EMAGE mouse gene expression spatial database

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EMAGE:11141

Bbs9 Bardet-Biedl syndrome 9 (human) ( MGI:2442833)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003475. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003475_01	euxassay_003475_02	euxassay_003475_03	euxassay_003475_04

euxassay_003475_05	euxassay_003475_06	euxassay_003475_07	euxassay_003475_08	euxassay_003475_09

euxassay_003475_10	euxassay_003475_11	euxassay_003475_12	euxassay_003475_13	euxassay_003475_14

euxassay_003475_15	euxassay_003475_16	euxassay_003475_17	euxassay_003475_18	euxassay_003475_19

euxassay_003475_20	euxassay_003475_21	euxassay_003475_22	euxassay_003475_23	euxassay_003475_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11141_wholemount_strong.wlz

11141_wholemount_moderate.wlz

11141_wholemount_weak.wlz

11141_wholemount_possible.wlz

11141_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11141_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adenohypophysis

moderate

regional

moderate expression: see section 11 12 13 14 15 16

3rd ventricle

moderate

regional

moderate expression: see section 12 13 19

choroid invagination

moderate

regional

moderate expression: see section 05 not examined expression: see section 06 08

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 06 07 08 09 10 11 14 15 16 17 18

4th ventricle

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 16 17

facial vii ganglion

strong

regional

strong expression: see section 21 22 moderate expression: see section 04 07

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 20 moderate expression: see section 07 08 09 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 21 22 23 moderate expression: see section 04 05 06 07 08 09 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 10 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 12 18

cervical ganglion

moderate

regional

moderate expression: see section 10 18 19

thoracic ganglion

moderate

regional

moderate expression: see section 13 14 15 17

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 13 16 17 18 19

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 24

not examined

regional

not examined expression: see section 01 02 03 04 22 23 24

neural retina

weak

regional

weak expression: see section 01 02 03 04 23 24

naris

moderate

regional

moderate expression: see section 18

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 13 17 moderate expression: see section 08 09 10 11 12 15 16 18 19

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 13 17 moderate expression: see section 14 15 16

midgut

strong

regional

strong expression: see section 09 13 14 moderate expression: see section 06 07 08 10 11 12 15 16 17 18 19

