24

lower jaw incisor

moderate

regional

moderate expression: see section 14 15 17 18 19

lower jaw molar

moderate

regional

moderate expression: see section 10 11 21 22 23

upper jaw incisor

moderate

regional

moderate expression: see section 13 14 15 16 19 20

upper jaw molar

moderate

regional

moderate expression: see section 08 09 10 11 21 22 23 24

bladder

weak

regional

weak expression: see section 15 16 17

genital tubercle of male

weak

regional

weak expression: see section 14 15 16 17 18

lung

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

trachea epithelium

weak

regional

weak expression: see section 15 16 17

skeleton

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 15 16 20 21 22 23 24

clavicle

weak

regional

weak expression: see section 09 10 11 12 19 20 21 22

tail

moderate

regional

moderate expression: see section 13 14 15 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4673
Entity Detected:	Col13a1, collagen, type XIII, alpha 1 ( MGI:1277201)
Sequence:	sense strand is shown >T4673 CCCACGCGTCCGGCGCGGCTCACTGCAGCCACGACCCACCTCTGCCCATGGGGCCCTGCATGCGTGCCCC TTCGCCCGGGGACTGACACCCGTTCTCCCCGGAGACACAAAACACCCAGAAGGACCCCGAGCGTCGCACC AGCCCGAGAACTCTGCAGAAGAGAGAAGGGAAAGAGTGGGGAGCGGGGCCAGATCCCCGAACTTGGAACA TTCTTCGAAATAACTTTTTTCTTACTTTGGCGTACTCCGATCGCGTCTGCTGGTGGCTCTTGGCTGCTCC CGACTCCGACTGCTCATTTTCGGTCCTCTTTGGCGAGCAAAGAAAAGGGACGTCCTCTAGCCACGTTAAC CCCCTTTCCCCTTGCCTCTCAGTTTGGATCGCGCCTCTTGGATCTCGTTTTTATTTATTCTATTTATTTA TTTATTGGCTCTCAAGACGCGAGGGCATGGTGGCGGAGCGCACCCGCAAAGCGGCAGCCTCTGGCAGCCG CGGCCCTGGGGAGCTGGGCGCTCCGGGGCCCGGGACAGTGGCGCTGGCTGAGCAGTGCGCTAGGCTGCCG AGCCCCGGGTGCTGCGGACTGCTGGCTCTAGCGCTGTGTTCGCTGGCGCTCAGCCTGCTCGCCCACTTTC GGACCGCGGAGCTGCAGGCCCGGGTGCTGCGCTTGGAAGCAGAGCGCGGGGAGCAGCAGATGGAGAAAGC TATTTTGGGACGAGTCAACCAACTGCTGGACGAGAAATGGAAGTTCTACTCGCGTAGGCGCCGGGAAGCC CCGAAGATGTCTCCAGGATGTAACTGCCCACCAGGACCTCCTGGTCCCACTGGAAGACCTGGACTCCCAG GGGACAAGGGTGCCATCGGGATGCCTGGACGTGTGGGTGCCCCTGGAGATGCGGGGATGTCCATCGTCGG GCCTCGAGGCCCCCCTGGCCAGCCTGGTACCCGAGGTTTCCCTGGATTCCCGGGTCCCATTGGTCTTGAC GGCAGGCCGGGCCACCCCGGACCTAAAGGGGAGATGGGCCTGGTGGGTCCTCGAGGACAACCGGGACCTC AGGGACAAAAGGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACAGGGAGTACCCAGGCGGCATGCTAGC AGCTCTGCGCTCCAACCCGATAATGTCCCTAAAGGGGGAACAAAGTCAGACTGGCATCCAAGGCCCTCCA