not examined

regional

not examined expression: see section 10 11 12 13 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6675
Entity Detected:	Bbs9, Bardet-Biedl syndrome 9 (human) ( MGI:2442833)
Sequence:	sense strand is shown >T6675 CCCACGCGTCCGCTCCGTTGTAAAATGCTATGACAACAGGGCGAAGGCGGTTTGGATTCAGGCTGGAGTG GGGAGGTGTCTTCCGCAGGCTGTTGTCTTGCAGCCTCACCCCTCTTACAGTCTCCCTGGTGGTTTCATCC TCTCTGTGAGCATCCGGACTTGGAGTTTTTCTCTCTTGTCACTTGTGGCTTCCGAGGGATCCATGTCGTC TCCCCTGCTTGTCTCTGGGGGATGACAAGAGGGTGCAGGGGATCTTGGGACTCACTTCACCTCCGCGGAG CTTGTGTTGCTGCTTGGCAGGATTCTGTCTGCTTGGGAACGTGGAAAACTTCGAACACAAACAAGACCAA GAGGTTGAAAGGAAATGTCCTTATTTAAAGCCCGTGACTGGTGGTCTACTGTGTTGGGAGAAAAAGAAGA ATTTGACCAGGGCTGCTTATGCTTGGCTGATGTGGACAACAGTGGGAACGGCCACGATAAAATCATTGTG GGCAGCTTCATGGGCTACCTGAGAATCTTTAGTCCCCATTCTGTAAAAGCTGGAGGTGGGCCTCAGGCTG AAGATTTACTTCTAGAAGTGCATCTGCGGGACCCAGTGCTGCAAGTGGAAGTCGGGAAGTTTGTCTCGGG CACCGAGATGCTTCATCTGGCCGTGCTGCACTCCCGGAAGCTCTGTGTCTACTCCGTCTCAGGGACATTG GGAAATGTGGAACATGGAAACCAATATCAGATAAAACTAATGTATGAGCACCATCTTCAGAGGACGGCCT GCAATATGACGTATGGACCCTTTGGTGGTGTAAAAGGTCGGGATTTAATTTGTATCCAGTCTCTGGATGG GATGCTGATGGTGTTTGAGCAGGAAAGCTATGCGTTTGGGAGGTTTCTCCCTGGTTTTCTTCTGCCTGGC CCTCTTGCTTACAGTCCCCGCACGGATTCCTTCATAACTGTGTCTTCTTGCAGACAAGTGGAAAGTTACA AGTACCAGGTACTTGCCTTTGCCACAGATGCAGACAAGAAGCAGGAGATGGAACAGCAAAAACTTGGTTC TGGAAAGCGGCTAGTTGTGGACTGGACTCTGAATATTGGCGAGCAAGCCCTTGATATCTGTATCTTCCCT TTGAACCAATCTGCATCTTCTGTTTTTGTTCTTGGTGAGAGAAACTTCTTTTGCCTAAAGGATAATGGGC AGATTCGATTCATGAAGAAGCTCGGTTATAACCCCAGTTGTTTTCTACCGTACTGCTCAGTTTCTGAAGG TACAATAAATACTTTGATTGGAAACCATAACCACATGCTGCATATTTACCAGGACGTGACACTGAAGTGG GCCACACAACTCCCCCACGTCCCCGTGGCCGTCAGAGTAGGCTGTTTTCATGATTTGAAGGGAGTGATAG TGACTCTCAGTGATGACGGTCACCTGCAGTGCTCGTATCTGGGGACAGACCCTTCTTTGTTCCAGGCTCC AAAAGTTGAATCAAGAGAGCTCAACTATGATGAACTTGATGTGGAATTGAAAGAACTTCAGAAAATCATC AAAGATGTAAAGTTGCAAGGTGTTTGGCCCTTGACTGAACAGGAAGATGACTTGAAAGTCTCTGCTTCAG TGTCTTCTACCCTGGATTCTGTGTCTCAAGCCACCAATGTGGAACCTGGAGCTGACTCTGTCCCGTCCAT CACAGTGAAGATTACCCTGCAGAACCGAGTGGTGTTGCAGAAGGTCAAGCTGTCTATCTATGTGCAGCCT CCACTACAGTTGACTTGTGATCAGTTCACCTTTGACTTCACGGTTCCAGATATGACGAGTTCAGTAGCGT TTTCTGTGTACCTGAAGAGAAACTACACACCATCCGAGTTAGAAGGAAATGCTGTTGTTTCTTACTCCAG ACCGACAGGTATTCCTCGGGTTGTCCAATGTAAATTTAGACTTCCTCTGAAACTGATTTGCCTACCTGGT CAGCCTTCAAAAACTGCAAGCCACAAACTAACTATAGACACCAACAAATCTCCTGTCAGTCTGCTCGGTC TCTTCCCAGACTTTGCCAACCCATCAGATGACGACCAAGTGAATGTAATGGGCTTCCGTCTCCTAGGAGG CGCTCGAGTGACTCTTCTTGCTTCCAGAACATCCCAACGATACCGCATTCAGAGCGAACAGTTTGAAGAT CTCTGGCTCATAACCAATGAACTCATCCTCCGCCTTCAGGAACATTTTGAAAAACAGGGAACCAAAGACT TTTCGTGTTCTTTTTCTGGGTGTGTGCCCCTTCAAGAATATTTTGAGTTGATTGATCATCATTTTGAGCT GCGGATAAATGGTAAAAAATTGGAAGAACTTTTATCTGAAAGAGCTGTACAATTTCGGGCCATTCAGCGT CGGCTTCTGACAAGATTCAGAGACAAGACCCCTGCCCCACTGCAGCACCTGGACACCCTGCTGGACGGGA CATACAAGCAGGTGATTGCTCTAGCAGATGCGATCGAGGAGAACCAGGACAGGCTGTTGCAGTCATTCTC AGGCCTGAAGAGTGCCACCCATTTGCTGATCCTGCTAATCAGGCTGTGGCAGAGGCTGAGCGCTGACCAG ACTGCTATTCTGGAAGCAGCATTTCTGCCACTACAGGAAGACACGCAGGAACTGGGCTGGGAAGAAACCG TGGATGCTGCCATTGCCTACCTTTTGAAAACCTGCCTGTCGAAGAGTTCCAAGGAGCAGGCTTTGAACCT CAGCAGCCAACTGAACATACCCAAAGATACCAGCCGGCTGAAGAAGCACATCACCCTGCTGTGTGATAGG TTAGCCAAAGGTGGGCGTCTCTGCGTGAGCACAGATGCAGCGGCCCCGCAGGCCATGGTTGTGCCAGGTG GCTGTACTCCAATCCCAGAGTCAGACCTAGAGGAAAGGTCACTAGATGACTCCACAGAGCTGTTTACCAA CCACAAGCACCTCATGACCGAGCCCCCCATGCCTGAAGTCTCAGCCCGCCAAGGAGTTTTGGAATAGTCT GGAGTAATGCTAATGAGTTGAGGGGAGAGGCCATTACAGAGGCTACCATGTCTGGACACTGTGTTCCCAT CCCGGTGCTGTGCCAAGCTCATCTCTGGAGGTGACTGCGTGAAGATGGGGTCAGGGCTGTTGGTTGCTAG GAGGGCGTGGGATGTCACAGACAGGTCAGCTGAAAGACAAGATTTGTCTTTTCTTCCCAAGAAAATGGTT GCTGCCTTTAGTCACATGCGCTTATAGTCAGGATTTAGCAAGGTGACGAAGTTTGGGGACAGACTTTATA ATACAGGGATCCAGATGTAAAATAATTTCATAGCCCATTTTTTTCCACAGGATTCTTATGTAGTAACTGT TTCAAGTTTAATAAAGCATCGTATTGTCAAACAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3601505 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601505 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11142 same embryo
	EMAGE:11144 same embryo
	EMAGE:11143 same embryo
	EMAGE:11145 same embryo
	EMAGE:11140 same embryo
	EurExpress:euxassay_003475 same experiment
	MGI:4823399 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source