GGGCCACCGGGCCCCCCAGGACCAAGTGGCCCTCTGGGACACCCAGGACTGCCAGGGCCTATAGGGCCAC CTGGTTTACCGGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTATCATGGCCGCAAGGGAGA GCGGGGCATGCCAGGAATGCCAGGCAAGCACGGAGCCAAGGGGGTTCCTGGGATTGCCGTGGCTGGGATG AAGGGTGAGCCAGGGACGCCAGGAACCAAGGGTGAGAAGGGTGCAGCAGGCTCCCCTGGCCTCCTGGGGC AGAAGGGAGAAAAAGGTGATGCTGGCAATGCCATCGGAGGAGGCAGGGGGGAGCCTGGCCCCCCGGGGCT CCCTGGGCCCCCTGGGCCAAAGGGAGAAGCTGGTGTTGATGGCCAGGCTGGGCCCCCAGGACAGCAAGGA GACAAGGGGCAGCCAGGAGCAGCTGGTGAACAGGGACCCTCTGGCCCCAAGGGTGCCAAGGGTGAACCCG GCAAAGGAGAGATGGTGGATTATAATGGCAGCATCAACGAGGCGCTTCAGGAGATCAGGACGCTGGCCTT GATGGGGCCTCCTGGTCTTCCTGGACAAACAGGCCCACCCGGACCTCCAGGGACTCCAGGCCAAAGGGGG GAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGACAAGGGGCCTCGAGGAAAACCAGGAGATGGGG CCGGCTGGTCCTCAAGGACCCCCAGGAAAGGATGGGCCTCCAGGAATGAAGGGAGAAGTCGGACCCCCAG GAAGTCCAGGGGAGAAAGGGGAGACAGGACAAGCAGGCCCCCAGGGAGAGAAAGGAGAAGCTGGGGAGAA AGGCGACCCGGGAGCAGAGGTTCCTGGGCCGCCAGGGCCAGAGGGTCCTCCAGGACCTCCGGGCCTCCAA GGTTTTCCTGGACCAAAGGGAGAAGCAGGCCTGGAGGGCAGTAAAGGAGAGAAGGGCTCCCAGGGAGAAA AAGGAGACCGGGGGCCTCTGGGATTGCCTGGTACTCCAGGACCAATCGGGGTTCCAGGCCCAGCCGGACC AAAGGGCGAAAGGGGCAGCAAAGGCGACCCAGGGATGACGGGACCAACGGGAGCAGCTGGGCTGCCTGGT TTACATGGACCACCCGGGGACAAAGGAAACCGGGGGGAGAGGGGGAAGAAGGGCTCTAGAGGGCCTAAAG GGGACAAGGGGGACCAAGGAGCACCTGGACTAGACGCCCCCTGCCCGCTGGGAGAAGATGGCTTACCAGT CCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTGGGACTGGCCTGTGTGTGTTTGTATATAGGATATTT ATTTTTATACAGTTTTCACCTTTTGAAAATGCCCAAACTATGATGCATCTTACAGATCATTAAGAAAGAA AGAAGAAAAGCCTGCAGATTTTGTACAGGAAATAACCCCTTTCCCTTTTTGTTTACAAGACATTTTTGTA TAAATCTGTTTCTAATACACTTTCTGTCTGTGCGTCTGCTCCTAATGTTGCATGATATTTGTGCACACTT ACTAAATATCACAGCTTCATAAATTATTGTGTTCCAGTGCCAAAGGGGGCCCACCAGCACAGAGGTGCTG TGAGCTTGTGTGTGAATTCACACACACACACAAAAAAAGTGGAGGTCGGTGGTGTTTGCCAGACTACATG TGTCTGACAATATTTTACATTTTAACTGACGGTCATTATTATAAGAAAAAAATAATAAAACGTGGCAATT CAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5025449 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5025449 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11063 same embryo
	EMAGE:11062 same embryo
	EMAGE:11066 same embryo
	EMAGE:11061 same embryo
	EMAGE:11064 same embryo
	EMAGE:11065 same embryo
	EurExpress:euxassay_001196 same experiment
	MGI:4823976 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source